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Method for detecting nuciferine in lotus leaf through liquid chromatography

A liquid chromatography and nuciferine technology, applied in the field of laboratory testing, can solve the problems of long time, complicated extraction and detection process, etc., and achieve the effect of less sample consumption, consistent accuracy and short extraction time

Inactive Publication Date: 2016-06-29
WUZHOU INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

[0004] The pharmacopoeia method takes several hours to test, and the extraction and detection process is complicated and time-consuming

Method used

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  • Method for detecting nuciferine in lotus leaf through liquid chromatography

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Embodiment 1

[0025] A method for detecting nuciferine in lotus leaves by liquid chromatography, comprising the following steps:

[0026] Step 1: After crushing lotus leaves, sieve;

[0027] Step 2: Grinding after mixing lotus leaf powder and nano silicon dioxide, the weight ratio of lotus leaf powder and nano silicon dioxide is 19:1;

[0028] Step 3: Mix the mixture obtained in step 2 with an extraction solvent, the extraction solvent is a mixture of 1 part by weight of acetic acid, 4 parts by weight of methanol and 5 parts by weight of ethanol, and the weight ratio of the mixture to the extraction solvent is 1:9;

[0029] Step 4: After heating the mixture in step 3 to: 52°C, put the container containing the mixture into an ultrasonic cleaner for extraction to obtain an extraction mixture; the extraction time is 33 minutes; the ultrasonic frequency is 250KHz.

[0030] Step 5: After filtering the extraction mixture obtained in step 4, take 1ml and dilute it to 50ml with the extractant of t...

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Abstract

The invention discloses a method for detecting nuciferine in lotus leaves by liquid chromatography, which comprises the following steps: step 1: crush lotus leaves and sieve them; step 2: mix lotus leaf powder with nano silicon dioxide Post-grinding, step 3: mixing the mixture obtained in step 2 with an extraction solvent, step 4: placing the container containing the mixture in an ultrasonic cleaner for extraction to obtain an extraction mixture; step 5: filtering the extraction mixture and diluting it with an extraction agent , the diluted liquid chromatographic analysis is easy to determine the content of nuciferine. The invention provides a method for detecting nuciferin in lotus leaves by liquid chromatography, the method uses ultrasonic extraction to extract nuciferin and liquid chromatography to detect nuciferin, the detection accuracy is high, and the extraction and analysis operations are less difficult.

Description

technical field [0001] The invention relates to the field of laboratory detection, in particular to a method for detecting nuciferine in lotus leaves by liquid chromatography. Background technique [0002] The 2015 Pharmacopoeia recorded the extraction method of nuciferine in lotus leaves: take about 0.5g of the coarse powder of this product, accurately weigh it, put it in a stoppered conical flask, add 50ml of methanol accurately, weigh it, heat and reflux for 2.5 hours , let it cool, weigh again, make up for the lost weight with methanol, shake well, filter, accurately measure 5ml of the filtrate, put it in a 10ml measuring bottle, add water to the mark, shake well, and you get it. [0003] The detection conditions of liquid chromatography are as follows: octadecylsilane bonded silica gel is used as filler; acetonitrile-triethylamine glacial acetic acid (27:70.6:1.6:0.78) is used as mobile phase; detection wavelength is 270nm. The number of theoretical plates should not b...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陈学松王丽丽韦涛梁美艳黄新惠
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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