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A kind of adipose mesenchymal stem cell osteogenic induction composition and its osteogenic induction method

A technology of stem cells and compositions, applied in the field of adipose-derived mesenchymal stem cells osteogenic induction compositions, can solve problems such as weak induction differentiation rate, and achieve the effects of reducing risks and immunogenicity, and improving the ability of osteogenic induction and differentiation

Active Publication Date: 2019-05-07
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when mesenchymal stem cells are induced by cadherin-11 alone, the induction differentiation rate is weak

Method used

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  • A kind of adipose mesenchymal stem cell osteogenic induction composition and its osteogenic induction method
  • A kind of adipose mesenchymal stem cell osteogenic induction composition and its osteogenic induction method
  • A kind of adipose mesenchymal stem cell osteogenic induction composition and its osteogenic induction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Primary isolation of adipose stem cells

[0048]After the adipose tissue and swelling fluid are separated naturally, absorb and discard the lower layer of liquid, add an equal volume of normal saline to wash the adipose tissue twice, and aspirate and discard the lower solution; divide the adipose tissue into 50mL centrifuge tubes, 20mL in each tube, Add an equal volume of 0.5% type Ⅰ collagenase (final concentration is 0.25%), mix well, seal, transfer to a constant temperature air shaker, digest at 37°C, 200R for 50min; centrifuge at 1500rpm for 10min after digestion; discard For the centrifuged fat and fat oil, add 40mL normal saline to each tube to resuspend the cells, centrifuge at 1500rpm for 5min, discard the supernatant, resuspend the cells with ordinary medium (DMEM / F12+10%FBS), and inoculate them into a culture dish , when the confluence of the cells reaches 80-90%, subculture is carried out.

[0049] 2. Identification of surface markers of human adipose ste...

Embodiment 2

[0072] 1. Primary isolation of adipose stem cells

[0073] After the adipose tissue and swelling fluid are separated naturally, absorb and discard the lower layer of liquid, add an equal volume of normal saline to wash the adipose tissue twice, and aspirate and discard the lower solution; divide the adipose tissue into 50mL centrifuge tubes, 20mL in each tube, Add an equal volume of 0.5% type Ⅰ collagenase (final concentration is 0.25%), mix well, seal, transfer to a constant temperature air shaker, digest at 37°C, 200R for 50min; centrifuge at 1500rpm for 10min after digestion; discard For the centrifuged fat and fat oil, add 40mL normal saline to each tube to resuspend the cells, centrifuge at 1500rpm for 5min, discard the supernatant, resuspend the cells with ordinary medium (DMEM / F12+10%FBS), and inoculate them into a culture dish , when the confluence of the cells reaches 80-90%, subculture is carried out.

[0074] 2. Identification of surface markers of human adipose st...

Embodiment 3

[0094] 1. Primary isolation of adipose stem cells

[0095] After the adipose tissue and swelling fluid are separated naturally, absorb and discard the lower layer of liquid, add an equal volume of normal saline to wash the adipose tissue twice, and aspirate and discard the lower solution; divide the adipose tissue into 50mL centrifuge tubes, 20mL in each tube, Add an equal volume of 0.5% type Ⅰ collagenase (final concentration is 0.25%), mix well, seal, transfer to a constant temperature air shaker, digest at 37°C, 200R for 50min; centrifuge at 1500rpm for 10min after digestion; discard For the centrifuged fat and fat oil, add 40mL normal saline to each tube to resuspend the cells, centrifuge at 1500rpm for 5min, discard the supernatant, resuspend the cells with ordinary medium (DMEM / F12+10%FBS), and inoculate them into a culture dish , when the confluence of the cells reaches 80-90%, subculture is carried out.

[0096] 2. Identification of surface markers of human adipose st...

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Abstract

The invention relates to the technical field of cell culture, in particular to an adipose-derived mesenchymal stem cell osteogenic induction composition and an osteogenic induction method thereof. The adipose-derived mesenchymal stem cell osteogenic induction composition comprises epithelial cadherin-11 and platelet rich plasma and can significantly improve the osteogenic induction differentiation capacity of adipose-derived mesenchymal stem cells; by adopting the PRP to replace animal serum, the application risk and immunogenicity of the composition are reduced, and the composition can be applied in clinic.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an osteogenic induction composition of adipose-derived mesenchymal stem cells and an osteogenic induction method thereof. Background technique [0002] Bone defect refers to the destruction of the structural integrity of the bone, which is a common clinical disease. Trauma, infection, tumor, surgical debridement of osteomyelitis, and various congenital diseases are the main causes of bone defects. Smaller bone defects (usually below 8mm) may heal on their own for a healthy non-smoker; but too large a bone defect, or a bone defect on a smaller bone, is difficult to heal completely. This requires surgical intervention, the main methods include: bone grafting, artificial bone and tissue engineered bone. [0003] Bone tissue engineering refers to the cultivation and expansion of isolated autologous high-concentration osteoblasts, bone marrow stromal stem cells or chondrocytes ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0654C12N2500/80C12N2501/998C12N2506/1384
Inventor 葛啸虎陈海佳王一飞马岩岩
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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