Dosage-controllable microinjection device and operation method thereof

A technology of microinjection and operation method, which is applied in the field of controllable dose microinjection device, can solve the problem that the injection dose is difficult to control accurately, and achieve the effects of promoting application, reducing equipment cost, and improving survival rate

Inactive Publication Date: 2016-04-13
HOHAI UNIV CHANGZHOU
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is: to overcome the deficiencies of the prior art, to provide a controllable d

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  • Dosage-controllable microinjection device and operation method thereof
  • Dosage-controllable microinjection device and operation method thereof
  • Dosage-controllable microinjection device and operation method thereof

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[0026] In order to make the content of the present invention easier to be understood clearly, the following further describes the present invention in detail based on specific embodiments in conjunction with the accompanying drawings.

[0027] Such as Figure 1~8 As shown, a controllable dose microinjection device is provided with a microinjection microfluidic channel 202 inside, and a microinjection needle 9 is fixed at one end of the microinjection microfluidic channel 202 , The needle of the microinjection needle 9 faces the other end of the microinjection microchannel 202, the end of the microinjection microchannel 202 where the microinjection needle 9 is fixed is equipped with a first electrode 801, and the microinjection microchannel A second electrode 501 is installed at the other end of the channel 202 to be suitable for controlling the movement of the cells and for controlling the dose of the foreign substance to be injected into the cells in the microinjection needle.

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Abstract

The invention discloses a dosage-controllable microinjection device and an operation method thereof. A microinjection micro-channel is formed inside the microinjection device, a microinjection needle is fixed to one end of the microinjection micro-channel, a first electrode is installed on the microinjection micro-channel, and a second electrode is installed at the other end of the microinjection micro-channel. The invention further provides an operation method of the dosage-controllable microinjection device. The operation method includes the following steps that an alternating current is applied to the first electrode, the frequency of the alternating current is adjusted, and cells are punctured by the microinjection needle; the alternating current on the first electrode is converted into a direct current, the second electrode is grounded, and a to-be-injected exogenous substance solution is injected into the cells through the microinjection needle under the effect of electroosmotic flow. According to the dosage-controllable microinjection device and the operation method, the microinjection needle is fixed, the cells are subjected to accurate fixed-dosage injection with the electroosmosis technology, and the problem that in a traditional method, as the exogenous substance solution in the microinjection needle is driven through pressure to move, the injection dosage cannot be accurately controlled is solved.

Description

technical field [0001] The invention relates to a controllable dose microinjection device and an operation method thereof, belonging to the technical field of cell biology research. Background technique [0002] Microinjection technology is a technology that uses glass microinjection needles to inject foreign substances into cells after adjusting and fixing the injected cells in the field of view of the microscope. It is one of the important technical means of modern cell engineering, especially transgenic, cloning In addition, it has important applications in fields such as drug screening and testing. Compared with other techniques, microinjection has the advantages of stable injection efficiency, unlimited injection gene size, no need for vectors, and special requirements for objects to be manipulated, etc. be widely used. The existing microinjection device fixes the cells through the needle holder connected to the electromechanical device with high displacement resoluti...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/26
CPCC12M33/04C12M35/02
Inventor 吕品朱晓璐纪爱敏王祥冰刘源
Owner HOHAI UNIV CHANGZHOU
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