Lactobacillus plantarum CZ401 strain capable of producing exopolysaccharides and beta-galactosidase and application of strain
A technology of Lactobacillus plantarum and galactosidase, which is applied in the direction of glycosylase, enzyme, application, etc., can solve the problems of destroying and restoring the balance of the intestinal flora in the human body, and the survival rate is less than 0.1%.
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[0010] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.
[0011] Isolation and identification of strains
[0012] In this example, intestinal microorganisms were isolated from cheese made by herdsmen in the prairie of Inner Mongolia and named CZ401. The morphological, physiological and biochemical characteristics of this strain are: the colony was light yellow when observed on the MRS solid medium, indicating smooth, It is round and raised in the center; the strain observed under the microscope is Gram-positive, rod-shaped, without flagella, and without spores.
[0013] Inoculate the CZ401 strain into 5ml of MRS culture solution (casein peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; glucose 5.0g; sodium acetate 5.0g; diamine citrate 2.0g; Tween 801.0g; hydrogen phosphate Dipotassium 2.0g; Magnesium sulfate heptahydrate 0.2g; Mangane...
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