13 results about "Corydalis edulis" patented technology

The invention discloses an establishment method of an HPLC (high performance liquid chromatography) characteristic chromatogram of granules for treating common cold and clearing heat and a component content test thereof, and belongs to the technical field of pharmaceutical analys.According to the research, the HPLC characteristic chromatogram of the granules for treating common cold and clearing heat is established under the same chromatographic condition by adopting a high performance liquid chromatography, and the overall internal quality in a preparation is comprehensively reflected; the method for simultaneously determining the content of amygdalin, puerarin, cimicifugin and corydalis bungeana is established, methodology verifies that the method is simple, convenient and feasible, has good stability and repeatability, and can be used for detection in production process monitoring and quality evaluation of the cold and heat clearing granules to ensure that the quality of the cold and heat clearing granules meets the standard. The scheme not only scientifically restores the overall pharmacodynamic material basis of the compound preparation, but also provides a new generalizable normal form for quality control of traditional Chinese medicines with the advantages of low cost and high efficiency, and has milestone significance for promoting industry standardization and internationalization.

The present invention relates to a method for cultivating transgenic plants having increased anthocyanin content and enhanced tolerance to biotic and abiotic stress environments. The method comprises transferring a bHLH35 gene into recipient plants using a genetic engineering transformation method, to obtain transgenic plants having increased anthocyanin content and tolerance to biotic and abiotic stress environments. The bHLH35 gene may be selected from a ChbHLH35 gene from Corydalis hemidicentra or an AtbHLH35 gene from Arabidopsis thaliana.

The invention belongs to the field of application of biological extracts, and particularly relates to application of a rhizoma corydalis extract. The rhizoma corydalis extract is used for improving and / or treating myocardial infarction or depression or myocardial infarction with depression; the rhizoma corydalis extract contains alkaloid; the alkaloid comprises dehydrocorydaline and / or tetrahydropalmatine and / or berberine and / or dehydrocorydaline and / or tetrahydropalmatine and / or berberine, and at least comprises dehydrocorydaline; the corydalis tuber extract is used for preparing medicines and / or health foods for improving and / or treating myocardial infarction or depression or myocardial infarction with depression. The effectiveness of the rhizoma corydalis extract in the aspect of treating myocardial infarction with depression and the effective and safe dosage are verified through experiments, and corresponding bases and effective references are provided for guiding and developing application of the rhizoma corydalis extract and application of the rhizoma corydalis extract in the aspect of preparing corresponding medicines and health-care foods.

The present application relates to a kind of tetrahydropyrrolizino [2, 3-c] quinoline alkaloid compound and derivative with novel structure and preparation method and purposes thereof.The new compound bungeanoline G provided in the present application is extracted and separated from Papaveraceae Corydalis bungeana Turcz.The present application also proposes a method for artificially synthesizing bungeanoline G, and a series of derivatives of bungeanoline G are prepared.Biological activity experiments show that the compounds have acetylcholinesterase and butyrylcholinesterase inhibitory activity, and can be applied in the preparation of drugs for treating or improving various neurodegenerative diseases such as Alzheimer's disease, vascular dementia, myasthenia gravis and the like.

A composition of traditional Chinese medicine (TCM) comprising Corydalis Rhizoma, Paeoniae Radix, Magnoliae Officinalis Cortex, and Sennae Folium; a composition of extracts of the TCM composition; a pharmaceutical composition thereof; a method of using the same for treating constipation, including constipation-predominant symptoms (IBS-C); and a method of preparing the compositions. The various compositions disclosed herein are safe and effective in treating constipations.

The invention discloses a Tibetan medicine Banjiaojiaopian pellet preparation, which comprises raw materials and auxiliary materials, the raw materials comprise the following components: gentiana algida, agilawood, choerospondias axillaris, white sandalwood, myristica fragrans, tabasheer, alpine horseradish, costus root, fructus chebulae, fructus terminaliae billericae, emblic leafflower fruit, tinospora sinensis merr, corydalis impatiens, clove and liquorice, and the auxiliary materials comprise an adhesive and a filler microcrystalline cellulose; the technological parameters for preparing the pellet preparation by adopting centrifugal granulation are as follows: the liquid spraying speed is 50ml / min, the number of revolutions of a turntable is 30r / min, and the air inlet temperature is 75 DEG C. Compared with traditional pills, the pellet preparation provided by the invention has the advantages of high dissolution rate, obviously improved bioavailability, reliable drug effect, accurate taking dosage, stable quality, low cost, good treatment effect, fine production process, good reproducibility, good quality controllability, high industrial production efficiency and wide application range.

The present application relates to a kind of new isoquinoline alkaloid compound and its preparation method and purposes.The new compound (Corybungine A-L) provided by the present application is extracted and separated from Papaveraceae Corydalis bungeana Turcz., a kind of novel isoquinoline alkaloid with novel structure.Biological activity experiment shows that the alkaloid compound has dopamine D2 receptor antagonistic activity, and can be applied in the preparation of dopamine D2 receptor related pain, schizophrenia, Lesch-Nyhan syndrome and other nervous system diseases.

