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Eurotium cristatus and application thereof

A coronoid saccharomyces, a technology for use, applied in the biological field, can solve the problems of less research on aroma-producing substances, no articles or patents, etc., and achieve easy cultivation and preservation and industrial production, without destroying ecological balance, and low production costs Effect

Inactive Publication Date: 2016-04-13
GUIZHOU UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, Monascus purple is mainly used to produce ester aroma. There are few studies on aroma-producing substances in Maotai-flavored wine, and there are no related articles or patents.

Method used

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  • Eurotium cristatus and application thereof
  • Eurotium cristatus and application thereof

Examples

Experimental program
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Embodiment Construction

[0029] 1. Isolation method of Eurotium cristatus FBKL3.0002 strain:

[0030] Weighed 10g of Daqu sample from a brewery in Guizhou, put it into a Erlenmeyer flask filled with 90mL of sterile water and glass beads, and oscillated for about 30min. Take 1mL sample suspension for 10-fold serial dilution, respectively by 10 -4 、10 -5 Take 1 mL of the diluted sample suspension and spread it on the separation medium, culture it statically at 30°C for 5 days, pick a single colony for further screening, and store it on the slant medium.

[0031] 2. Strain identification method:

[0032] Inoculate the isolated and purified strains on CA medium, culture them in a 25°C incubator for 5-20 days, observe the morphological characteristics of the colonies, pick a small amount of bacteria for pretreatment, and observe under the S-3400N scanning electron microscope The morphological characteristics of mycelium and spores were identified as Eurotium cristatus strain through "Chinese Fungi" and ...

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Abstract

The invention discloses eurotium cristatus and application thereof. The eurotium cristatus is morphologically characterized in that strains of the eurotium cristatus grow slowly on a CA culture medium; base hyphae are thin, and after hyphae grow on the culture medium by 1-2mm, margins of new colonies and old colonies are in a color of olive light yellow while center portions are in a dark color similar to olive drab to clove brown; a large quantity of yellow hypae emerge and become brown after being old; a large quality of cleistothecia are generated and in a color of yellow; diameters of the colonies are 50-60mm after 20 days, colors of the colonies are completely in a color of olive drab to clove brown, and an extremely small quantity of conidium structures exist; back surfaces of the colonies are in a color of black brown. The eurotium cristatus is a flower-fruit aroma generating substance for promoting flavor of wines.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a coronoid fungus and the use of the coronoid fungus in producing floral and fruity aroma substances. Background technique [0002] Maotai-flavored liquor is the main flavor-type liquor in China, and its unique brewing process has formed its unique sauce-flavored flavor. At present, there is no unified conclusion on the main aroma components of Maotai-flavored liquor. This situation is mainly due to the fact that many organic substances remain in the body of Maotai-flavored liquor during the formation process, so it is difficult to determine the flavor of Maotai-flavored liquor. main fragrance ingredients. The complex composition of the wine body also shows the diversity of aroma substances, and the joint action of various aroma substances forms the unique flavor of Maotai-flavored liquor. [0003] Mold also has a certain aroma-producing function and contributes to th...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12G3/02C12R1/645
Inventor 王晓丹邱树毅陈琳琳徐佳
Owner GUIZHOU UNIV
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