Detection device for simultaneously and quantitatively detecting SAA/PCT/CRP

A quantitative detection and detection device technology, applied in the field of medical detection, can solve problems such as increased CRP content, and achieve the effects of low cost, high detection sensitivity, and simple and stable detection methods

Inactive Publication Date: 2016-03-23
天津中新科炬生物制药股份有限公司
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during inflammatory disease activity in autoimmune dis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection device for simultaneously and quantitatively detecting SAA/PCT/CRP
  • Detection device for simultaneously and quantitatively detecting SAA/PCT/CRP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1 main material

[0049] 1.1 Biological materials SAA, PCT and CRP paired antibodies, purchased from Finland HYTEST company; goat anti-mouse IgG: homemade; chloroauric acid: Sigma company product; NC membrane: Sartorius company product; bovine serum albumin (BSA), polyethylene Alcohol PEG20000: Sigma product. Other commonly used reagents are analytical reagents.

[0050] 1.2 Clinical samples were obtained by the company in related hospitals, a total of 120 samples, of which 60 were serum samples of patients diagnosed with inflammatory infection, the SAA detection value was 5-200mg / L, the PCT detection value was 0.5-20mg / L and 1-200mg / L. In 60 normal human serum samples, the SAA, PCT and CRP values ​​were not detected.

[0051] 1.3 Triple test card: designed by our company, and produced and provided by related companies as required.

[0052] 1.4 Immune Chromatography Results Interpretation Recorder: Model: NS3001, a product of Tianjin Zhongxin Keju Biopharmaceutical Co., Ltd...

Embodiment 2

[0067] A test strip for simultaneous rapid and quantitative detection of SAA / PCT / CRP is prepared by the following method. The specific steps are:

[0068] (1) SAA, PCT and CRP antibody colloidal gold labeling. Prepare a colloidal gold solution with a diameter of 40±5nm by the chloroauric acid-trisodium citrate method. Take three colloidal gold solutions and adjust the solution to SAA with 0.2MK2CO3. Adjust the pH of PCT to 7.5, the pH of PCT to 6.5 and the pH of CRP to 8.0, and then slowly stir the colloidal gold. SAA: add 10ug per ml of solution to add SAA-labeled antibody to the solution; PCT: per ml of solution Add 15ug to add PCT-labeled antibody to the solution; CRP: add 5ug per ml of solution to add CRP-labeled antibody to the solution, continue to stir for 30 minutes, and then add to the final concentration of 0.5% PEG2000 and 0.5% bovine serum albumin (BSA) for blocking, after labeling, centrifuge at 13000r / min, discard the supernatant, and reconstitute the precipitate in...

Embodiment 3

[0072] (1) SAA, PCT and CRP antibody colloidal gold labeling. Prepare a colloidal gold solution with a diameter of 40±5nm by the chloroauric acid-trisodium citrate method. Take three colloidal gold solutions and adjust the solution to SAA with 0.2MK2CO3. Adjust the pH of PCT to 7.0, the pH of PCT to 7.0 and the pH of CRP to 7.0, and then slowly stir the colloidal gold. SAA: Add 8ug per ml of solution to add SAA-labeled antibody to the solution; PCT: per ml of solution Add 18ug to add PCT-labeled antibody to the solution; CRP: add 8ug per ml of solution to add CRP-labeled antibody to the solution, continue to stir for 30 minutes, and then add to the final concentration of 0.5% PEG2000 and 0.5% bovine serum albumin (BSA) for blocking, after labeling, centrifuge at 13000r / min, discard the supernatant, and reconstitute the precipitate into colloidal gold working solutions of different proportions (0.1MTris.HCl buffer, pH7.8, containing 70% of the original volume) 0.2% bovine serum ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Widthaaaaaaaaaa
Lengthaaaaaaaaaa
Login to view more

Abstract

The invention provides a detection device for simultaneously and quantitatively detecting SAA/PCT/CRP. The detection device for simultaneously, rapidly and quantitatively detecting SAA/PCT/CRP is composed of human serum amyloid A (SAA) test paper, human procalcitonin (PCT) test paper, C-reactive protein (CRP) test paper, a triplicate card case and an immunochromatography interpretation result recorder carrying a corresponding judgment method and a standard curve, wherein the human serum amyloid A (SAA) test paper, the human procalcitonin (PCT) test paper and the C-reactive protein (CRP) test paper are independent. The detection device is suitable for on-site detection, a detection method is easy, convenient and stable, the situation that a large number of blood samples are extracted is not needed, medical cost is reduced, sensitivity is high, and SAA/PCT/CRP can be simultaneously, rapidly and quantitatively detected.

Description

Technical field [0001] The invention relates to the field of medical detection, in particular to a detection device for simultaneous quantitative detection of SAA / PCT / CRP. Background technique [0002] Serum amyloid A (SAA) is a highly heterogeneous acute phase reactive protein, mainly produced by hepatocytes, and its relative molecular weight is about 12,000. The concentration of SAA, an acute reactant in the inflammatory response, will be 1000 times higher than that in normal conditions, and the increase is higher than that of CRP. At this time, it can replace the binding with HDL through its N-terminal and high-density lipoprotein (HDL) binding. Apolipoprotein AI (ApoA-I) forms the acute phase SAA / HDL complex, and its half-life is only about 50 minutes. Studies have shown that in the case of severe inflammation, the level of SAA expression is closely related to the number of necrotic cells and tissues. The expression of SAA peaked on the 3rd day of inflammation and returned ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/569
CPCG01N33/569
Inventor 李洲周洪锐陈逸桐张梓琪李昀地杨延瑞
Owner 天津中新科炬生物制药股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap