A kind of method of cultivating golden clematis with culture bag

A culture bag and clematis technology, applied in the directions of botanical equipment and methods, horticultural methods, applications, etc., can solve the problems of large space occupied by glass culture bottles, fragile glass culture bottles, limited number of culture bottles, etc. The effect of shortening time, improving induction rate, improving rooting rate and root quality

Inactive Publication Date: 2018-06-05
NANPING HUAKE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, glass culture bottles are fragile and difficult to carry, and because glass culture bottles take up a lot of space, the number of culture bottles to be processed at one time is limited when it is sterilized, which will reduce its production efficiency
More importantly, since the culture bottle and bottle cap are sealed with a screw, the bottle mouth and the bottle cap need to be rotated and burned before the inoculation operation. The sterilization operation is complicated, time-consuming, and easy to cause contamination due to improper operation. Bacteria, so that the entire bottle of cultured seedlings was discarded

Method used

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  • A kind of method of cultivating golden clematis with culture bag
  • A kind of method of cultivating golden clematis with culture bag
  • A kind of method of cultivating golden clematis with culture bag

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) A transparent sealed plastic bag made of polypropylene material was used as the culture bag. The height of the bag was 14 cm, the bottom length was 5 cm, and the bottom width was 4 cm; On the side wall of the bag body, there is a breathable filter device 6 cm away from the mouth of the bag; the breathable filter device includes a tubular body used to connect the inner cavity of the bag with the atmosphere, its height is 4 mm, and the diameter of the nozzle is 3.5 mm ; The outer end of the tubular body is provided with a disc-shaped body for fixing it on the bag, and its diameter is 12 mm; the disc-shaped body is provided with a diameter of 8 mm and a hole diameter of 0.18 μm at the communication port between the tubular body and the atmosphere. Bacteria-filtering breathable membrane; place the culture bag in the support frame, and then put it into the outer packaging bag for high-temperature sterilization (such as figure 1 , 2 , 3);

[0031] 2) In the medium of MS...

Embodiment 2

[0038] 1) A transparent sealed plastic bag made of polypropylene material was used as the culture bag. The height of the bag was 16 cm, the length of the bottom was 7 cm, and the width of the bottom was 5 cm; And on the side wall of the bag body, a breathable filter device is provided at a place 8 cm away from the mouth of the bag; the breathable filter device includes a tubular body used to communicate with the inner cavity of the bag and the atmosphere, its height is 5 mm, and the diameter of the nozzle is 3.8 mm. mm; the outer end of the tubular body is provided with a disc-shaped body for fixing it on the bag, and its diameter is 13 mm; the disc-shaped body is provided with a diameter of 9 mm and a hole diameter of 0.2 μm at the communication port between the tubular body and the atmosphere. The bacteria-filtering air-permeable membrane; the culture bag is placed in the support frame, and then put into the outer packaging bag for high-temperature sterilization;

[0039] 2)...

Embodiment 3

[0046] 1) A transparent sealed plastic bag made of polypropylene material was used as the culture bag. The height of the bag was 18 cm, the length of the bottom was 8 cm, and the width of the bottom was 7 cm; And on the side wall of the bag body, a breathable filter device is provided at a place 10 cm away from the mouth of the bag; the breathable filter device includes a tubular body used to communicate with the inner cavity of the bag and the atmosphere, its height is 6 mm, and the diameter of the nozzle is 4.0 mm. mm; the outer end of the tubular body is provided with a disc-shaped body for fixing it on the bag, and its diameter is 14 mm; the disc-shaped body is provided with a diameter of 10 mm and a hole diameter of 0.22 μm at the communication port between the tubular body and the atmosphere. Bacteria-filtering air-permeable membrane; place the culture bag in the support frame, and then put it into the outer packaging bag for high-temperature sterilization;

[0047] 2) I...

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Abstract

The invention discloses a method for cultivating anoectochilus formosanus by using a culture bag. A transparent sealing plastic bag is used for replacing a traditional glass culture bottle to serve as a culture container; and according to different growth phases of the anoectochilus formosanus, an induction culture medium, a proliferation culture medium, a strong seedling culture medium or a rooting culture medium is injected into the culture bag, so that anoectochilus formosanus explants are subjected to tissue culture and seedling culture. When the anoectochilus formosanus is subjected to the tissue culture by adopting the method, the pollution can be effectively controlled, and the contamination rate is smaller than 3%; an inoculation progress is simplified and damages to the seedlings are reduced; an inoculating speed is improved and anoectochilus formosanus tissue culture seedlings with strong disease resistance are obtained; and meanwhile, the operation of taking out seedlings from the culture bottle after bagged tissue culture also can be avoided, and integration of tissue culture, seedling growing and product transportation of the anoectochilus formosanus is realized, so that the production cost is greatly reduced, the yield is improved and the growth period is shortened.

Description

technical field [0001] The invention belongs to the technical field of planting and cultivation of traditional Chinese medicinal materials, and in particular relates to a method for cultivating Aureus aureus with a cultivation bag. Background technique [0002] Clematis ( Anoectochilus roxburghii (Wall.) Lindl.) is a rare medicinal plant of the genus Orchidaceae, also known as golden thread orchid, mosaic orchid, etc. It is mainly distributed in Fujian and other southern provinces of China. The whole herb is used as medicine. Its nature is flat and sweet, and it has the effects of clearing heat, cooling blood, dehumidification and detoxification. It has the reputation of "King of Medicine" and "Golden Grass" among the people, and it has many aspects such as ornamental value, making it It has broad development prospects. [0003] At present, the artificial cultivation of clematis mainly adopts glass culture bottles to carry out group cultivation of seedlings. However, glas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 饶宝蓉陈泳和黄小云吴勇军陈敏健
Owner NANPING HUAKE BIOTECH CO LTD
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