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Domestic fungus liquid spawn culture method and medium thereof

A strain culture medium and liquid strain technology, which is applied in the field of edible fungus liquid strain culture and edible fungus liquid strain culture medium, and can solve the problems that the influence of micro-nano bubble water on the growth of edible fungi has not been reported.

Inactive Publication Date: 2016-06-08
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there has been no relevant report on the effect of micro-nano bubble water on the growth of edible fungi

Method used

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  • Domestic fungus liquid spawn culture method and medium thereof
  • Domestic fungus liquid spawn culture method and medium thereof
  • Domestic fungus liquid spawn culture method and medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Embodiment 1, DO value 10mg / L micronano bubble water carries out Pleurotus eryngii liquid strain culture

[0094] Provide a predetermined amount of micro-nano bubble water with a DO value of 10 mg / L, and a predetermined amount of tap water with a DO value of 6 mg / L. Provide two media raw materials, which respectively include: white sugar 20g / L, soybean meal powder 3g / L, MgSO 4 0.6g / L, K 2 HPO 4 0.6g / L, respectively use micro-nano bubble water and tap water to dissolve to a constant volume to form two strain culture media.

[0095] Inject the same amount of Pleurotus eryngii strains into two strain culture media respectively. After 7 days of shaking culture at a constant temperature at 25°C, take 150ml of the culture solution from the above two culture solutions and filter out the bacteria balls with filter paper, wash them several times, then put them in a drying oven, dry them at 105°C for 2 hours, and dry them. Dry, weigh after cooling to determine the biomass of ...

Embodiment 2

[0098] Embodiment 2, DO value 15mg / L micro-nano bubble water carries out Pleurotus eryngii liquid strain culture

[0099] Provide a predetermined amount of micro-nano bubble water with a DO value of 15 mg / L, and a predetermined amount of tap water with a DO value of 6 mg / L. Provide two media raw materials, which respectively include: white sugar 20g / L, soybean meal powder 3g / L, MgSO 4 0.6g / L, K 2 HPO 4 0.6g / L, respectively use micro-nano bubble water and tap water to dissolve to a constant volume to form two strain culture media.

[0100] Inject the same amount of Pleurotus eryngii strains into two strain culture media respectively. After 7 days of shaking culture at a constant temperature at 25°C, take 150ml of the culture solution from the above two culture solutions and filter out the bacteria balls with filter paper, wash them several times, then put them in a drying oven, dry them at 105°C for 2 hours, and dry them. Dry, weigh after cooling to determine the biomass of...

Embodiment 3

[0104] Embodiment 3, DO value 15mg / L micro-nano bubble water carries out the Pleurotus eryngii liquid strain cultivation of different time

[0105] Provide a predetermined amount of micro-nano bubble water with a DO value of 15 mg / L, and a predetermined amount of tap water with a DO value of 6 mg / L. Provide two media raw materials, which respectively include: white sugar 20g / L, soybean meal powder 3g / L, MgSO 4 0.6g / L, K 2 HPO 4 0.6g / L, respectively use micro-nano bubble water and tap water to dissolve to a constant volume to form two strain culture media.

[0106] Inject the same amount of Pleurotus eryngii strains into two strain culture media respectively. After constant temperature shaking culture at 25°C for 6 days and 7 days respectively, take 150ml of the culture liquid from the above two culture liquids and filter out the bacterial balls with filter paper, wash them several times, then put them into a drying oven, and dry them at 105°C for 2 hours , dried it, weighe...

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Abstract

The invention provides a domestic fungus liquid spawn culture method and a medium thereof; the method comprises the following steps: a, providing micro-nano bubble water and medium raw materials of preset quantity, adding the medium raw materials into the micro-nano bubble water so as to obtain the bacterial strain medium, wherein the medium raw materials comprise bacterial strain culture needed carbon source, nitrogen source and inorganic salt; b, planting domestic fungus bacterial strain into the bacterial strain medium for the culturing process. The micro-nano bubble water is employed to replace tap water in the configuration of liquid spawn medium, and the novel medium is used for culturing the domestic fungus bacterial strain; compared with a conventional liquid spawn culture method, the novel method can obviously improve liquid spawn yield.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an edible fungus liquid strain culture medium and a method for cultivating edible fungus liquid strains using the medium. Background technique [0002] Micro-nano gas-liquid interface technology is an emerging discipline newly developed in the past ten years. Through this technology, "micro-nano bubbles" can be generated in the liquid, thereby forming micro-nano bubble water. Micro-nano bubbles refer to tiny bubbles with diameters ranging from 50 microns to tens of nanometers when bubbles occur. [0003] Compared with ordinary water, micro-nano bubble water has a great degree of dispersion, and there are about tens of millions of nano-micro bubbles in every milliliter of micro-nano water. The oxygenation effect of micro-nano bubbles in water is quite high, and the dissolved oxygen (DO) in a wide range of water can be rapidly increased in only a few hours. In addition, d...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 王倩朱燕华李巧珍黄建春周峰陈辉
Owner SHANGHAI ACAD OF AGRI SCI
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