Multi-drug resistance tuberculosis diagnosing marker set and application thereof

Abstract

The invention discloses a multi-drug resistance tuberculosis diagnosing marker set and application thereof. Sequences of multi-drug resistance tuberculosis diagnosing markers correspond to SEQ:ID:NO:1 to SEQ:ID:NO:8. The multi-drug resistance tuberculosis diagnosing markers are originated from 8 antigens of tuberculosis mycobacteria and used for judging whether a tested the optimal working point of protein combinations for diagnosing multi-drug resistance tuberculosis, the specificity is 80.4%, and the sensibility is 80%, wherein the specificity and the sensibility are both higher than indexes in diagnosis of multi-drug resistance tuberculosis in the prior art. In addition, in the technology, a serological test is adopted, samples are easy to obtain, and the multi-drug resistance tuberculosis diagnosing marker set is suitable for diagnosis of various kinds of suspicious multi-drug resistance tuberculosis including multi-drug resistance tuberculosis and extrapulmonary tubereulosis.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a group of multidrug-resistant tuberculosis diagnostic markers and applications thereof. Background technique [0002] Mycobacterium tuberculosis (Mycobacterium Tuberculosis, Mtb) is the pathogenic bacterium that causes tuberculosis. It is most common to invade the lungs and cause tuberculosis. It can also invade other tissues outside the lungs to cause tuberculosis in multiple organs and parts of the body. Due to inappropriate treatment and other reasons, more and more Mycobacterium tuberculosis has become resistant to mainstream anti-tuberculosis drugs, especially the emergence and spread of multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis has become the cause of the global tuberculosis epidemic recovery. One of the main reasons is the current difficult problem in the diagnosis and treatment of tuberculosis in the world, which has brought sever...

AI Technical Summary

Problems solved by technology

[0003] At present, the diagnosis of multidrug-resistant / extensively drug-resistant tuberculosis mainly relies on bacteriological and molecular biological methods to detect whether there are mutations in drug-resistance-related genes: 1. Traditional culture methods: the operation is cumbersome and time-consuming, and tuberculosis branches are isolated from clinical specimens. Bacillus usually ≥ 6 weeks, which is not conducive to early detection and diagnosis of diseases; 2. Polymerase chain reaction-single-strand conformation polymorphism analysis (PCR-SSCP method): there are many influencing factors (DNA fragment length, Gel concentration, etc.), poor reproducibility, not widely used in clinical work; 3, RT-PCR method: high requirements for detection conditions, limiting its promotion; 4, oligonucleotide probe hybridization method: Yes A rapid method for detecting Mycobacterium tuberculosis, but there are certain false negative and false positive rates, and the reagents are relatively expensive, so they have not been clinically promoted; and high cooling efficiency, but its application is limited due to the high requirements of analysis software and the high cost of chip production; 6. GeneXpertMTB / RIF can detect tuberculosis smear-negative patients and rifampicin-resistant tuberculosis within 2 hours, To facilitate the rapid diagnosis of tuberculosis, but in economically underdeveloped countries and regions, the application is still limited

[0004] In conclusion, current technologies fall short of rapid, effective, inexpensive and convenient diagnosis of MDR-TB

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