Proliferation medium for increasing saffron crocus bud proliferation rate

A kind of proliferation medium, the technology of saffron, applied in the field of proliferation medium for improving the bud proliferation rate of saffron, can solve the problems such as low bud proliferation rate, unstable callus character, difficult to meet rapid propagation, etc., to promote bud proliferation, improve The effect of bud growth rate

Inactive Publication Date: 2016-05-04
FOSHAN JINLANLING EDUCATION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the rapid propagation of saffron mainly adopts tissue culture, and the tissue culture of saffron can use bulbs to induce callus, and then induce buds after callus proliferation,...

Method used

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  • Proliferation medium for increasing saffron crocus bud proliferation rate
  • Proliferation medium for increasing saffron crocus bud proliferation rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment one: prepare a kind of proliferation medium that improves saffron bud proliferation rate.

[0013] According to the MS medium formula shown in Table 1 and the proliferation medium formula of improving the saffron bud proliferation rate shown in Table 2, prepare the proliferation medium for improving the saffron bud proliferation rate of the present invention.

[0014] Use a balance to weigh the components in the MS medium, 6-BA (6-benzyl adenine), P-CPA (p-chlorophenoxyacetic acid), coconut water, agar powder and sucrose, and place them in the Erlenmeyer flask. Add an appropriate amount of purified water, stir to dissolve, set the volume to 1000mL, and adjust the pH value to 6.0 with HCL or KOH. Seal the triangular flask, place it in an autoclave, and sterilize at 121°C for 21 minutes. Then, in an ultra-clean workbench, divide the proliferation medium that increases the proliferation rate of saffron buds into tissue culture flasks, cool naturally, and place t...

Embodiment 2

[0019] Embodiment two: the test of the proliferation medium that improves the saffron bud proliferation rate of the present invention.

[0020] According to the medium preparation method described in Example 1, prepare the proliferation medium that improves the saffron bud proliferation rate, and set aside.

[0021] Select saffron buds without pollution, normal growth, browning and vitrification from the induction medium, take out the saffron buds in the ultra-clean bench, separate the buds into single buds with a sterile scalpel, and inoculate them into the above prepared In the proliferation medium that improves the proliferation rate of saffron buds, each bud is spaced 1.5 centimeters apart from each other.

[0022] After the inoculation, the saffron buds and the medium were placed in an artificial climate box for cultivation. The cultivation conditions were as follows: light / dark time: 14 / 10 hours, light intensity was 2000lux, and the cultivation temperature was 23°C. Afte...

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Abstract

The invention provides a proliferation medium for increasing the saffron crocus bud proliferation rate, and belongs to the technical field of plant tissue culture. An MS culture medium is used as a basic culture medium, and 6-BA(6-benzyladenine), P-CPA(p-chlorophenoxyacetic acid), coconut water, agar powder and saccharose are added. The proliferation medium for increasing the saffron crocus bud proliferation rate has the advantage of being high in bud proliferation rate.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a proliferation medium for improving the proliferation rate of saffron buds. Background technique [0002] Saffron is a kind of precious Chinese herbal medicine. Because it is only used as medicine on the stigma, the yield is extremely low. At the same time, because of its extremely limited resources, but its wide range of uses, it is expensive. It has always been known as "plant gold", so the rapid propagation of saffron Research is extremely important. [0003] At present, the rapid propagation of saffron mainly adopts tissue culture, and the tissue culture of saffron can use bulbs to induce callus, and then induce buds after callus proliferation, but the properties of callus are unstable; it can also be directly induced into buds by bulbs, but the buds The proliferation rate is low, and it is difficult to meet the requirements of rapid reproduction. Contents of...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 何志铿
Owner FOSHAN JINLANLING EDUCATION TECH
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