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Application of Kartogenin (KGN) to preparation of drugs for improving osteogenic differentiation capability of bone marrow mesenchymal stem cells (BM-MSCs)

A technology of bone marrow mesenchymal and osteogenic differentiation, applied in the field of cell medicine, can solve the problem of no related reports on KGN osteogenic induction, and achieve the effect of delaying the process of osteoporosis, promoting osteogenic differentiation and promoting bone healing.

Inactive Publication Date: 2018-11-09
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Kartogenin (KGN) was discovered by Kristen Johnson et al. in 2012 and found that it can induce cartilage formation by regulating the CBFb-RUNX1 transcriptional program, but there is no relevant report on KGN in terms of osteogenesis induction

Method used

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  • Application of Kartogenin (KGN) to preparation of drugs for improving osteogenic differentiation capability of bone marrow mesenchymal stem cells (BM-MSCs)
  • Application of Kartogenin (KGN) to preparation of drugs for improving osteogenic differentiation capability of bone marrow mesenchymal stem cells (BM-MSCs)
  • Application of Kartogenin (KGN) to preparation of drugs for improving osteogenic differentiation capability of bone marrow mesenchymal stem cells (BM-MSCs)

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Effect test

Embodiment 1

[0033] (1) Resuscitated bone marrow mesenchymal stem cells, inoculated at 175cm 2 Add 20 mL of complete medium to the culture flask, place at 37°C, 5% CO 2 Incubate in the incubator and change the medium every three days. Cells in the logarithmic growth phase were inoculated in multiple 12-well culture plates, complete medium was added, and the medium was changed every three days. After the cell density was greater than 90%, they were divided into groups, and osteogenic induction medium was added. At the same time, complete medium (CTRL group), 0.005% DMSO, 10nM KGN, 100nM KGN, and 1000nM KGN were added, and the medium was changed every three days. Alizarin red staining and quantification of calcium nodules were performed 14 days later.

Embodiment 2

[0035] (1) Resuscitated bone marrow mesenchymal stem cells, inoculated at 175cm 2 Add 20 mL of complete medium to the culture flask, place at 37°C, 5% CO 2 Incubate in the incubator and change the medium every three days. Cells in the logarithmic growth phase were inoculated in multiple 12-well culture plates, complete medium was added, and the medium was changed every three days. After the cell density was greater than 90%, they were divided into groups, and osteogenic induction medium was added. At the same time, complete medium (CTRL group), 0.005% DMSO, 10nM KGN, 100nM KGN, and 1000nM KGN were added, and the medium was changed every three days. After 14 days, the expression of osteogenesis-related genes and proteins was detected.

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Abstract

The invention discloses the application of KGN to preparation of drugs for improving the osteogenic differentiation capability of BM-MSCs, belonging to the field of cell medicine. Experimental resultsshow that KGN can promote the osteogenic differentiation of BM-MSC and improve the gene expression levels of SIRT1, ALP, COL1A1, RUNX2 and BGLAP and the expression contents of corresponding proteins.Therefore, the application of invention proves that KGN can promote the osteogenic differentiation potential of stem cells. The application KGN to preparation of drugs for improving the osteogenic differentiation capability of BM-MSCs in the invention can provide a brand-new bone-healing-capability-promoting scheme for medical research, tissue engineering research and the like.

Description

technical field [0001] The invention belongs to the field of cell medicine, and relates to the application of Kartogenin (hereinafter referred to as KGN) in the preparation of drugs for improving the osteogenic differentiation ability of bone marrow mesenchymal stem cells. Background technique [0002] Bone marrow mesenchymal stem cells (BM-MSCs) are a type of adult stem cells that reside in the bone marrow together with hematopoietic stem cells. Adult tissue-derived BM-MSCs are highly attractive in biological tissue engineering due to their self-renewal, high proliferation, and mesoderm differentiation potential, which can repair damaged tissues and promote tissue functional restoration in clinical practice. Today's academic circles generally believe that osteoblasts originate from BM-MSCs. Improving the osteogenic differentiation ability of BM-MSC has guiding significance for bone biology research and even clinical treatment. [0003] SIRT1 gene is a multifunctional tran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/192A61P19/08A61P19/10
CPCA61K31/192A61P19/08A61P19/10
Inventor 王一帆何帆陈曦闫金库李茂陈广东张文朱彩虹刘滔杨惠林
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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