Surface plasmon resonance sensor chip and cell response detection system and method

Abstract

The invention discloses a surface plasma resonance sensing chip and a cell response detection system and method. The sensing chip comprises a perfusion chamber, a culture chamber and a prism; the perfusion chamber is provided with a culture fluid flow inlet and a culture fluid flow outlet; the culture chamber is located below the perfusion chamber, the perfusion chamber is isolated from the culture chamber through an isolating layer and is in fluid communication with the culture chamber through the isolating layer; the prism is located under the culture chamber and tightly connected with the culture chamber in a sealed mode, and the surface, connected with the culture chamber, of the prism is plated with a noble metal film. The isolating layer can be a porous thin film, and the perfusion chamber can be provided with a specific structure for a culture fluid to conveniently and uniformly flow past. The sensing chip can supplement nutrition consumed by cells and discharge cell metabolism waste in real time, therefore, long-time growth of the cells on the sensing chip is maintained, and long-time cell detection is achieved; the influence of shear force produced by flowing of the culture fluid on cell growth can be significantly reduced, and the in-vivo growth state of the cells can be really simulated. Therefore, the sensing chip is simple in structure, and batched production is convenient.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a surface plasmon resonance sensor chip that can be used for long-term cell response detection. Background technique [0002] The surface plasmon resonance (Surface Plasmon Resonance, SPR) technology uses the optical principle to detect the slight change of the surface substance. SPR is the resonance of free electron waves present on the metal surface with incident light waves. These oscillations are influenced by the refractive index of the material adjacent to the metal surface and are thus used to detect small changes in the surface's refractive index, which form the basis of various sensing mechanisms. One of the necessary conditions for the formation of the SPR phenomenon is the existence of the interface between the metal film and the dielectric. When the incident light is incident at a certain angle, the attenuated total reflection phenomenon will occur due to the exi...

AI Technical Summary

Problems solved by technology

Although the sensor chip based on flow culture can be used for long-term cell culture, because the cell culture medium renewal rate is usually too fast, it will generate a large shear force on the cells and affect the normal growth of the cells, so the test results cannot truly reflect state of cells in the body

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