Dosage-controllable microinjection device and operation method thereof

Abstract

The invention discloses a dosage-controllable microinjection device and an operation method thereof. A microinjection micro-channel is formed inside the microinjection device, a microinjection needle is fixed to one end of the microinjection micro-channel, a first electrode is installed on the microinjection micro-channel, and a second electrode is installed at the other end of the microinjection micro-channel. The invention further provides an operation method of the dosage-controllable microinjection device. The operation method includes the following steps that an alternating current is applied to the first electrode, the frequency of the alternating current is adjusted, and cells are punctured by the microinjection needle; the alternating current on the first electrode is converted into a direct current, the second electrode is grounded, and a to-be-injected exogenous substance solution is injected into the cells through the microinjection needle under the effect of electroosmotic flow. According to the dosage-controllable microinjection device and the operation method, the microinjection needle is fixed, the cells are subjected to accurate fixed-dosage injection with the electroosmosis technology, and the problem that in a traditional method, as the exogenous substance solution in the microinjection needle is driven through pressure to move, the injection dosage cannot be accurately controlled is solved.

Description

technical field [0001] The invention relates to a controllable dose microinjection device and an operation method thereof, belonging to the technical field of cell biology research. Background technique [0002] Microinjection technology is a technology that uses glass microinjection needles to inject foreign substances into cells after adjusting and fixing the injected cells in the field of view of the microscope. It is one of the important technical means of modern cell engineering, especially transgenic, cloning In addition, it has important applications in fields such as drug screening and testing. Compared with other techniques, microinjection has the advantages of stable injection efficiency, unlimited injection gene size, no need for vectors, and special requirements for objects to be manipulated, etc. be widely used. The existing microinjection device fixes the cells through the needle holder connected to the electromechanical device with high displacement resoluti...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is: to overcome the deficiencies of the prior art, to provide a controllable dose microinjection device and its operation method, so as to solve the technical problem that the injection dose is difficult to control precisely

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