Composition capable of stimulating expansion of T cells
A composition and cell technology, applied in the direction of animal cells, vertebrate cells, cell culture active agents, etc., can solve the problems of short lifespan and achieve the effects of prolonging the aging time, prolonging the half-life, and increasing the amplification factor
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[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. The peripheral blood of patients with HIV-1 infection was provided by Guangzhou Eighth People's Hospital. Unless otherwise specified, the reagents, equipment and methods used in the present invention are commercially available reagents, equipment and routinely used methods in this technical field.
[0034] Antigen-specific CD8 for HIV-1 infected patients + T cell-specific activation and expansion
[0035] Over the past few decades, antigen-specific CD8 + T cell structure and function have been studied in great detail. Amplified HIV-1 antigen-specific CD8 of the present invention + The memory T cell expansion process is as follows: figure 1 shown.
[0036] Isolate peripheral blood mononuclear cells (Peripheral blood mononuclear cells, PBMC) from the peripheral blood of HIV-1 infected patients (dilute 10 mL of peripheral blood to 25...
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