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A method of producing an amino acid fertilizer based on solid fermentation and enzymolysis of rapeseed meal

A solid fermentation and amino acid technology, applied in the field of microbial fermentation, can solve the problems of losing the advantage of low glucosinolates, wasting rapeseed meal resources, and increasing the content of glucosinolates, so as to increase the content of free amino acids and small peptides, increase the activity of soil microorganisms, and increase protein content. The effect of high degree of hydrolysis

Inactive Publication Date: 2016-06-22
TIANJIN JUNHEXIN TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Rapeseed is processed under modern industrial conditions, and about 55% of rapeseed cake can be obtained. Rapeseed cake is a high-quality vegetable protein resource, containing 35% to 45% of protein, but biological and mechanical hybrids in planting, purchasing and processing Both will lead to a significant increase in the content of glucosinolates, losing the advantage of low glucosinolates obtained by rapeseed breeding, and the traditional thermal processing process will destroy the quality of protein feed. Studies have shown that the use of rapeseed cakes in crop fertilizers can increase soil microbial activity , especially suitable for the planting of leaf vegetables, which overcomes the problems of slow decomposition of traditional organic fertilizers in the soil and excessive accumulation of nitrogen in leaves during the mature stage, which leads to the reduction of vegetable quality
However, using the traditional composting method to produce rapeseed meal organic fertilizer, the fermentation cycle is long, the nitrogen loss is serious, and a lot of rapeseed meal resources are wasted.

Method used

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  • A method of producing an amino acid fertilizer based on solid fermentation and enzymolysis of rapeseed meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Rapeseed meal, bran, water and lime are mixed and tempered in a weight ratio of 45:20:25:0.1 to prepare a fermented material wet base. Insert Stenotrophomonas maltophilia seed liquid and Bacillus pumilus seed liquid into the fermented material wet base, so that the weight ratio of the three is 100:5:8, and the bacterial liquid of Stenotrophomonas maltophilia and Bacillus pumilus The weight ratio is 3:7, stirred evenly, placed at room temperature, the fermentation period is 96h, the initial pH value of fermentation is 6, turned and thrown 4 times, solid fermentation, enzymatic hydrolysis, and drying to obtain amino acid fertilizer.

Embodiment 2

[0019] Rapeseed meal, bran, water and lime are mixed and tempered in a weight ratio of 48:18:30:0.1 to prepare a fermented material wet base. Insert Stenotrophomonas maltophilia seed liquid and Bacillus pumilus seed liquid into the wet base of fermentation material, so that the weight ratio of the three is 100:6:4, and the bacterial liquid of Stenotrophomonas maltophilia and Bacillus pumilus The weight ratio is 5:3, stirred evenly, placed at room temperature, the fermentation cycle is 120h, the initial pH value of fermentation is 7, turned and thrown 5 times, solid fermentation, enzymatic hydrolysis, and drying to obtain amino acid fertilizer.

Embodiment 3

[0021] Rapeseed meal, bran, water and lime are mixed and tempered in a weight ratio of 50:25:35:0.1 to prepare a fermented material wet base. Insert Stenotrophomonas maltophilia seed liquid and Bacillus pumilus seed liquid into the fermented material wet base, so that the weight ratio of the three is 100:5:5, and the bacterial liquid of Stenotrophomonas maltophilia and Bacillus pumilus The weight ratio is 1:1, stirred evenly, placed at room temperature, the fermentation period is 144h, the initial pH value of fermentation is 9, turned and thrown 6 times, solid fermentation, enzymatic hydrolysis, and drying to obtain amino acid fertilizer.

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Abstract

The invention belongs to the field of microbial fermentation, and particularly relates to a method of producing an amino acid fertilizer based on solid fermentation and enzymolysis of rapeseed meal. The method is characterized by comprising mixing the rapeseed meal, bran, water and lime, regulating to prepare a fermentation material wet medium, inoculating a certain inoculation amount of stenotrophomonas maltophilia and bacillus pumilus into the wet medium, fully stirring, and performing static solid fermentation at room temperature. After the fermentation is finished, the degree of hydrolysis of proteins in the rapeseed meal is obviously increased and the relative loss of the total nitrogen is low. Through applying the amino acid fertilizer prepared by the method into a crop growth environment, soil microbial activity can be increased. The fertilizer is particularly suitable for the leaf vegetable planting industry. The problem that quality of vegetables is lowered due to slow decomposition of traditional organic fertilizers in soil and excessive accumulation of nitrogen in leaves in a mature period is overcome.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for producing amino acid fertilizer based on solid fermentation enzymolysis of rapeseed meal. Background technique [0002] Rapeseed is processed under modern industrial conditions, and about 55% of rapeseed cake can be obtained. Rapeseed cake is a high-quality vegetable protein resource, containing 35% to 45% of protein, but biological and mechanical hybrids in planting, purchasing and processing Both will lead to a significant increase in the content of glucosinolates, losing the advantage of low glucosinolates obtained by rapeseed breeding, and the traditional thermal processing process will destroy the quality of protein feed. Studies have shown that the use of rapeseed cakes in crop fertilizers can increase soil microbial activity , especially suitable for the planting of leaf vegetables, which overcomes the problems of slow decomposition ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/00C05F11/00
CPCY02W30/40
Inventor 郑艾斌
Owner TIANJIN JUNHEXIN TECH DEV
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