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Process for extracting glutamine from fermentation broth

A technology of glutamine and a process method is applied in the field of preparing amino acids for medicinal and health care purposes, and can solve the problems of low product cost and low energy consumption, and achieve the effects of reducing dosage, reducing production cost and avoiding conversion problems.

Inactive Publication Date: 2016-04-20
刘振宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the existing ion exchange method, the purpose of the present invention is to provide a process for extracting glutamine from fermentation broth with high yield and purity, low product cost, low energy consumption and environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The main components of glutamine fermentation broth are: glutamine 35g / l, glutamic acid 8g / l, ammonium sulfate 50g / l, ammonium chloride 10g / l, a small amount of other inorganic salts, pH 6.7, fermentation broth volume 5L .

[0021] 5g of cationic polyacrylamide was used as a flocculant, added into the fermentation broth under slow stirring, then stirred rapidly for 10 minutes, left to stand for 30 minutes, and filtered. Wash the solid phase with 500 ml deionized water. The supernatant and the washing water are combined and then ultrafiltered through a tubular membrane module. The molecular weight cut off of the ultrafiltration membrane is 6000. Adjust the fermentation broth with 2M sulfuric acid P H is 6, and electrodialysis is performed. The electrodialyzer consists of two positive membranes and one negative membrane. In this process, control the pH of the fermented liquid to be stable at about 6 with 2M sulfuric acid and 2M sodium hydroxide, and control the tempera...

Embodiment 2

[0023] The main components of glutamine fermentation broth are: glutamine 32g / l, glutamic acid 7g / l, ammonium sulfate 50g / l, ammonium chloride 6g / l, a small amount of other inorganic salts, pH 6.6, fermentation broth volume 5L .

[0024] 6g of chitosan was used as flocculant, added into the fermentation broth under slow stirring, then stirred rapidly for 15 minutes, left to stand for 30 minutes, and filtered. Wash the solid phase with 500 ml deionized water. The supernatant and the washing water are combined and ultrafiltered through a flat-panel membrane ultrafiltration device, and the molecular weight cut-off of the ultrafiltration membrane is 10,000. Use 2M hydrochloric acid to adjust the pH of the fermentation broth to 5, and perform electrodialysis. The electrodialyzer consists of two positive membranes and one negative membrane. In this process, use 2M hydrochloric acid and 20% ammoniacal liquor to control the pH of the fermented liquid to be stable at about 5, and con...

Embodiment 3

[0026] The main components of glutamine fermentation broth are: glutamine 38g / l, glutamic acid 10g / l, ammonium sulfate 40g / l, ammonium chloride 20g / l, a small amount of other inorganic salts, P H6.7, the volume of fermentation broth is 5L.

[0027] 7g of non-ionic polyacrylamide was used as a flocculant, added to the fermentation broth under slow stirring, then stirred rapidly for 15 minutes, left to stand for 30 minutes, and filtered. Wash the solid phase with 500 ml deionized water. The supernatant and the washing water are combined and ultrafiltered through a hollow fiber membrane device, and the molecular weight cut-off of the ultrafiltration membrane is 30,000. Use 2\1 nitric acid to adjust the fermented liquid to 6.5, and perform electrodialysis. The electrodialyzer consists of two positive membranes and one negative membrane. In this process, control the pH of the fermented liquid to be stable at about 6.5 with 2M sulfuric acid and 2M sodium hydroxide, and control the...

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Abstract

The invention relates to a process for extracting glutamine from fermentation broth, and belongs to the technical field of preparation of amino acid for medicinal and healthcare applications. The method comprises the following steps: preprocessing glutamine fermentation broth to remove bacterium bodies and part of proteins, pigment, and polysaccharide; after the pretreatment, controlling the pH value of the fermentation broth, removing inorganic salts from the fermentation broth by an electrodialysis facility, readjusting the pH value of the fermentation broth, introducing the fermentation broth into a prepared OH type anion exchange column, wherein the impurities are exchanged with resin and adsorbed by the column, and the glutamine is separated from the fermentation broth after the fermentation broth flows through the anion exchange column; discoloring the effluent, condensing the effluent in vacuum, carrying out isoelectric point crystallization, washing the crude crystals by an organic solvent, and finally drying. According to the provided process, the using amount of exchange resin can be reduced prominently, solved is the problem of converting glutamine between a strong alkaline environment and a strong acidic environment, the production cost is largely reduced, the total extraction rate of glutamine can reach 70% or so, and the process is suitable for industrial production.

Description

technical field [0001] The invention relates to a process for extracting glutamine from fermentation liquid, belonging to the technical field of amino acid preparation for medicine and health care. Background technique [0002] L-glutamine (Gin) is the Y-carboxyl amidate of L-glutamic acid (Glu), and is one of the 20 basic amino acids that constitute proteins. Its chemical formula is H.NC0 CH 2 ·CH 2 ·C_ 2 ) C00H, molecular weight 146.15, decomposition temperature 184-185°C, isoelectric point 5.65, belongs to neutral amino acid. In recent years, medical discoveries have shown that glutamine is a conditionally essential amino acid, which plays a pivotal role in biological metabolism, and a variety of diseases will be caused when it is lacking. [0003] The main producers of glutamine by fermentation are Japan and South Korea. It is estimated that the annual output of glutamine in the world has reached about 4,000 tons, and it is showing an increasing trend. The research...

Claims

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Application Information

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IPC IPC(8): C07C237/06C07C231/24
Inventor 刘振宇
Owner 刘振宇
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