The invention discloses a rapid propagation and breed conservation method for Corydalis sutchuenensis, and relates to the technical field of plant biologication.According to the rapid propagation and breed conservation method for Corydalis sutchuenensis, Corydalis sutchuenensis seeds are used as explants, and the steps of graded disinfection of the explants, pretreatment, callus and bud induction culture, anti-pollution subculture, rooting culture and sterile transplanting are sequentially carried out, so that the Corydalis sutchuenensis is obtained. The method specifically comprises the following steps: S1, carrying out graded disinfection on explants: selecting full and undamaged corydalis pallidiflora seeds, washing the seeds with clear water for 10-15 minutes, then sequentially soaking the seeds with a 5% sodium hypochlorite solution for 2-3 minutes, 75% ethanol for 40-60 seconds and a 0.1% mercury bichloride solution for 10-12 minutes, washing the seeds with sterile water for 4-6 times after each step of disinfection, and finally absorbing surface moisture with sterile filter paper; according to the rapid propagation and breed conservation method for the corydalis tuber, efficient callus induction and bud proliferation are realized through optimized hormone proportion and culture conditions, and the requirements of large-scale and commercial seedling production and breed conservation can be met.

The application discloses a quality control method of radix stephaniae tetrandrae decoction pieces, adopts liquid chromatography-mass spectrometry to detect the efficacy components in the radix stephaniae tetrandrae decoction pieces, and the efficacy components include any one or several of tetrandrine, fangchinoline, cycilanol, stepholidine, oxystepholidine, stephaoxime, corynoxeine, miliradin and oxymatrine. The specific detection condition can detect multiple efficacy components in the radix stephaniae tetrandrae decoction pieces, which is helpful for comprehensively controlling the quality of the radix stephaniae tetrandrae decoction pieces, and finding the possibility of replacing wild radix stephaniae tetrandrae through efficacy evaluation. In addition, the thin layer chromatography is used to identify the radix stephaniae tetrandrae decoction pieces before detection, and the identification sensitivity can be improved by adjusting the thin layer chromatography condition.

The present invention belongs to the field of medical technology, and specifically relates to the use of corydaline or a pharmaceutically acceptable salt thereof in the preparation of a medicament for treating rheumatoid arthritis. Experimental results show that corydaline can effectively alleviate the level of paw swelling in CIA rats, reduce arthritis scores, and inhibit the progression of rheumatoid arthritis. Routine blood analysis shows that administration of corydaline reduces the number of immune cells in the blood and weakens the body's immune level. In addition, experimental results also show that corydaline can inhibit synovial hyperplasia and alleviate joint damage. Therefore, corydaline has the prospect of being developed into an oral medication for treating rheumatoid arthritis, providing more treatment options for the clinical treatment of rheumatoid arthritis, and has important social benefits and economic value.

The present invention belongs to the technical field of active compound extraction, and specifically relates to a total alkaloid extract of Corydalis dwarf with activity in preventing and treating hyperuricemia, as well as a preparation method and application thereof. The present invention comprises the following steps: extracting the medicinal material of Corydalis dwarf with an ethanol solution through cold soaking, filtering the extract and concentrating it under reduced pressure to obtain an extract concentrate; dissolving the extract concentrate in a dilute hydrochloric acid aqueous solution, extracting it with ethyl acetate while shaking and dropwise adding concentrated ammonia water, and then post-processing the extracted portion to obtain a total alkaloid extract of Corydalis dwarf. The present invention conducts in-depth and systematic research on the secondary metabolites of the total alkaloids of Corydalis dwarf and their in vitro xanthine oxidase inhibitory activity, in vivo uric acid-lowering activity and kidney-protective activity, providing detailed data support for the clinical application of the Tibetan medicine Corydalis dwarf. The preparation method provided by the present invention can effectively extract the effective active ingredients in Corydalis dwarf, and the extracted extract has xanthine oxidase inhibitory activity, in vivo uric acid-lowering activity and kidney-protective activity.

The application discloses a demethylase ODM gene for synthesizing benzyl isoquinoline alkaloids and application thereof, and the demethylase ODM gene is an ODM4 gene or an ODM5 gene, the CDS region nucleotide sequence of the ODM4 gene is shown as SEQ ID NO:1, and the CDS region nucleotide sequence of the ODM5 gene is shown as SEQ ID NO:2. In view of the fact that the research foundation for key genes for synthesizing corydaline in Corydalis tuber is weak, the ODM4 gene for catalyzing the demethylation of tetrahydropalmatine to specifically generate corydaline and tetrahydrojatrorrhizine and the ODM5 gene for specifically generating tetrahydrojatrorrhizine are cloned from the Corydalis tuber for the first time. The application provides an important theoretical basis for producing corydaline by using a synthetic biology method in the future, and has wide application prospect and great economic value.

The invention relates to a corydaline 9-O-methyltransferase PaOMT1, a coding gene of the corydaline 9-O-methyltransferase PaOMT1 and a function of the corydaline 9-O-methyltransferase PaOMT1, wherein the corydaline 9-O-methyltransferase PaOMT1 is used for catalyzing (S)-corydaline in Phellodendron amurense of rutaceae to form (S)-tetrahydrocotetrandrine. Belongs to the technical field of gene engineering. The invention discloses an open reading frame sequence of amur corktree PaOMT1 gene and an amino acid sequence coded by the amur corktree PaOMT1 gene. Candidate OMT genes are screened on the basis of amur corktree genome, transcriptome and metabolome data, and functions of the amur corktree OMT genes are verified in vitro by constructing a pET28a-OMTs prokaryotic expression vector and taking (S)-corydaline as a substrate. The result proves that the PaOMT1 has the function of catalyzing the (S)-golden corydalis alkali to form the (S)-tetrahydrocolumbamine, in addition, the PaOMT1 can further catalyze the (S)-tetrahydrocolumbamine to form the (S)-tetrahydropalmatine, and a foundation is laid for the biological research on synthesis of the protoberberine alkaloid with important medicinal value.