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Kit for highly sensitive detection of hydrazine and its application

A kit and a technology for detecting samples, which are applied in the direction of measuring devices, instruments, and analytical materials, can solve the problems of complex synthesis, poor selectivity, and low sensitivity, and achieve the effect of simple synthesis and good stability

Active Publication Date: 2017-11-07
杭州百裕生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, fluorescent probes have been rapidly developed due to their advantages of high sensitivity and convenient operation, but there are still some deficiencies in the reported hydrazine fluorescent probes, such as poor selectivity, low sensitivity, slow response, complex synthesis, and excitation and emission. relatively short wavelength

Method used

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  • Kit for highly sensitive detection of hydrazine and its application
  • Kit for highly sensitive detection of hydrazine and its application
  • Kit for highly sensitive detection of hydrazine and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022]

[0023] (Scheme 1) 4-hydroxystyrene tricyanofuran (303mg, 1.0mmol), 4-bromobutanoic acid (166mg, 1.0mmol), 4-dimethylaminopyridine (122mg, 1.0mmol) and dicyclohexyl Carbodiimide (412mg, 2.0mmol) was dissolved in 20mL of dry dichloromethane, reacted at 50°C for 4h, evaporated under reduced pressure to obtain crude product, and then separated by column chromatography using dichloromethane to obtain 140mg of light yellow pure product , and the yield was 31%.

[0024] (Scheme 2) 4-hydroxystyrenetricyanofuran (303mg, 1.0mmol), 4-bromobutanoic acid (166mg, 1.0mmol), 4-dimethylaminopyridine (122mg, 1.0mmol) and dicyclohexyl Carbodiimide (412mg, 2.0mmol) was dissolved in 20mL of dry dichloromethane, reacted at 50°C for 10h, evaporated under reduced pressure to obtain crude product, and then separated by column chromatography using dichloromethane to obtain 293mg of light yellow pure product , and the yield was 65%.

[0025] (Scheme 3) 4-hydroxystyrenetricyanofuran (303mg,...

Embodiment 2

[0029] The inventor of the present invention has carried out following test: (a) the impact of different concentrations of hydrazine (0~500 μ M) on the absorption spectrum of probe (20 μ M), insert figure is the ratio of the absorbance at 595nm place and the absorbance at 410nm place and the concentration of hydrazine added linear relationship between. (b) Effects of different concentrations of hydrazine (0-500 μM) on the fluorescence spectrum of the probe (20 μM); the insert graph is the linear relationship between the fluorescence intensity at 610 nm and the concentration of added hydrazine. The above determinations were made in a system containing ethanol and water (5:5, V / V) containing 5mM phosphate buffered saline (PBS) with a pH value of 7.4, and all spectral tests were performed at 25°C after adding hydrazine for 20 minutes. of. See results figure 1 .

[0030] From figure 1 a It can be seen that with the increase of the concentration of hydrazine in the probe soluti...

Embodiment 3

[0032] Depend on figure 2 It can be clearly seen that the color of the probe solution (40 μM) gradually changes from yellow to blue in the presence of different concentrations of hydrazine (from left to right: 0, 0.2, 0.4, 0.6, 0.8, 1.0 mM). Therefore, the probe of the present invention can complete the qualitative and quantitative analysis and detection of hydrazine in the sample only by "naked eye" observation.

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Abstract

The invention relates to high-sensitivity hydrazine detection kits and application thereof, in particular to kits with 4-bromobutyrate tricyano furan styrene compounds serving as long-wavelength hydrazine colorimetric fluorescent probes. The probes are not only high in selectivity in hydrazine recognition but also flexible in hydrazine qualitative and qualitative analysis carried out at long-wavelength positions according to a fluorescence method. The probes are capable of quickly responding to hydrazine to realize real-time hydrazine detection and capable of realizing hydrazine qualitative and qualitative analysis only by 'naked eye' observation and are available for long-term storage and use due to property stability. In addition, the probes have excellent anti-interference performances. Synthesis conditions of the probes are mild relatively, and the probes can be synthesized simply only by one-step reaction.

Description

technical field [0001] The invention relates to a 4-bromobutyrate tricyanofuran styrene compound as a hydrazine highly selective long-wavelength colorimetric fluorescent probe and its application. Background technique [0002] Hydrazine (NH 2 NH 2 ) is widely used in industrial fields such as organic synthesis because of its strong reducibility. In addition, hydrazine has also been applied in the field of medicine as an antibacterial drug. However, studies have shown that hydrazine is highly toxic and can increase the incidence of lung cancer, rhinitis cancer, and liver cancer. Now hydrazine has been listed as a potential human carcinogen by the US Environmental Protection Agency and the World Health Organization. As we all know, hydrazine has strong volatility and is easy to cause environmental pollution including air and water. Therefore, it is necessary to develop a method for the detection of hydrazine with high selectivity and sensitivity. [0003] At present, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/68G01N21/64
CPCC07D307/68G01N21/6428
Inventor 叶龙武张艳
Owner 杭州百裕生物科技有限公司