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Method for detecting pancreatic tumor, antibody, and pancreatic tumor detection kit

A detection method and antibody technology, applied in chemical instruments and methods, measuring devices, anti-animal/human immunoglobulin, etc., can solve problems such as high practical barriers, difficult signal attribution, and unsuitability

Active Publication Date: 2016-06-01
TORAY IND INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, when a sample contains many different proteins, the signals obtained from each protein interfere with each other, making it difficult to assign signals
In addition, since there is also a problem with quantification, it is not suitable for diagnostic applications that require high-precision measurement
Therefore, there is a problem that the barrier to practical use is high

Method used

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  • Method for detecting pancreatic tumor, antibody, and pancreatic tumor detection kit
  • Method for detecting pancreatic tumor, antibody, and pancreatic tumor detection kit
  • Method for detecting pancreatic tumor, antibody, and pancreatic tumor detection kit

Examples

Experimental program
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Effect test

no. 1 approach

[0068] The first embodiment of the present invention is an anti-APOA2 antibody (comprising an anti-APOA2 protein terminal antibody and an anti-APOA2 protein non-terminal antibody) and fragments thereof.

[0069] 1-1. Anti-APOA2 antibody

[0070] In the present specification, the term "APOA2 protein" refers to APOA2 proteins of various species, but preferably human-derived APOA2 proteins (GenBank Accession No. NP_001634.1). Specifically, it includes the human-derived wild-type APOA2 protein variant shown in SEQ ID NO: 1, 2 or 3, and further includes natural mutants and fragments thereof.

[0071] In this specification, the aforementioned "variants" refer to different molecular forms of the APOA2 protein that can exist in plasma, serum or other body fluids of humans or animals. For example, APOA2 proteins that differ in the structure of the C-terminal region in APOA2 proteins or natural mutants thereof. Specifically, APOA2 protein variants, for example, the APOA2-ATQ protein s...

Embodiment 1

[0209] (Example 1) Production of a monoclonal antibody (anti-APOA2-ATQ terminal monoclonal antibody) that specifically recognizes the C-terminal region of the APOA2-ATQ protein

[0210] (a) Production of cells that produce antibodies that recognize the C-terminal region of APOA2-ATQ protein

[0211] A peptide composed of the amino acid sequence shown in SEQ ID NO: 29, which is the sequence of the C-terminal region of the APOA2-ATQ protein, is poorly soluble in water and has low antigenicity, so it was synthesized by adding 3 arginines to the N-terminal side. residue to impart hydrophilicity, and a cysteine ​​residue was added to its N-terminus. Next, maleimide-activated ovalbumin (Maleimide-Activated Ovalbumin, manufactured by Pias Co., Ltd.) was used to bind the cysteine ​​residue of the aforementioned peptide to the OVA protein. Using this as an immunogen, 100 µg of the immunogen was intraperitoneally administered to mice (BALB / c) at intervals of 2 weeks. From the first im...

Embodiment 2

[0217] (Example 2) Detection of APOA2-ATQ protein by ELISA method using anti-APOA2-ATQ terminal monoclonal antibody

[0218] APOA2-ATQ protein was detected by ELISA using the anti-APOA2-ATQ terminal monoclonal antibody 7F2 or 6G2 obtained in Example 1. The recombinant human-derived APOA2-ATQ protein, APOA2-AT protein or APOA2-A protein was adjusted to 1 μg / mL with PBS solution, and then 100 μL was added to the wells of the immunoplate Maxisorp, and immobilized overnight. On the next day, the aforementioned solution was discarded, 400 μL of blocking buffer A solution was added, and the mixture was left to stand at room temperature for 1 hour. After discarding the solution in the well, add 400 μL of PBS-T for washing, and add 100 μL of antibody 7F2 or 6G2, react at room temperature for 2 hours. After discarding the solution in the well, it was washed with PBS-T, and then 100 μL of polyclonal rabbit anti-mouse immunoglobulin / HRP diluted 5000 times with a diluent was added and r...

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Abstract

A method, system and storage device are generally directed to determining for each of a plurality of data points, a neighborhood of data points about each such data point. For each such neighborhood of data points, a projection set of polynomials is generated based on candidate polynomials. The projection set of polynomials evaluated on the neighborhood of data points is subtracted from the plurality of candidate polynomials evaluated on the neighborhood of data points to generate a subtraction matrix of evaluated resulting polynomials. The singular value decomposition of the subtraction matrix is then computed. The resulting polynomials are clustered into multiple clusters and then partitioned based on a threshold.

Description

technical field [0001] The present invention relates to a method for detecting pancreatic tumors, i.e., pancreatic cancer or benign pancreatic tumors, an anti-APOA2 antibody used in the method, and a kit for detecting pancreatic cancer or benign pancreatic tumors comprising the antibody. The detection method includes the use of APOA2 protein Specific binding antibody (anti-APOA2 antibody) to determine the amount of APOA2 protein variants in the subject sample. Background technique [0002] According to the statistics of 2012, the first place of the main cause of death in Japan is cancer. Carcinoma is characterized by the formation of a tumor mass caused by abnormal growth of cancer cells arising from normal tissues, the infiltration of cancer cells forming a tumor mass into adjacent tissues, and distant metastasis to various organs via blood vessels and lymphatic vessels . It is known that the concentration of various proteins in the patient's body fluids such as blood and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574C07K16/18C12N15/02C12P21/08
CPCC07K16/18C07K16/303G01N2333/775G01N33/57438G01N33/57488G01N33/6857G01N33/577C07K2317/34C07K2317/565G01N2333/47
Inventor 真田光彰小林道元高山爱子笹岛义志郑基晚山田哲司本田一文
Owner TORAY IND INC
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