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Dengue Ⅰ, Ⅱ, Ⅲ, ⅳ type rt-pcr one-step mix detection kit and its detection method

A RT-PCR, detection kit technology, applied in biochemical equipment and methods, microbial determination/inspection, resistance to vector-borne diseases, etc. Realize problems such as early screening and rapid diagnosis, and achieve the effect of simple operation

Active Publication Date: 2019-01-15
深圳生科原生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to provide a one-step RT-PCR MIX detection kit for dengue fever types I, II, III, and IV, which aims to solve the problem that the existing dengue fever detection methods are time-consuming, cannot realize early screening and rapid diagnosis, and cannot be detected in one tube. Simultaneous detection of dengue fever types Ⅰ, Ⅱ, Ⅲ, and Ⅳ

Method used

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  • Dengue Ⅰ, Ⅱ, Ⅲ, ⅳ type rt-pcr one-step mix detection kit and its detection method
  • Dengue Ⅰ, Ⅱ, Ⅲ, ⅳ type rt-pcr one-step mix detection kit and its detection method
  • Dengue Ⅰ, Ⅱ, Ⅲ, ⅳ type rt-pcr one-step mix detection kit and its detection method

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Embodiment 1

[0093] A one-step RT-PCR MIX detection kit for types I, II, III, and IV of dengue fever, comprising a PCR reaction solution, a negative control and a positive control, wherein the specifications of the RT-PCR reaction solution are components as shown in Table 1 below; The positive control components include the genomes of dengue fever types I, II, III, and IV, which are diluted with Tris-EDTA buffer (0.01M pH8.0) and stored frozen; the negative control components do not contain the wild strain of classical swine fever virus. and Tris-EDTA buffer (0.01M pH 8.0) of the genomic RNA of the vaccine strain.

[0094] Table 1

[0095]

[0096] The dengue fever type I, II, III, and IV RT-PCR one-step MIX detection method uses the above-mentioned dengue fever type I, II, III, and IV RT-PCR one-step MIX detection kit for detection, including the following steps:

[0097] S11. Sample RNA extraction: take 20 samples to be tested;

[0098] S12. RT-PCR one-step detection;

[0099] S13....

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Abstract

The invention belongs to the technical field of virus detection kit, and provides a dengue I, II, III, IV-type RT-PCR one-step MIX detection kit, and a detection method thereof. The dengue I, II, III, IV-type RT-PCR one-step MIX detection kit comprises dengue I, II, III, IV-type primers and gene probes; and in the detection method, the dengue I, II, III, IV-type RT-PCR one-step MIX detection kit is adopted for detection. Compared with the prior art, the dengue I, II, III, IV-type RT-PCR one-step MIX detection kit and the detection method are capable of realizing detection of I, II, III, IV-type dengue in one step at the same time, sensitivity and specificity are high, detection period is short, and accuracy is high.

Description

technical field [0001] The invention belongs to the technical field of virus detection kits, and in particular relates to a one-step RT-PCR MIX detection kit for dengue fever types I, II, III and IV and a detection method thereof. Background technique [0002] Real-time fluorescent quantitative PCR is widely used in basic scientific research, clinical diagnosis, disease research and drug development and other fields. The basic principle is: a fluorescent chemical substance is introduced into the PCR reaction system, and as the PCR reaction progresses, the PCR reaction product accumulates continuously, and the fluorescence signal intensity also increases proportionally. After each cycle, a fluorescence signal intensity is collected, so that the change of the product amount can be monitored through the change of the fluorescence intensity, and a fluorescence amplification curve is obtained, such as figure 1 As shown, in the exponential amplification stage of the curve, there ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686
CPCC12Q1/686C12Q1/70C12Q2563/107C12Q2537/143C12Q2531/113Y02A50/30
Inventor 吴成贡田仁鹏王茵茵邓春兴
Owner 深圳生科原生物有限公司