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Preparation method of 2,3-butanediol through microbial fermentation

A technology of microbial fermentation and butanediol, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex processing methods and difficult scale application, etc., to reduce the difficulty of extraction and achieve high-efficiency glucose metabolism. , Well tolerated effect

Active Publication Date: 2016-06-29
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these processes can eliminate the influence of glucose on the separation process of 2,3-butanediol in different ways, the processing methods are complicated, and organic solvents are added to varying degrees, making it difficult to apply on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Seed liquid culture: Klebsiella bacteria preserved in liquid ( Klebsiella lapneumoniae ) 2mL of strain preservation solution was added to 800mL of LB medium, and mixed in a 1L seed tank for seed liquid cultivation. The culture conditions were controlled as follows: the culture temperature was 37° C., the stirring speed was set to 200 rpm, the pH was controlled at 7.0, micro-aerobic was maintained during the culture process, and the culture was cultivated for 11 hours to the logarithmic phase.

[0028] Take the liquid preserved Lactobacillus casei ( Lactobacillus casei ) 2.5mL of strain preservation solution was added to 1000mL of MRS medium, and mixed in a 1L seed tank for seed liquid cultivation. The culture conditions were controlled as follows: the culture temperature was 35°C, the stirring speed was set to 200rpm, the pH was controlled at 6.0, micro-aerobic was maintained during the culture process, and the culture was carried out for 20 hours to the logarithm...

Embodiment 2

[0036] (1) Seed liquid culture: Klebsiella bacteria preserved in liquid ( Klebsiella lapneumoniae) Add 3mL of strain preservation solution to 900mL of LB medium, mix it in a 1L seed tank, and carry out seed liquid culture. The culture conditions were controlled as follows: the culture temperature was 37°C, the stirring speed was set to 300rpm, the pH was controlled at 6.5, micro-aerobic was maintained during the culture process, and the culture was carried out for 15 hours to the logarithmic phase.

[0037] Take the liquid preserved Lactobacillus casei ( Lactobacillus casei ) 6mL of strain preservation solution was added to 1800mL of MRS medium, and mixed in a 2.5L seed tank for seed liquid cultivation. The culture conditions were controlled as follows: the culture temperature was 35°C, the stirring speed was set at 100 rpm, the pH was controlled at 6.0, micro-aerobic was maintained during the culture process, and the culture was carried out for 24 hours to the logarithmic p...

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Abstract

The invention discloses a preparation method of 2,3-butanediol through microbial fermentation; the method includes the steps: (1) strain culture: culturing lactobacillus with an MRS culture medium, to obtain a lactobacillus seed liquid; and culturing klebsiella pneumoniae with an LB culture medium, to obtain a klebsiella pneumoniae seed liquid; (2) fermentation culture: inoculating a fermentation culture medium with the klebsiella pneumoniae seed liquid, carrying out micro-aerobic fermentation, batch-feeding a glucose solution in the fermentation process to control the concentration of glucose in the fermentation system to be 20 g / L-40 g / L; and (3) fermentation control: in a fermentation last stage, adjusting the pH of the fermentation liquid to 5-6, inoculating with the lactobacillus seed liquid, and carrying out aerobic fermentation under a low-pH condition. The method can effectively remove residual glucose in the fermentation liquid, and is in favor of implementation of next-step two-aqueous-phase extraction, rectification and other refining ways, and is suitable for mass production and application.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method for preparing 2,3-butanediol by microbial fermentation. Background technique [0002] 2,3-butanediol can be used as a potential platform compound to replace traditional platform compounds for large-scale synthesis of methyl ethyl ketone (excellent solvent) and 1,3-butadiene (widely used in synthetic rubber, polyester and Polyurethane and other fields). In addition, 2,3-butanediol can also be used to prepare inks, perfumes, fumigants, moisturizers, softeners, plasticizers, explosives and pharmaceutical chiral carriers, etc., 2,3-butanediol Oxidation products 3-hydroxybutanone and diacetyl can be used as edible spices; at the same time, because of their high calorific value, which is equivalent to ethanol and methanol, they can be used as fuel additives; 2,3-butanediol can be produced after esterification The precursor of polyurethane foam; 2,3-butanediol re...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/22C12R1/245
Inventor 张霖樊亚超廖莎姚新武师文静
Owner CHINA PETROLEUM & CHEM CORP
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