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Method for counting bacteria in liquid

A counting method and technology of bacteria, applied in biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve the problems of plant diseases, complicated operation, large errors, etc., and achieve low cost, simple detection process, and accurate identification Effect

Inactive Publication Date: 2016-06-15
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacteria are widely distributed in the environment, and the human body also has a considerable number of bacteria; it is estimated that the total number of bacterial cells in the human body and on the epidermis is about ten times the total number of human cells; bacteria are useful to the environment, humans and animals. Harm, some bacteria as pathogens can cause cholera, tuberculosis and other diseases, and also cause plant diseases; bacteria have a great impact on human activities, such as the production of yogurt, the production of antibiotics, the treatment of waste water, etc., are all related to bacteria Related, bacteria are also widely used in the field of biotechnology; in addition, some bacteria are also important detection indicators in the fields of environmental monitoring, food safety, and medical hygiene, such as the detection of Escherichia coli; traditional methods of detecting bacteria, such as coating Counting method, fermentation method and other methods are complex, time-consuming and have large errors

Method used

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  • Method for counting bacteria in liquid
  • Method for counting bacteria in liquid
  • Method for counting bacteria in liquid

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0057] like figure 2 As shown, when used, the dilution of the relative polluted water sample is 0.1, 0.01, 0.001, and three 1mL samples are taken for each dilution sample; each sample is equally divided into several droplets, and dropped on the surface of the superhydrophobic material 7 , to form a spherical droplet 2. Due to the refraction effect of the liquid, the droplet 2 can be similar to a convex lens. The laser generator 1 is used to inject a beam of laser light from one side of the droplet 2, and the light tends to expand and diverge from the The droplet 2 is ejected and projected onto the screen 4; the projection image is obtained by the camera device 5, and the obtained image is sent to the processing device 6, and the processing device 6 analyzes the obtained image, and the number of bacteria is obtained by using the MPN counting method; the superhydrophobic The material is a transparent material formed by loading tetramethylsilane and fluorine-containing silane on g...

specific Embodiment 2

[0058] When in use, dilute the relative contaminated water sample to 0.1, 0.01, 0.001, and take three 1mL samples for each dilution sample; turn the syringe 8 upside down, slowly push the piston to squeeze out the water sample, and the water sample flowing out of the needle forms a droplet 2 is suspended on the needle and keeps its shape; due to the refraction effect of the liquid, the droplet 2 can be similar to a convex lens, and the laser generator 1 is used to inject a beam of laser light from the side of the droplet 2, and the light is expanding. The divergent trend of the beam is ejected from the droplet 2 and projected onto the screen 4; the projection image is obtained by the camera device 5, and the obtained image is sent to the processing device 6, and the processing device 6 analyzes the obtained image, and obtains the bacteria by using the MPN counting method. quantity.

specific Embodiment 3

[0059] When in use, the dilution of the relative polluted water sample is 0.1, 0.01, 0.001, and three 1mL samples are taken for each dilution sample; the peristaltic pump 9 is used to draw the water sample through the catheter, and the water sample slowly flows out from the other end of the catheter to form droplets 2, and can keep its shape unchanged; the peristaltic pump 9 can control the water output speed and water output, which can make the droplet size basically the same; due to the refraction of the liquid, the droplet 2 can be similar to a convex lens, and the laser generator 1 can be used to A beam of laser light is injected from one side of the droplet 2, and the light beam is emitted from the droplet 2 and projected onto the screen 4 in a tendency of beam expansion and divergence; the projected image is acquired by the camera device 5, and the acquired image is sent to the processing device 6 , the processing device 6 analyzes the acquired image, and obtains the numb...

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Abstract

The invention discloses a method for counting bacteria in liquid.The method includes steps: taking a to-be-detected solution, selecting three continuous ten-time dilution gradients to obtain solutions of three dilutions; respectively taking three samples from the solution of each dilution to obtain A1, A2 and A3, and dripping drop by drop after forming of drops; emitting a laser beam to penetrate through the drops from one side of each drop to form projections, acquiring a projection image of each drop, subjecting the projection images to pattern recognition to judge whether bacteria exist or not, and counting the bacteria according to an MPN (most probable number) counting method.The method for counting the bacteria in liquid is short in time consumption, convenient and simple in operation, easy to implement and widely applicable to detection of the bacteria in liquid in the fields of environment monitoring, food safety, medical treatment and health care and the like.

Description

technical field [0001] The invention relates to a method for counting particles in liquid, in particular to a method for counting bacteria in liquid. Background technique [0002] Bacteria are the living organisms with the widest distribution and the largest number of individuals in nature, and are the main part of the material cycle in nature; they can be divided into cocci, bacilli, and spirochetes according to their basic forms. Bacteria are widely distributed in the environment, and the human body also has a considerable number of bacteria; it is estimated that the total number of bacterial cells in the human body and on the epidermis is about ten times the total number of human cells; bacteria are useful to the environment, humans and animals. Harm, some bacteria as pathogens can cause cholera, tuberculosis and other diseases, and also cause plant diseases; bacteria have a great impact on human activities, such as the production of yogurt, the production of antibiotics,...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 范美坤龚正君何雨希汤文浩孟凡昊侯玉卿
Owner SOUTHWEST JIAOTONG UNIV
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