Method for inducing rubber tree corynespora cassiicola fungi to produce spores

Abstract

The invention relates to a method for inducing rubber tree corynespora cassiicola fungi to produce spores, and belongs to the technical field of bioscience. The method includes the steps of preparing inoculation rubber seedlings, culturing corynespora cassiicola fungi, inoculating corynespora cassiicola fungi and inducing corynespora cassiicola fungi to produce spores. The method has the advantages that corynespora cassiicola fungi are kept growing in original hosts, the growth and spore production environment of corynespora cassiicola fungi is similar to a wild environment, the form of produced spores is consistent with the form of spores formed under wild conditions, and it is avoided that spores of corynespora cassiicola fungi are varied by existing indoor artificial matrix culture; inoculation environment conditions can be manually regulated and controlled without being limited by seasons, operation is convenient, and manpower, material resources and time are saved.

Description

Technical field: [0001] The invention relates to a method for inducing sporulation of Corynespora multiprimum fungi of rubber trees, belonging to the technical field of life sciences. Background technique: [0002] Corynesporium defoliation of rubber trees is a devastating disease following the South American leaf blight. It can damage rubber seedlings, saplings and mature trees, and damage leaves of all ages, causing young leaves and shoots to wither, leaves A large number of repeated shedding, resulting in a reduction in latex production of up to 20% to 25%, and even the death of rubber trees. Domestic scholars have encountered in their research that the fungus is not easy to produce spores under indoor artificial culture conditions, which affects the morphological identification of pathogenic bacteria and related scientific research work. Some scholars have used indoor culture medium natural sporulation method, paper sporulation method and hyphae interruption method to i...

AI Technical Summary

Problems solved by technology

Domestic scholars have encountered in their research that the fungus is not easy to produce spores under indoor artificial culture conditions, which affects the morphological identification of pathogenic bacteria and related scientific research work

Some scholars have used indoor culture medium natural sporulation method, paper sporulation method and hyphae interruption method to induce sporulation of Corynesporacassiicola (Berk Curt) Wei strain of rubber tree, only a very small number of Corynespora fungi It can produce spores on the culture medium. Although it can produce spores by the method of paper disc and mycelia interruption, the shape of spores is quite different from that formed under natural conditions, so it is difficult to use for morphological identification.