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Lactobacillus plantarum CZ401 strain capable of producing exopolysaccharides and beta-galactosidase and application of strain

A technology of Lactobacillus plantarum and galactosidase, which is applied in the direction of glycosylase, enzyme, application, etc., can solve the problems of destroying and restoring the balance of the intestinal flora in the human body, and the survival rate is less than 0.1%.

Inactive Publication Date: 2016-07-06
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Streptococcus thermophilus and Lactobacillus bulgaricus, which make up the common yogurt starter, are mostly destroyed when they pass through the human digestive tract due to their intolerance to gastric acid and bile, and the survival rate is less than 0.1%, thus restoring the balance of the intestinal flora in the human body. level is very limited

Method used

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  • Lactobacillus plantarum CZ401 strain capable of producing exopolysaccharides and beta-galactosidase and application of strain
  • Lactobacillus plantarum CZ401 strain capable of producing exopolysaccharides and beta-galactosidase and application of strain
  • Lactobacillus plantarum CZ401 strain capable of producing exopolysaccharides and beta-galactosidase and application of strain

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Embodiment Construction

[0010] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0011] Isolation and identification of strains

[0012] In this example, intestinal microorganisms were isolated from cheese made by herdsmen in the prairie of Inner Mongolia and named CZ401. The morphological, physiological and biochemical characteristics of this strain are: the colony was light yellow when observed on the MRS solid medium, indicating smooth, It is round and raised in the center; the strain observed under the microscope is Gram-positive, rod-shaped, without flagella, and without spores.

[0013] Inoculate the CZ401 strain into 5ml of MRS culture solution (casein peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; glucose 5.0g; sodium acetate 5.0g; diamine citrate 2.0g; Tween 801.0g; hydrogen phosphate Dipotassium 2.0g; Magnesium sulfate heptahydrate 0.2g; Mangane...

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Abstract

The invention provides a lactobacillus plantarum (L.plantarum) CZ401 strain capable of producing exopolysaccharides and beta-galactosidase.The strain is preserved in China Center for Type Culture Collection on March 7, 2016, the address of the China Center for Type Culture Collection is Wuhan University, Wuchang District, Wuhan City of Hubei Province, the preservation number is CCTCC NO:M2016092, and the 16S rDNA sequence of the strain is shown as SEQ ID NO.1.When bacterial colonies are observed on an MRS solid medium, the bacterial colonies are light yellow, smooth in surface and circular, and the centers of the bacterial colonies protrude; it is shown through microscopy observation that the strain is positive in gram staining, rodlike, and free of flagella and spores.The CZ401 strain has higher capacity of producing exopolysaccharides and higher beta-galactosidase activity, can ferment cow's milk to produce lactic acid drinks and has wide application value.

Description

technical field [0001] The invention relates to a new strain of lactobacillus, in particular to a strain of lactobacillus CZ401 which efficiently produces exopolysaccharide and beta-galactosidase and its application. Background technique [0002] Lactic acid bacteria are recognized as a food-safe microorganism. At present, Streptococcus thermophilus and Lactobacillus bulgaricus, which make up the common yogurt starter, are mostly destroyed when they pass through the human digestive tract due to their intolerance to gastric acid and bile, and the survival rate is less than 0.1%, thus restoring the balance of the intestinal flora in the human body. levels are very limited. Lactobacillus plantarum can effectively tolerate enzymes in the digestive tract, gastric juice with a pH value, and bile acids. After entering the intestinal tract, it can regulate and improve the gastrointestinal tract and restore the balance of intestinal flora. In addition, Lactobacillus plantarum has t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12N9/38A23C9/123C12R1/25
CPCA23C9/1234C12N9/2471C12P19/04C12Y302/01023C12N1/205C12R2001/25
Inventor 程国军马成杰殷杰田梦洋张欢欢何冬兰李晓华彭杨
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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