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Colorimetric fluorescent probe for ultrasensitive analysis of mercury based on resorufin

A technology of hydroxyl and sulfonic acid groups, which is applied to the analysis of materials, fluorescence/phosphorescence, and material excitation analysis. It can solve the problems of complex synthesis, insufficient selectivity, and insufficient response speed, etc., and achieve simple synthesis, good stability, To achieve the effect of selective recognition

Inactive Publication Date: 2018-10-12
李新元
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems with the currently reported colorimetric and fluorescent probes, including insufficient selectivity, insufficient response speed, and complex synthesis.

Method used

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  • Colorimetric fluorescent probe for ultrasensitive analysis of mercury based on resorufin
  • Colorimetric fluorescent probe for ultrasensitive analysis of mercury based on resorufin
  • Colorimetric fluorescent probe for ultrasensitive analysis of mercury based on resorufin

Examples

Experimental program
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Effect test

Embodiment 1

[0032]

[0033] (Scheme 1) Dissolve 200mg (0.71mmol) of resorufin compounds in 15mL of dichloromethane, then add 245.7mg (1.42mmol) of phenyl thiochloroformate and stir for 2h at room temperature, then use a rotary evaporator to carry out rotary evaporation column to obtain crude product. If a purer product is to be obtained, it can be passed through a column and then recrystallized in a mixed system of dichloromethane and petroleum ether (eg v / v, 1:5) to obtain a pure product. Obtained 210mg of orange pure product, the yield was 71%.

[0034] (Scheme 2) Dissolve 200mg (0.71mmol) of resorufin compounds in 15mL of dichloromethane, then add 184mg (1.07mmol) of phenyl thiochloroformate and stir at room temperature for 2h, then use a rotary evaporator to perform rotary evaporation to obtain crude product. If a purer product is to be obtained, it can be passed through a column and then recrystallized in a mixed system of dichloromethane and petroleum ether (eg v / v, 1:5) to obt...

Embodiment 2

[0039] figure 1 is the probe (5μM) added with Hg 2+Fluorescence spectra before and after (20μM), through the illustration we can see that the fluorescence changes before and after adding mercury ions are very obvious. It shows that the probe has a good response to mercury ions, and the probe can realize sensitive recognition of mercury ions.

Embodiment 3

[0041] Figure 2a are different concentrations of Hg 2+ (0-100nM) on the probe (5μM) fluorescence spectrum; Figure 2b are different concentrations of Hg 2 + (0-10μM) versus probe (10μM) UV absorption spectrum.

[0042] It can be seen that with the Hg in the probe solution 2+ As the concentration increases, the fluorescence intensity gradually increases, and at (0-100nM)Hg 2+ Concentration range, Hg 2+ The concentration has a good linear relationship with the fluorescence intensity. The standard limit of mercury ions in my country's drinking water standards is 0.001 mg / L. Therefore, the probe of the present invention can more accurately determine the content of mercury ions in the sample to be tested. In addition, it can be seen from the ultraviolet spectrum that the absorbance increases gradually with the increase of the concentration of mercury ions, indicating that the probe can be used as a colorimetric probe to detect mercury ions.

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Abstract

The present invention relates to a colorimetric fluorescent probe for ultrasensitive analysis of mercury based on resorufin. Specifically, the probe is a class of resorufin-like compounds and can be used as a mercury ion colorimetric fluorescent probe for detection of mercury ions. The probe can achieve at least one of the following technical effects: high-selective identification of the mercury ions, ultrasensitive analysis of the mercury ions, stable property, long-term preservation and use and relatively strong anti-interference ability.

Description

technical field [0001] The invention relates to a resorufin compound used as a fluorescent probe for mercury ions, which can rapidly and sensitively identify mercury ions with high selectivity, or it can measure the concentration of mercury ions in a sample. Background technique [0002] Mercury is a metal element with severe physiological toxicity. Due to its persistence, easy migration and high bioaccumulation, it has become one of the most concerned environmental pollutants. Inorganic mercury ions in the environment can be converted by organisms into highly toxic methylmercury under certain conditions. Inorganic mercury mainly affects the kidneys, while methylmercury mainly damages the nervous system after entering the human body, especially the central nervous system. Both can be highly enriched in biological tissues through the food chain, thus causing great harm to humans and nature. Mercury poisoning will have an extremely bad impact on the whole society. Mercury is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D265/38C09K11/06G01N21/64
CPCC07D265/38C09K11/06C09K2211/1033G01N21/6428
Inventor 李新元朱宝存
Owner 李新元
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