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Composite biological agent microcapsule capable of improving sensory quality of tobacco

A technology of biological preparations and microcapsules, which is applied in the fields of tobacco, food science, and preparation of tobacco, etc., can solve the problems of alcoholization of tobacco leaves that have not been seen, and achieve the effect of shortening the aging time of tobacco leaves, shortening the aging cycle of tobacco leaves, and improving the effect

Active Publication Date: 2016-06-15
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there has been great progress in the research on maintaining microbial activity through microencapsulation technology, but there is no good report on the use of this technology in the alcoholization of tobacco leaves

Method used

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  • Composite biological agent microcapsule capable of improving sensory quality of tobacco
  • Composite biological agent microcapsule capable of improving sensory quality of tobacco
  • Composite biological agent microcapsule capable of improving sensory quality of tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The composite biological agent microcapsules provided in this example for improving the sensory quality of tobacco leaves are prepared according to the following steps.

[0047] (1) Preparation of core material:

[0048] Bacillus subtilis bacteria liquid is prepared as follows: take Bacillus subtilis strain, LB liquid medium, 30 ℃, 200rpm shaker culture amplification, use sterile culture medium as blank control, record the absorbance of the bacteria liquid at 600nm Value OD 600 , when OD 600 =6.0, as the application of bacterial agent, the count at this time shows that the number of bacteria in the bacterial liquid=7 billion / mL (specifically 7.02×10 9 cfu / mL);

[0049] The sphingomonas bacteria solution was prepared as follows: take the sphingomonas bacteria strain, inorganic salt liquid medium, 30 ℃, 200rpm shaker culture amplification, use the sterile culture solution as a blank control, record The absorbance value OD of the bacterial solution at 600nm 600 , when...

Embodiment 2

[0070] After blending HJY-0, HJY-1, and HJY-2 prepared in Example 1 with tobacco leaves in a certain proportion, the tobacco leaves were still placed in the tobacco warehouse for storage. During the storage of the tobacco leaves, samples were taken every two months. The treated tobacco leaves were evaluated. The quality changes of tobacco leaves under different treatments are introduced as follows.

[0071] The changes in the quality of tobacco leaves without adding bacterial agents (blank control) are shown in the table below:

[0072] .

[0073] When HJY-0 is used to treat tobacco leaves, add 1.0% of the mass of the tobacco leaves. The changes in the quality of the tobacco leaves after treatment are shown in the table below:

[0074] .

[0075] When HJY-1 is used to treat tobacco leaves, it is added at a rate of 2.0% of the mass of the tobacco leaves. The changes in the quality of the tobacco leaves after treatment are shown in the table below:

[0076] .

[0077]...

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Abstract

The invention belongs to the technical field of tobacco processing, in particular relates to a composite biological agent microcapsule capable of improving the sensory quality of tobacco. The composite biological agent microcapsule is prepared from a core material and a wall material which are mixed uniformly through spray drying, wherein the core material is a mixture of bacillus subtilis bacterial liquid, sphingol monad bacterial liquid and alkaline protease; the wall material is a mixed solution which is prepared by mixing corn syrup, Arabic gum and maltodextrin and has a 30 to 50 percent of mass concentration. According to the composite biological agent microcapsule disclosed by the invention, a microbial preparation and an enzymic preparation are coated through a microencapsulation technology, and a micro environment which is beneficial for survival of microorganisms is formed, so that the microbial preparation and biological enzyme can favorably take effect on the surface of the tobacco within a longer time, degradation of macromolecular substances in the tobacco is quickened, and the mellowing period of the tobacco is shortened; the effect of improving the quality of the tobacco is relatively obvious, the mellowing time of the tobacco can be obviously shortened, and for a tobacco production enterprise, the composite biological agent microcapsule has relative important application value.

Description

technical field [0001] The invention belongs to the technical field of tobacco processing, and in particular relates to a compound biological agent microcapsule for improving the sensory quality of tobacco leaves. Background technique [0002] Tobacco leaf aging is a key processing link to improve the sensory quality of tobacco leaves. After the tobacco leaves are harvested, threshed and re-roasted, it usually takes two to three years of aging cycle to achieve the best application quality. Improving the intrinsic quality of tobacco leaves and improving the industrial usability of tobacco through alcoholization has always been one of the hotspots in tobacco research. [0003] The essence of the alcoholization process of tobacco leaves is a synergistic process of various complex chemical degradation transformations, biological enzyme-catalyzed transformations, and microbial-promoted transformations. ) is degraded to form small molecular aroma compounds or aroma precursors, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20A24B3/12
CPCA24B3/12A24B15/20
Inventor 张展杨宗灿彭玉富周浩郝辉董艳娟高明奇刘向真聂聪马宇平
Owner CHINA TOBACCO HENAN IND
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