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Composition capable of stimulating expansion of T cells

A composition and cell technology, applied in the direction of animal cells, vertebrate cells, cell culture active agents, etc., can solve the problems of short lifespan and achieve the effects of prolonging the aging time, prolonging the half-life, and increasing the amplification factor

Inactive Publication Date: 2016-05-04
SUN YAT SEN UNIV
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  • Claims
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Problems solved by technology

[0006] On the other hand, CD8 + Effector T cells (EffectTcell, Teff) have a short lifespan and cannot maintain a long-term immune response in the body

Method used

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  • Composition capable of stimulating expansion of T cells
  • Composition capable of stimulating expansion of T cells
  • Composition capable of stimulating expansion of T cells

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Embodiment Construction

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. The peripheral blood of patients with HIV-1 infection was provided by Guangzhou Eighth People's Hospital. Unless otherwise specified, the reagents, equipment and methods used in the present invention are commercially available reagents, equipment and routinely used methods in this technical field.

[0034] Antigen-specific CD8 for HIV-1 infected patients + T cell-specific activation and expansion

[0035] Over the past few decades, antigen-specific CD8 + T cell structure and function have been studied in great detail. Amplified HIV-1 antigen-specific CD8 of the present invention + The memory T cell expansion process is as follows: figure 1 shown.

[0036] Isolate peripheral blood mononuclear cells (Peripheral blood mononuclear cells, PBMC) from the peripheral blood of HIV-1 infected patients (dilute 10 mL of peripheral blood to 25...

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Abstract

The invention discloses a composition capable of stimulating expansion of T cells. Active factors of the composition comprise an Anti-CD3 antibody, an Anti-CD28 antibody, IL-2, IL-7 and IL-21. Through an HIV-1 specificity polypeptide combination and a multiple cell factor combination, in-vitro expansion of the antigen-specific memory T cells (TSCM and TCM) of an HIV-1 infected person can be effectively and continuously stimulated, and meanwhile the proportion of the expanded CD8<+> memory T cells, especially the CD8<+> TSCM is increased. The half-life period of adoptive immunotherapy reinjection cells can be prolonged, therefore, the immune surveillance achieving ageing of the adoptive immunotherapy reinjection cells is prolonged, and the potential of controlling HIV-1 latent infection for a long term is achieved. According to the method, the advantages that operation is simple, and materials are easy to obtain are achieved, and good technical support is supplied to wide development of anti-infection adoptive immunotherapy.

Description

technical field [0001] The invention relates to a composition capable of stimulating T cell expansion and a culture method for expanding CD8+T cells using the composition. Background technique [0002] AIDS is an important disease that threatens the safety of human life, and human immunodeficiency virus is the main pathogenic factor of AIDS. The important treatment for AIDS at this stage is cocktail therapy, and patients take drugs for a long time to control the virus and the course of the disease. However, the cocktail therapy cannot completely clear the virus. Due to the latent infection of HIV-1, once the drug is stopped, the virus will rebound quickly. On the other hand, HIV-1 will gradually develop drug resistance through high-frequency mutations. And taking medicine for a long time is also a heavy burden to the patient itself. [0003] Studies have shown that HIV antigen-specific CD8 + T cells play a very important role in the control and clearance of the virus res...

Claims

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Application Information

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IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N2501/998C12N2501/2321C12N2501/2307C12N2501/2302
Inventor 张辉刘炳峰
Owner SUN YAT SEN UNIV
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