Microfluidic liquid-liquid extraction-liquid-liquid waveguide integrated detection system and detection method

Abstract

The invention relates to a microfluidic detection system integrating liquid-liquid extraction and liquid-liquid waveguide and a detecting method. The microfluidic detection system comprises a microfluidic chip, a liquid-liquid extraction system and a liquid-liquid waveguide detecting system. According to the microfluidic detection system, an inlet end of an extracting path is communicated with inlets (I to V) for respectively injecting an ionic liquid, a sample-containing inorganic salt solution and an inorganic slat solution, a spiral part outlet end of the extracting path is communicated with an inlet end of a linear type detecting path, both ends of the detecting path are respectively provided with incident / receiving optical fibers and incident end / receiving end collimating lens. The microfluidic detection system adopts the ionic liquid as a liquid core and adopts the inorganic salt solution as a cover layer. Extracted multi-phase layer streams directly flow into the detecting path, the liquid-liquid waveguide absorbancy detecting system is constructed by utilization of total reflection phenomena on a liquid-liquid interface, and absorbancy of a sample in the liquid core is measured by adopting an absorbancy detecting method, thus increasing the number of times of enrichment of extracted compounds, increasing the effective optical length and avoiding sample contamination.

Description

technical field [0001] The invention belongs to the field of chemical detection, and specifically relates to a detection system and a detection method that adopts microfluidic technology and integrates liquid-liquid extraction and liquid-liquid waveguide detection. Background technique [0002] Microfluidic technology has been widely used in the field of detection and analysis due to the advantages of less sample consumption. The currently established microfluidic chip liquid-liquid extraction method has the advantages of only microliters, picoliters or even nanoliters of extraction reagents and sample consumption, and the extraction time is only a few minutes or even seconds, and has been widely used. However, the existing problem is that the established microfluidic chip liquid-liquid extraction method usually separates the extract from the microchannel and then proceeds to the next step of detection, that is, the analysis and detection are carried out outside the chip, an...

AI Technical Summary

Problems solved by technology

However, the existing problem is that the established microfluidic chip liquid-liquid extraction method usually separates the extract from the microchannel and then proceeds to the next step of detection, that is, the analysis and detection are carried out outside the chip, and the extract needs to be introduced It still needs to consume a lot of samples in conventional instruments, and the samples are easy to be polluted during the transfer process, which greatly reduces the advantages of microfluidic technology and does not highlight the advantages of microextraction

However, due to the small volume and short absorption path of the microfluidic chip channel detection area, the detection sensitivity is low, which seriously restricts the application of absorption photometric detection technology in microfluidic analysis.

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