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Nano-carbon crystal drug carrier for target identification of tumor cells and preparation method of drug carrier

A nano-drug carrier and tumor cell technology, applied in anti-tumor drugs, nanotechnology for materials and surface science, nanotechnology, etc., can solve problems such as difficult long-term effective attachment, lack of universal applicability, and low modification efficiency , to achieve the effect of increasing target recognition rate, weak toxicity and good biocompatibility

Inactive Publication Date: 2016-05-04
ZHENGZHOU ARTIFICIAL DIAMOND & PROD ENG TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing nano-drug carriers only consider the modification of active transport proteins when modifying characteristic drug molecules. This modification is inefficient and difficult to effectively attach to the surface of nano-drug carriers for a long time, and this modification is not universally applicable. sex

Method used

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  • Nano-carbon crystal drug carrier for target identification of tumor cells and preparation method of drug carrier
  • Nano-carbon crystal drug carrier for target identification of tumor cells and preparation method of drug carrier
  • Nano-carbon crystal drug carrier for target identification of tumor cells and preparation method of drug carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A preparation process of nano carbon crystal, and its process flow frame Picture like Figure 4 As shown, the steps are:

[0045] (1) Preliminary crushing: First, feed the diamond raw material into the crushing cavity of the mechanical crusher, and crush it by the action of the high-speed rotating hammer inside the crusher. The crushed material passes through screens of different meshes, larger than the diameter of the screen. The material is left in the crusher and re-crushed. The particles filtered by the finest screen in the lower layer of the crusher are collected by the screen plate (the screen mesh is not less than 32 meshes), and the material filtered by other mesh screens can be regarded as Coarse materials are sold or used to prepare materials of other particle sizes; particles with 32 meshes or more are used for preliminary particle size separation by a multi-layer vibrating sieve machine. The vibrating time is generally controlled within 30min~3h, and the number...

Embodiment 2

[0053] The nano-medicine carrier material for targeting and recognizing tumor cells includes nano-carbon crystals, the C atoms on the surface of the nano-carbon crystals are sequentially connected to adriamycin and transferrin. The preparation method of nano carbon crystals is the same as in Example 1.

[0054] The preparation method of the nano-medicine carrier material includes the following steps:

[0055] 1) Nano-carbon crystal pretreatment: place the nano-carbon crystal in a concentrated H with a mass ratio of 3:1 2 SO 4 / Concentrated HNO 3 In the mixed solution, heated to reflux at 88~92℃ for 36h, centrifuged and precipitated at 1500rpm; take the precipitate and wash it with distilled water to pH 4.0~5.0, and then use 0.2mol·L -1 Wash with NaOH and disperse ultrasonically for 1h. This process is repeated three times, and then 0.24mol·L -1 Wash with HCl and disperse ultrasonically for 1 hour. Repeat this process three times, then wash and dry with deionized water;

[0056] 2) Pr...

Embodiment 3

[0063] The nano-medicine carrier material for targeting and recognizing tumor cells includes nano-carbon crystals, the C atoms on the surface of the nano-carbon crystals are sequentially connected to adriamycin and transferrin. The preparation method of nano carbon crystals is the same as in Example 1.

[0064] The preparation method of the nano-medicine carrier material includes the following steps:

[0065] 1) Pretreatment of nano-carbon crystals: place the nano-carbon crystals in a concentrated H with a mass ratio of 2:1 2 SO 4 / Concentrated HNO 3 In the mixed solution, heated to reflux at 88~92℃ for 24h, centrifuged and precipitated, the speed of centrifugation was 1000rpm; take the precipitate and wash it with distilled water to pH 4.0~5.0, and then use 0.1molL -1 Wash with NaOH, and ultrasonically disperse for 2h, this process is repeated 4 times, then 0.1mol·L -1 Wash with HCl, and ultrasonically disperse for 2h, repeat this process 4 times, then wash and dry with deionized w...

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Abstract

The invention discloses a nano-carbon crystal drug carrier material for target identification of tumor cells and a preparation method of the drug carrier material. The drug carrier material consists of nano-carbon crystals, and adriamycin and transferrin are sequentially linked to C atoms on the surfaces of the nano-carbon crystals. The preparation method comprises the following steps: 1) preprocessing the nano-carbon crystals; 2) preparing a transferrin-adriamycin physical composite solution; and 3) assembling the nano-carbon crystals and the transferrin-adriamycin physical composite solution, so that the drug carrier material is obtained. The obtained nano-drug carrier material of the invention is strong in targeting property and is high in modifying rate; and moreover, active transport protein can be modified on the surface of the drug carrier material in an immobilized mode, and the drug carrier material has the advantage of universal applicability.

Description

technical field [0001] The invention relates to a nano drug carrier material for targeting and recognizing tumor cells and a preparation method thereof. Background technique [0002] At present, antineoplastic drugs mainly include cytotoxic drugs, hormones and antihormonal drugs. Cytotoxic drugs mainly act on DNA and RNA to organize tumor cell proliferation and life metabolism; hormone and antihormonal drugs mainly act on living organisms to regulate hormone levels to kill or inhibit tumor cell proliferation and life activities. These anti-tumor drugs act on normal cells as well as tumor cells, and the damage to normal tissues of the body is no less than the damage to tumor tissue cells. Drug spillage outside blood vessels can also cause tissue ulceration and necrosis. In addition, traditional chemotherapeutic anti-tumor drugs have non-selective and guiding effects, and the transportation process in the body is complicated and the proportion of drug molecules reaching the ...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K47/04A61K31/704A61K47/42A61P35/00C01B31/02B82Y30/00B82Y40/00
CPCA61K31/704A61K47/02A61K47/42C01P2004/64C01P2006/80
Inventor 何本锋武艳强刘君丽郭留希赵清国刘永奇杨晋中张建华邵静茹穆小娜
Owner ZHENGZHOU ARTIFICIAL DIAMOND & PROD ENG TECH RES CENT
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