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Mercury ion detection method

A detection method, mercury ion technology, applied in the field of nanotechnology, can solve the problems of strict use conditions, high cost, high cost, etc., and achieve the effect of high stability and low cost

Active Publication Date: 2016-06-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, enzymes are biocatalysts. Due to the inherent characteristics of enzymes, the conditions required for catalysis are relatively strict, and they require high costs, and their applications are also subject to some restrictions.
For example, protease is easy to denature and hydrolyze, the cost is high and the use conditions are strict

Method used

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Examples

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preparation example Construction

[0050] Specifically, the preparation method of the DNA-Ag / Pt nanocluster solution includes:

[0051] Add 30 μL, 2.0 μM single-stranded nucleic acid (ssDNA), 5 μL, 150 μM silver nitrate solution and 12 μL, 125 μM potassium tetrachloroplatinate solution into a 200 μL PCR tube in sequence, and add 4 μL after reaction at 4°C for 30 min in the dark. A 5.0 mM sodium borohydride solution was shaken at 37 °C for 3 h to obtain a 2.0 μM DNA-Ag / Pt nanocluster solution.

Embodiment 1

[0054] (1) Synthesize DNA-Ag / PtNCs according to the reported references, and the specific steps are as follows:

[0055] Add 30 μL, 2.0 μM single-stranded nucleic acid, 5 μL, 150 μM silver nitrate solution and 12 μL, 125 μM potassium tetrachloroplatinate solution to a 200 μL PCR tube in sequence, and then add 4 μL, 5.0 mM potassium tetrachloroplatinate solution at 4°C for 30 min in the dark. The sodium borohydride solution was shaken at 37 °C for 3 h to obtain a 2.0 μM DNA-Ag / Pt nanocluster solution.

[0056] (2) Take 8 μL of the final mixed solution obtained in the step of diluting 2 times with ultrapure water, and add 2 μL of Tween-20 with a concentration of 0.005%-0.05% and 30 μL of sodium citrate with a pH value of 3.0 to 4.5 respectively. Buffer solution, 20 μL hydrogen peroxide with a concentration of 0.5M to 2.0M, 40 μL TMB solution with a concentration of 1.0 mM to 4.0 mM, and 100 μL of a series of standard Hg at different concentrations 2+ The solution was uniformly ...

Embodiment 2

[0060] (1) Synthesize DNA-Ag / PtNCs according to the reported references, and the specific steps are as follows:

[0061] Add 30 μL, 2.0 μM single-stranded nucleic acid (refer to Example 1, but not limited to this), 5 μL, 150 μM silver nitrate solution and 12 μL, 125 μM potassium tetrachloroplatinate solution into a 200 μL PCR tube, 4 ℃ After reacting in the dark for 30 min, 4 μL of 5.0 mM sodium borohydride solution was added, and the solution was shaken at 37° C. for 3 h to obtain a 2.0 μM DNA-Ag / Pt nanocluster solution.

[0062] (2) Step (1) of diluting 4 times with ultrapure water, 8 μL of the finally obtained mixed solution was added, and 2 μL of Tween-20 with a concentration of 0.005%-0.05% and 30 μL of sodium citrate with a pH value of 3.0 to 4.5 were added in turn. Buffer solution, 20 μL hydrogen peroxide with a concentration of 0.5M to 2.0M, 40 μL TMB solution with a concentration of 1.0 mM to 4.0 mM, and 100 μL of a series of standard Hg at different concentrations 2...

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Abstract

The invention discloses a mercury ion detection method. The mercury ion detection method comprises the following steps: adding a solution to be detected, which possibly contains Hg<2+>, Tween-20, a sodium citrate buffering solution, hydrogen peroxide and a TBM solution, into a double-metal nano cluster solution, and mixing for reacting; and detecting the concentration of Hg<2+> in the solution to be detected by observing a light absorption value of a visible light wave band of a mixing reaction system. The mercury ions (Hg<2+>) are detected colorimetrically by simulating peroxidase based on DNA-Ag / Pt nano clusters; a linear range of detection is 10.0nM-200nM and the sensitivity can reach 3.0nM; and the mercury ion detection method has the advantages of simplicity, convenience and rapidness, low cost, high stability and the like, and can be applied to detection of Hg<2+> in samples of environments, foods and the like.

Description

technical field [0001] The invention relates to a mercury ion detection method, in particular to a method for preparing bimetallic nanoclusters by using single-stranded nucleic acid and applying it to mercury ion detection, belonging to the field of nanotechnology. Background technique [0002] Enzymes are a class of proteins or nucleic acids with catalytic functions that can catalyze chemical reactions in a suitable environment. However, enzymes are biological catalysts. Due to the inherent characteristics of enzymes, the conditions required for catalysis are relatively strict, and they require high costs, and their applications are also limited. For example, protease is easy to denature and hydrolyze, the cost is high and the usage conditions are strict. Therefore, it is particularly important to develop a mimetic enzyme with similar catalytic activity. [0003] Nano-mimetic enzymes are a class of synthetic catalysts with non-protein structure but similar catalytic prope...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 彭池方吴亮亮王丽英谢正军
Owner JIANGNAN UNIV
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