Preparation method of ATP (adenosine triphosphate) near infrared fluorescent probe
A technology of adenosine triphosphate and fluorescent probes, applied in the field of small molecule fluorescent probes, can solve the problems of decreased fluorescence intensity, low sensitivity of probes, and sensitivity to scattered light, etc., to reduce external interference, fast and convenient analysis, and simple synthesis steps Effect
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[0029] Referring to the drawings, the present invention is a preparation method of adenosine triphosphate near-infrared fluorescent probe, and its steps are:
[0030] S1. Dissolve the squaraine dye in an organic solution to obtain an organic solution of squaraine;
[0031] S2. Dissolve N,N-bis(2-aminoethyl)-1,2-ethylenediamine in an organic solution to obtain N,N-bis(2-aminoethyl)-1,2-ethylenediamine Organic solution
[0032] S3. Add the squaraine organic solution dropwise to the N,N-bis(2-aminoethyl)-1,2-ethylenediamine organic solution, stir at room temperature for 2 hours, then remove the organic solution and pass it through the column Chromatographic separation and purification to obtain near-infrared fluorescent probe of adenosine triphosphate.
[0033] The organic solution includes a dichloromethane solution, especially anhydrous dichloromethane.
[0034] The concentration of the squaraine organic solution was 4.19 mM.
[0035] The concentration of the N,N-bis(2-aminoethyl)-1,2-e...
Example Embodiment
[0043] Example 1: Adenosine triphosphate near-infrared fluorescent probe.
[0044] This embodiment includes the following steps:
[0045] S1. Dissolve 250 mg of squaraine dye in 100 mL of anhydrous methylene chloride to obtain an organic solution of squaraine;
[0046] S2. Dissolve 360μL of N,N-bis(2-aminoethyl)-1,2-ethylenediamine in anhydrous dichloromethane to obtain N,N-bis(2-aminoethyl)-1, 2-ethylenediamine organic solution;
[0047] S3. Add the squaraine organic solution dropwise to the N,N-bis(2-aminoethyl)-1,2-ethylenediamine organic solution, stir vigorously at room temperature for 2 hours, and then remove the anhydrous dichloride Methane is used to obtain crude near-infrared dyes, which are then separated and purified by column chromatography to obtain a blue-violet solid powdery adenosine triphosphate near-infrared fluorescent probe.
[0048] The molecular structure of the obtained adenosine triphosphate near-infrared fluorescent probe is as follows:
[0049]
[0050] The st...
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