Eurotium cristatus and application thereof
A coronoid saccharomyces, a technology for use, applied in the biological field, can solve the problems of less research on aroma-producing substances, no articles or patents, etc., and achieve easy cultivation and preservation and industrial production, without destroying ecological balance, and low production costs Effect
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[0029] 1. Isolation method of FBKL3.0002 (EurotiumcristatusFBKL3.0002) strain:
[0030] Weigh 10g Daqu sample from a distilled koji from a winery in Guizhou and put it in a Erlenmeyer flask containing 90 mL of sterile water and glass beads, and shake for about 30 minutes. Take 1mL sample suspension and carry out 10-fold gradient dilution, respectively by 10 -4 , 10 -5 Spread 1 mL of each diluted sample suspension on the separation medium, culture it at 30°C for 5 days, pick a single colony for further screening, and store it on the slant medium.
[0031] 2. Strain identification method:
[0032] The isolated and purified strains were inoculated on CA medium, cultured in an incubator at 25°C for 5-20 days, and the morphological characteristics of the colonies were observed. After picking a small amount of bacteria for pretreatment, the slides were observed under S-3400N scanning electron microscope The morphological characteristics of the hyphae and spores were morphologically identi...
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