High-temperature fermentation method of Chinese herbal medicines
A technology of high-temperature fermentation and Chinese herbal medicine, which is applied in the forming or processing of animal feed, food processing, animal husbandry, etc., can solve the problems of non-starch polysaccharide enzyme loss, drug residue, and increase of drug-resistant strains, so as to improve growth-promoting performance, Effects of reduced energy consumption and cost, and increased degradation
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Embodiment 1
[0026] Embodiment 1: the thermostable Bacillus licheniformis ( Bacilluslicheniformis ) HL-3 was inserted into the LB liquid medium cooled after sterilization, and cultured at 55°C and 150r / min for 12h to prepare Bacillus licheniformis ( Bacilluslicheniformis ) HL-3 seed solution for use. Glycyrrhizae is crushed and passed through a 60-mesh sieve for later use. Weigh 100 kilograms of crushed licorice, add 5000 grams of bran, 380 grams of calcium chloride, 150 grams of ferrous chloride, 500 grams of ammonium sulfate, 600 grams of dipotassium hydrogen phosphate and 90 kilograms of water, and mix well to obtain The fermentation culture of; Add 8 kilograms of Bacillus licheniformis ( Bacilluslicheniformis ) HL-3 seed solution, stirred and mixed evenly; the inoculated fermentation medium was divided into shallow fermentation pans that had been sterilized with 75% alcohol, the thickness of the material in the shallow pan was 4 cm, and the surface of the material in the shallow pan ...
Embodiment 2
[0027] Embodiment 2: the thermostable Bacillus licheniformis ( Bacilluslicheniformis ) HL-3 was inserted into the LB liquid medium cooled after sterilization, and cultured at 55°C and 150r / min for 12h to prepare Bacillus licheniformis ( Bacilluslicheniformis ) HL-3 seed solution for use. Glycyrrhizae and Radix Astragali are crushed and passed through a 60-mesh sieve for later use. Weigh 100 kg of crushed Chinese herbal medicine (70 kg of licorice + 30 kg of astragalus), and add 6000 g of bran, 390 g of calcium chloride, 260 g of ferrous chloride, 580 g of ammonium sulfate, and 680 g of dipotassium hydrogen phosphate and 95 kilograms of water, the fermentation culture obtained after mixing; add 8 kilograms of Bacillus licheniformis ( Bacilluslicheniformis) HL-3 seed liquid, stir and mix evenly; the fermentation medium after inoculation is divided into fermentation trays that have been sterilized with 75% alcohol. Cover the sterilized wet gauze, and then place the tray on the...
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