Bacillus methylotrophicus strain NKG-1 and plant growth promoting method

A methylotrophic and Bacillus technology, applied in botany equipment and methods, microbial-based methods, plant growth regulators, etc., can solve problems such as endangering human health, destroying ecological balance, and environmental pollution, and achieve no pesticides residual effect

Active Publication Date: 2016-04-27
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term large-scale application of chemical fertilizers leads to residues and environmental pollution, which eventually endangers human health and destroys ecological balance.

Method used

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  • Bacillus methylotrophicus strain NKG-1 and plant growth promoting method
  • Bacillus methylotrophicus strain NKG-1 and plant growth promoting method
  • Bacillus methylotrophicus strain NKG-1 and plant growth promoting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1: tomato growth-promoting test method

[0075] Such as Figure 4 , Figure 5 One embodiment of the invention is shown. The left side of the figure is the CK group, and the right side is the root irrigation group of strain NKG-1. It is also possible to adopt the method of preparing the bacterial solution into a wettable powder and then diluting it to obtain a liquid, and using the liquid to irrigate the roots of the tomato.

[0076] The test was planted in plug trays and carried out in nutrient soil. The ratio of nutrient soil and vermiculite was 9:1 to configure the matrix and place it in the plug trays. Sprinkle 2-3 tomato seeds in each hole, cover the seeds with a small amount of nutrient soil, irrigate with an appropriate amount of water, and cover with plastic wrap, and remove the plastic wrap when the seedlings emerge from the soil and grow 2 cotyledons. The test was divided into two groups, and the test group used methylotrophic bacillus bacteria s...

Embodiment 2

[0080] Embodiment 2: Chinese cabbage growth-promoting test method

[0081] Such as Figure 6 , Figure 7 One embodiment of the invention is shown. The left side of the figure is the CK group, and the right side is the strain NKG-1 bacterial solution soaking group. It is also possible to prepare the bacterium liquid into a wettable powder and then dilute it to obtain a liquid, and use the liquid to soak the pak choy seeds.

[0082] Firstly, the methylotrophic bacillus liquid (concentration of 1×10 7 cfu / mL) and clear water for 24 hours, and then sow them in hole trays respectively, with 2-3 seeds per hole, 20 seedlings for each treatment, and repeat 3 times. The plug tray contains a matrix of nutrient soil and vermiculite in a ratio of 9:1. After sowing, cover it with a small amount of nutrient soil, water it with an appropriate amount of water, and cover it with plastic wrap. In the environment of 25 ° C in the greenhouse and a relative humidity of 60% Cultivate, and remo...

Embodiment 3

[0087] Embodiment 3: Capsicum growth-promoting test method

[0088] Such as Figure 8 , Figure 9 One embodiment of the invention is shown. The left side of the figure is the CK group, and the right side is the root irrigation group of strain NKG-1. It is also possible to adopt the method of preparing the bacterial solution into a wettable powder and then diluting it to obtain a liquid, and utilizing the liquid to irrigate the capsicum roots.

[0089] The test was planted in plug trays and carried out in nutrient soil. The ratio of nutrient soil and vermiculite was 9:1 to configure the matrix and place it in the plug trays. Sprinkle 2-3 pepper seeds in each hole, cover the seeds with a small amount of nutrient soil, irrigate with a proper amount of water, and cover with plastic wrap, and remove the plastic wrap when the seedlings emerge from the soil and grow 2 cotyledons. The test was divided into two groups, and the test group used methylotrophic bacillus bacteria soluti...

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Abstract

The invention discloses a bacillus methylotrophicus strain NKG-1. The bacillus methylotrophicus strain NKG-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), wherein the preservation number is CGMCC 12055, and the preservation date is Jan 15, 2016. With a plant growth promoting method implemented in the strain, an obvious growth promoting effect is achieved, and therefore, the application prospects are good.

Description

technical field [0001] The present invention relates to a kind of methylotrophic bacillus, also relate to the plant growth promoting method that utilizes this bacterial strain to implement, specifically relate to a kind of methylotrophic bacillus (Bacillus methylotrophicus strain NKG-1), and its effect on tomato, Chinese cabbage, pepper Promoting method. Background technique [0002] In order to promote the growth of plants, such as various crops, fertilizers are usually applied at various growth stages of plants at present, mainly chemical fertilizers, which have the characteristics of convenient fertilization and quick results. However, long-term large-scale application of chemical fertilizers leads to residues and environmental pollution, which eventually endangers human health and destroys ecological balance. Because of its advantages on the environment, ecology, human health, safety, and non-toxicity, biopromoting has been widely valued by countries all over the world ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01G7/06A01C1/00C12R1/07
CPCA01C1/00A01G7/06A01N63/00C12N1/205C12R2001/07
Inventor 葛蓓孛张克诚刘炳花刘艳赵文珺
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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