Method for extracting pine-seed oil

A technology of pine nut oil and pine nuts, which is applied in the field of edible vegetable oil extraction, can solve the problems of little research on the extraction of pine nut oil, and achieve the effects of low cost, simple equipment and broad application prospects

Inactive Publication Date: 2016-07-06
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are relatively few studies on the extraction of pine nut oil, and there are certain deficiencies in the existing extraction technology, and the method of extracting pine nut oil by ultrasonic-assisted aqueous enzymatic method has not been reported yet.

Method used

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  • Method for extracting pine-seed oil
  • Method for extracting pine-seed oil
  • Method for extracting pine-seed oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Clean the pine nuts, remove the shells and dry the pine nut kernels at a constant temperature until the quality is constant, crush them through a 60-mesh sieve, weigh 50g of pine nut kernel powder and place it in a reaction vessel. Pretreatment was carried out under the conditions of ℃ and 20 minutes; after the pretreatment, 2.5% cellulase was added, and the enzymatic hydrolysis and extraction was carried out for 3.0 hours under the conditions of temperature 45 ℃ and pH 4.0. After enzymatic hydrolysis, inactivate the enzyme in a boiling water bath for 10 minutes, then separate by centrifugation (centrifugal speed 3000r / min, 20min), take the free oil in the upper layer, and calculate the oil extraction rate, the extraction rate is 55.22%. (Note: The fat content of pine nuts is 58.12%, the same below).

Embodiment 2

[0023] Clean the pine nuts, remove the shells and dry the pine nut kernels at a constant temperature until the quality is constant, crush them through an 80-mesh sieve, weigh 50g of pine nut kernel powder and place it in a reaction vessel. Pretreatment was carried out under the conditions of 15min at ℃ and 15min; after the pretreatment, 2.0% cellulase was added, and enzymolysis and extraction were carried out at 45℃ and pH4.5 for 2.5h. After enzymatic hydrolysis, inactivate the enzyme in a boiling water bath for 10 minutes, and separate by centrifugation (centrifugal speed 3000r / min, 20min), take the free oil in the upper layer, and calculate the oil extraction rate, the extraction rate is 54.09%.

Embodiment 3

[0025] Clean the pine nuts, remove the shells and dry the pine nut kernels at a constant temperature until the quality is constant, crush them through an 80-mesh sieve, weigh 50g of pine nut kernel powder and place it in a reaction vessel. Pretreatment was carried out under the conditions of ℃ and 10 min; after the pretreatment, 2.0% cellulase was added, and the enzymatic hydrolysis and extraction was performed for 2.5 h at a temperature of 40 °C and a pH of 4.5. After enzymatic hydrolysis, inactivate the enzyme in a boiling water bath for 10 minutes, and separate by centrifugation (centrifugal speed 3500r / min, 20 minutes), take the free oil in the upper layer, and calculate the oil extraction rate, the extraction rate is 52.34%.

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Abstract

The invention relates to a method for extracting pine-seed oil through an ultrasonic-assisted aqueous enzymatic method, and belongs to the technical field of edible oil extraction. In the method, pine-seed powder is used as a raw material and pretreated with ultrasonic waves before enzymolysis to promote fast release of oil. The method includes the concrete steps that certain volume of distilled water is added into the pine-seed powder, and the mixture is placed into an ultrasonic reactor to be pretreated; after pretreatment is completed, a certain amount of cellulose is added into a water bath to extract the pine-seed oil in an enzymolysis mode; after enzymolysis is completed, an oil layer is centrifuged and collected to obtain the bright and transparent pine-seed oil. The method has the advantages of being simple in technology, high in extraction ratio, low in cost and free of organic solvent residual and is an environment-friendly extraction method, and the product quality meets the hygienic standard of national standard edible vegetable oil.

Description

technical field [0001] The invention relates to a method for extracting pine nut oil by an ultrasonic-assisted aqueous enzymatic method, belonging to the technical field of edible vegetable oil extraction. Background technique [0002] Pine nuts, also known as pine nuts and pine nuts, are the seeds of Pinus tabulaeformis, edible kernels, and are a special forest nut food in my country. It is widely distributed in western my country. "Compendium of Materia Medica" records: "Pine nuts are sweet in taste, warm in nature, and non-toxic. Indications: dizziness, rheumatism, moistening the five internal organs, alleviating hunger, expelling wind and cold, nourishing the body, moisturizing the skin, long-term use, lightening the body and not getting old, and moisturizing Lung function, cure dryness and cough". Because pine nuts are rich in protein, lipids, sugars, vitamins, minerals and physiologically active substances, they are a traditional food and medicine food with a history...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/10A23D9/02
CPCA23D9/02C11B1/025C11B1/04C11B1/10C11B1/108
Inventor 不公告发明人
Owner SICHUAN AGRI UNIV
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