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Corydalis edulis extract, pharmaceutical composition containing same and uses thereof

A technology of extracts and compositions, applied in the field of preparation of medicines for diseases caused by disorders of glucose and lipid metabolism, can solve the literature reports on the improvement effect of Corydalis extracts on glucose and lipid metabolism disorders, and Corydalis extracts that have not been seen , There is no problem such as the effect of Corydalis extract on promoting insulin secretion, and it can improve the injection glucose tolerance, improve glucose tolerance, and improve angina pectoris.

Active Publication Date: 2016-05-11
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] So far, there are no studies on Corydalis extract (KDD) in reducing blood sugar, blood lipids, anti-atherosclerosis, and improving fatty liver, and there are no literature reports on the effect of Corydalis extract on improving glucose and lipid metabolism disorders; There are no literature reports on the insulin-stimulating effect of Viola extract

Method used

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  • Corydalis edulis extract, pharmaceutical composition containing same and uses thereof
  • Corydalis edulis extract, pharmaceutical composition containing same and uses thereof
  • Corydalis edulis extract, pharmaceutical composition containing same and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: the preparation of total alkaloid extract of Viola corydalis of the present invention

[0054] (1) Take 200g of the plant Viola corydalis and pulverize it, add 2000mL of 1% Na 2 CO 3 The 70% volume ratio ethanol solution was extracted three times at 80°C, and the three extracts were combined to obtain an alkaline ethanol extract;

[0055] (2) The extract obtained in step (1) is concentrated under reduced pressure to obtain 45 g of extract;

[0056] (3) add the hydrochloric acid solution of the 1% volume ratio of 150mL to the medicinal extract that step (2) obtains and dissolve, filter, obtain the acid water liquid, then add the sodium hydroxide solution of 10% volume ratio in the acid water liquid to adjust the pH to 10, obtain alkali treatment liquid;

[0057] (4) Add dichloromethane to the alkali-treated solution obtained in step (3) and extract 4 times, 500 mL each time, combine the 4 extracts, recover the dichloromethane under reduced pressure, an...

Embodiment 2

[0058] Example 2: Experimental Research on Insulin Secretion Stimulation of Corydalis Corydalis Extract of the Present Invention

[0059] 1. Experimental materials:

[0060] 1. Drugs: (1) Test drug: Corydalis purpurea extract of the present invention; (2) positive control drug: atorvastatin (Pfizer Pharmaceutical Co., Ltd., H20051407), gliclazide (Tianjin Huajin Pharmaceutical Co., Ltd. Company, H10910053).

[0061] 2. Animals: SPF grade C57BL / 6ApoE- / - mice, 6-8 weeks old, weighing 19-23g, male; provided by the Animal Center of Peking University Health Science Center.

[0062] 3. Reagents and instruments: Glucose (Beijing Baierdi Biotechnology Co., Ltd.); Insulin injection (NovoNordisk, Denmark); Mouse insulin ELISA quantitative detection kit (Mercodia, Sweden); blood glucose meter and blood glucose test paper (Roche Vitality, GU type, Germany); multi-functional microplate reader (TECAN, Switzerland, M1000 type); centrifuge (Eppendorf, USA, 5424R type); electronic balance...

Embodiment 3

[0094] Example 3: Experimental Research on Insulin Secretion Stimulation of Corydalis Corydalis Extract of the Present Invention

[0095] 1. Experimental materials:

[0096] 1. Cells: Hamster HIT-T15 cells

[0097] 2. Test drug: Corydalis purpurea extract of the present invention

[0098] 3. Reagents and instruments: KRB buffer (Krebs-Ringer Bicarbonate Buffer); RPMI1640 cell culture medium and fetal bovine serum (Beijing Baierdi Biotechnology Co., Ltd.); Gliclazide (China Institute for Food and Drug Control); cell culture box (SANYO company, MCO-18AIC); ultra-clean bench (ESCO company, OptiMair); centrifuge (Eppendorf, 5424R type); multifunctional microplate reader (Switzerland TECAN company, M1000 type); mouse insulin ELISA quantitative detection reagent box (Mercodia, Sweden).

[0099] 2. Experimental method:

[0100] HIT-T15 cells were cultured with RPMI1640 complete medium containing 10% (volume percentage) fetal bovine serum, and when they grew logarithmically, the...

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Abstract

The invention provides Corydalis edulis extract, prepared by a method comprising the following steps: (1), after crushing a Corydalis edulis plant, and extracting with alkaline ethyl alcohol or methyl alcohol to obtain an extract; (2), subjecting the extract obtained in step (1) to normal pressure or vacuum concentration to obtain concrete, or spray drying to obtain powder; (3), adding an acid solution into the concrete or powder obtained in step (2), filtering to obtain an acid aqueous solution, adding an alkaline solution to the acid aqueous solution to obtain an alkali treated solution; (4), adding an organic solvent to the alkali treated solution obtained in step (3), vacuum recycling the organic solvent after extracting to obtain Corydalis edulis total alkaloids crude extract. The Corydalis edulis extract or a pharmaceutical composition containing the extract can be used to prepare pharmaceuticals for preventing and / or treating diseases caused by glucose and lipid metabolism disorder.

Description

technical field [0001] The invention belongs to the technical field of pharmacy. Specifically, the present invention relates to the use of the plant extract extracted from Corydalis violet plant in the preparation of medicines for diseases caused by disorders of glucose and lipid metabolism. Background technique [0002] Insulin resistance (Insulin Resistance, IR) refers to the reduced responsiveness of the body to the physiological effects of insulin, which is manifested in the decreased sensitivity of peripheral tissues to insulin and the impairment of glucose utilization. The main reason is that due to the reduction of high-calorie diet or physical activity, as well as other aging factors, stress state, genetic factors, etc., the response of the target tissue of the body to insulin is weakened, and the ability of insulin to maintain blood sugar is reduced. Fat and protein and other substance metabolism disorders and energy metabolism disorders (Liu Liping. Establishment ...

Claims

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Application Information

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IPC IPC(8): A61K36/66A61P3/10A61P3/06A61P5/50A61P9/10A61P1/18A61P1/16A61P3/12A61P9/14
CPCA61K36/66A61K2236/333A61K2236/51
Inventor 屠鹏飞郑姣李军伦琦星刘炳琳顾小盼赵云芳
Owner BEIJING UNIV OF CHINESE MEDICINE
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