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Method for constructing HPLC characteristic chromatogram of Chinese patent medicine 'Yishen Bugu liquid'

A technology of invigorating kidney and tonifying bone and characteristic spectrum, applied in the field of HPLC characteristic spectrum construction, can solve the problem of difficult and precise quality control, and achieve the effect of avoiding singleness and one-sidedness, good absorption value and good peak shape.

Active Publication Date: 2016-06-15
TONGYAO PHARMA GROUP CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the quality standard of this product, there are only traits, inspection, and identification items, and there are no content determination items. It is difficult to accurately control the quality of products in production.

Method used

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  • Method for constructing HPLC characteristic chromatogram of Chinese patent medicine 'Yishen Bugu liquid'
  • Method for constructing HPLC characteristic chromatogram of Chinese patent medicine 'Yishen Bugu liquid'
  • Method for constructing HPLC characteristic chromatogram of Chinese patent medicine 'Yishen Bugu liquid'

Examples

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Effect test

Embodiment 1

[0027] A method for constructing the HPLC characteristic spectrum of Yishenbuguye, comprising the following steps:

[0028] (1) Preparation of the test solution: The preparation of the test solution: take 5ml of this product, steam it to nearly dryness, add 25ml of water to dissolve it, add 25ml of diethyl ether and shake to extract, discard the diethyl ether, shake the water with ethyl acetate Extract 3 times, 25ml each time, combine the ethyl acetate solution, evaporate to dryness, add methanol to dissolve the residue and dilute it to a 5ml volumetric flask, shake well, filter, and take the subsequent filtrate as the test solution.

[0029] (2) Preparation of the reference substance solution: take an appropriate amount of the reference substance of naringin, weigh it accurately, add methanol respectively to make a solution containing about 0.1mg per 1ml, and obtain the solution.

[0030] (3) Determination: chromatographic conditions: use octadecylsilane bonded silica gel as ...

Embodiment 2

[0034] A method for constructing the HPLC characteristic spectrum of Yishenbuguye, comprising the following steps:

[0035] (1) Preparation of the test solution: Preparation of the test solution: take 10ml of this product, steam it to nearly dryness, add 30ml of water to dissolve it, add 30ml of diethyl ether to shake and extract, discard the diethyl ether, shake the water with ethyl acetate Extract 3 times, 30ml each time, combine the ethyl acetate solution, evaporate to dryness, add methanol to dissolve the residue and dilute it to a 5ml measuring bottle, shake well, filter, and take the subsequent filtrate as the test solution.

[0036] (2) Preparation of the reference substance solution: take an appropriate amount of the reference substance of naringin, weigh it accurately, add methanol respectively to make a solution containing about 0.1mg per 1ml, and obtain the solution.

[0037] (3) Determination: chromatographic conditions: use octadecylsilane bonded silica gel as fil...

Embodiment 3

[0039] A method for constructing the HPLC characteristic spectrum of Yishenbuguye, comprising the following steps:

[0040] (1) Preparation of the test solution: The preparation of the test solution: take 15ml of this product, steam it to nearly dryness, add 35ml of water to dissolve it, add 35ml of diethyl ether and shake to extract, discard the diethyl ether, shake the water with ethyl acetate Extract 3 times, 35ml each time, combine the ethyl acetate solution, evaporate to dryness, add methanol to dissolve the residue and dilute it to a 5ml measuring bottle, shake well, filter, and take the subsequent filtrate as the test solution.

[0041] (2) Preparation of the reference substance solution: take an appropriate amount of the reference substance of naringin, weigh it accurately, add methanol respectively to make a solution containing about 0.1mg per 1ml, and obtain the solution.

[0042] (3) Determination: chromatographic conditions: use octadecylsilane bonded silica gel as...

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Abstract

The invention provides a method for constructing an HPLC characteristic chromatogram of a Chinese patent medicine 'Yishen Bugu liquid'. The method comprises the following steps: (1) preparation of a solution of a tested object; (2) preparation of a solution of a reference substance: a proper amount of the naringin reference substance is taken and weighed precisely, methanol is added for preparing a solution, wherein every 1ml of the solution contains about 0.1mg of the substance; (3) determination: 5-15[mu]l of the solution of the reference substance and 5-15[mu]l of the solution of the tested object are separately and precisely absorbed, the solutions are injected into a high performance liquid chromatograph, chromatogram from the first minute to the twentieth minute is recorded, the chromatogram data is introduced into a <similarity evaluation system for chromatographic fingerprint applied in traditional Chinese medicine> 2004A edition for analysis, and the specific chromatogram of the 'Yishen Bugu liquid' is obtained. The method has the active effects that the established characteristic chromatogram has high technical content, unicity and one-sidedness of quality control of the 'Yishen Bugu liquid' preparation are avoided, high absorption conditions of near ultraviolet impurity peaks are avoided, and the possibility of artificial treatment of product quality for reaching the standard is reduced. The method has the advantages of multiple chromatograph peaks, good peak shape, easy identification, high similarity, good specificity, good stability, and reliable accuracy.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a method for constructing an HPLC characteristic map of a Chinese patent medicine. Background technique [0002] The standard of Yishenbuguye (composed of twelve medicinal materials such as Drynaria rhizome, Polygonum multiflorum, Poria, Dipsacus, Radix Paeoniae Alba, Angelica, Codonopsis pilosula, Rehmannia glutinosa, Polygonatum, etc.) is included in the tenth volume of Ministry-issued Standard Traditional Chinese Medicine Prescriptions, Standard number: (WS 3 -B-2020-95), discloses the quality control method of Yishenbuguye. In the quality standard of this product, there are only traits, inspection, and identification items, and there is no content determination item, so it is difficult to be accurate in quality control in product production. Contents of the invention [0003] The purpose of the present invention is to provide a method for ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 徐云王婷婷白冰徐建赵源慧李君海
Owner TONGYAO PHARMA GROUP CORP
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