A kind of compound bacterial agent and its application in the alcoholization of tobacco leaves

A technology of compound bacterial agent and tobacco leaf alcohol, applied in the direction of application, bacteria, tobacco, etc., can solve the problem of limited improvement effect, achieve the effect of increasing the variety and improving the quality of alcoholization

Active Publication Date: 2021-05-28
CHINA TOBACCO GUIZHOU IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the improvement effect of the above methods is limited

Method used

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  • A kind of compound bacterial agent and its application in the alcoholization of tobacco leaves
  • A kind of compound bacterial agent and its application in the alcoholization of tobacco leaves
  • A kind of compound bacterial agent and its application in the alcoholization of tobacco leaves

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Effect test

Embodiment 1

[0036] The preparation of embodiment 1 composite bacterial agent

[0037] 1.1 Preparation of medium

[0038] 1) Preparation of PDA liquid medium: weigh 200g potatoes, wash, peel and chop, add water 1000mL ddH 2 O Boil for 30 minutes, filter through four layers of gauze, add 20g of glucose, fully dissolve, sterilize at 121°C for 30 minutes and cool for later use.

[0039] 2) Preparation of wheat culture medium: Wheat was crushed into half and half of wheat grains and half of wheat flour, 100 g in total, added with 50% water, sterilized at 121°C for 30 minutes, cooled and dispersed for later use.

[0040] 1.2 Preparation of composite bacterial agent

[0041] 1) Isolation of Bacillus subtilis and Bacillus licheniformis

[0042] Inoculate the black part of Moutai koji on the PDA plate medium, culture at 50°C for 24 hours, pick a single colony for purification and culture, add the isolated purified strain to the wheat medium, and follow the temperature of 37°C, 46°C, The temper...

Embodiment 2

[0046] Embodiment 2 Alcoholization and fermentation treatment of tobacco leaves

[0047] Select A3C2 (middle leaves) in the Qianxinan area of ​​Guizhou Province to test, first adopt the simultaneous distillation and extraction method to pretreat the tobacco leaves, and spray the composite bacterial agent prepared in the above-mentioned embodiment 1 with an inoculation amount of 1% (1mL bacterial liquid / kg tobacco leaves) On the tobacco leaves, the alcoholic fermentation treatment was carried out at a temperature of 40° C. for 25 days.

[0048] At the same time, the same amount of water was sprayed as a control example.

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Abstract

The invention relates to a composite microbial agent, which is prepared by respectively culturing bacillus subtilis, bacillus licheniformis and bacillus amylase and mixing them in proportion. The Bacillus subtilis and Bacillus licheniformis are isolated from Maotai distiller's yeast. The present invention links the functional development of aroma-causing microorganisms of Moutai wine with the improvement of tobacco quality, and applies the fermentation products of Moutai koji microorganisms to tobacco leaf fermentation for the first time. Through the activities of these aroma-causing microorganisms or the addition of their metabolites, the fermentation of tobacco leaves can be increased The types of medium and high-quality microorganisms increase the types of aroma substances, which has an effect similar to that of adding Moutai to enhance the aroma, thereby improving the quality of tobacco leaves aging.

Description

technical field [0001] The invention relates to a composite bacterial agent and its application in tobacco leaf alcoholization, belonging to the technical field of tobacco leaf aroma enhancement treatment. Background technique [0002] In the fermentation stage of tobacco leaves, adding specific microorganisms can effectively shorten the fermentation time of tobacco leaves, regulate the content of main chemical components in tobacco leaves (reduce the content of nicotine in tobacco leaves, reduce the protein of tobacco leaves, reduce the content of amino acids, and reduce TSNA), and increase the aroma precursors of tobacco leaves. substance content. [0003] Many research results have shown that by improving the microbial fermentation conditions of tobacco leaves or adding various substances, the internal quality of tobacco leaves can be significantly improved, and the miscellaneous gas and irritation of tobacco leaves can be reduced. As reported by Seaaich et al., spraying...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B15/28C12R1/10C12R1/125C12R1/07
Inventor 王芳连宾张庆明王玉平丁根胜姬厚伟
Owner CHINA TOBACCO GUIZHOU IND
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