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Scutellaria barbata polysaccharide antitumor mechanism detection research method

A technology of bark lotus polysaccharide and anti-tumor mechanism, which is applied in the directions of anti-tumor drugs, pharmaceutical formulations, organic active ingredients, etc. Problems such as the lack of systematic research reports on the effective chemical sites of tumors

Inactive Publication Date: 2016-05-11
HUAIAN COLLEGE OF INFORMATION TECH
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Problems solved by technology

However, in my country, most of the treatment of some tumor diseases with Scutellaria barbata is oral crude preparations. So far, it is recognized that Scutellaria barbata has anti-tumor effects, but the effective chemical components and effective chemical parts of Scutellaria barbata are lacking systematically. However, there have been no research reports on the anti-tumor and cellular immune mechanisms of Scutellaria barbata polysaccharides in vivo

Method used

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  • Scutellaria barbata polysaccharide antitumor mechanism detection research method
  • Scutellaria barbata polysaccharide antitumor mechanism detection research method
  • Scutellaria barbata polysaccharide antitumor mechanism detection research method

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Embodiment Construction

[0059] Such as Figures 1 to 3 As shown, a method for detecting the anti-tumor mechanism of Scutellaria barbata polysaccharides, the detection steps include:

[0060] a) In vivo tumor inhibition experiment;

[0061] b) Determination of the total number of T cells in peripheral blood of tumor-bearing mice by ANAE method;

[0062] c) Determination of T cell subsets and T cell surface protein—CD28 in the peripheral blood of tumor-bearing mice by flow cytometry;

[0063] d) Detection of IL-2 and IFN-γ levels in peripheral blood serum of tumor-bearing mice by ELISA method;

[0064] Wherein, the tumor suppression experiment in vivo is carried out according to the following steps:

[0065] 1) The mice were randomly divided into 6 groups, 10 in each group, respectively negative control group (normal saline); positive control group (astragalus polysaccharide, cyclophosphamide); Scutellaria barbata polysaccharide low-dose group, middle-dose group and high-dose group Group;

[0066]...

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Abstract

The present invention discloses a scutellaria barbata polysaccharide antitumor mechanism detection research method which comprises the following detecting steps: an in-vivo tumor suppression experiment; ANAE method determination of the total number of T cells in peripheral blood of tumor-bearing mice; flow cytometry determination of T cell subsets and T cell surface protein-CD28 in the peripheral blood of the tumor-bearing mice; and determination of content of IL-2 and IFN-gamma in serum of the peripheral blood of the tumor-bearing mice; the scutellaria barbata polysaccharide in-vivo antitumor and cell immune function influence detection research method discloses a major role of scutellaria barbata polysaccharide in tumor treatment, and meanwhile the study on body cell immune mechanism of plant polysaccharides can be promoted.

Description

technical field [0001] The invention belongs to the field of anti-tumor pharmacology, in particular to a research method for detecting the anti-tumor mechanism of Scutellaria barbata polysaccharides. Background technique [0002] Tumor is one of the main diseases that endanger human health, and malignant tumors seriously threaten human life and health. With the aging of the population in China and the existence of problems such as smoking and infection, the situation of cancer prevention and treatment is extremely severe. Existing studies have shown that plant polysaccharides, as an important class of biological regulators, have good application prospects in anti-tumor, immune regulation and anti-aging, and have less toxic and side effects. Therefore, finding high-efficiency and low-toxic antitumor drugs in plant polysaccharides is one of the important contents of current tumor research, which has certain theoretical and practical significance. [0003] Banzhilian was firs...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P35/00
Inventor 杨姗姗张秀娟陆敏
Owner HUAIAN COLLEGE OF INFORMATION TECH
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