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New application of clove leaf extract in the preparation of drugs against drug-resistant bacterial infection

A technology of leaf extracts and infectious diseases, which is applied in the field of new pharmacological applications of clove leaf extracts, and can solve the problems that related activities have not been reported yet

Active Publication Date: 2020-03-17
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies on the inhibition of drug-resistant bacteria by clove leaves, especially their related activities after oral administration, have not been reported yet.

Method used

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  • New application of clove leaf extract in the preparation of drugs against drug-resistant bacterial infection
  • New application of clove leaf extract in the preparation of drugs against drug-resistant bacterial infection
  • New application of clove leaf extract in the preparation of drugs against drug-resistant bacterial infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Grind 100g of clove leaves to 10-20 mesh, add 8 times the volume percentage of ethanol with a concentration of 70%, soak for 30 minutes, heat and reflux for 3 times, each time for 1.5h, filter, combine the extracts, and concentrate to a concentration of 1.06g / ml (50°C), add 2 times the volume of distilled water, let it stand for 30 minutes, centrifuge for 30 minutes (4000 rpm), take the supernatant and concentrate it to a relative density of 1.05g / ml to obtain the column solution, and the column solution is 4BV The flow rate per hour passes through the D101 macroporous adsorption resin chromatography column, the ratio of the dry weight of the upper column solution to the macroporous adsorption resin is 1:10, the diameter-height ratio of the macroporous adsorption resin column is 1:5, and then 15% of 5BV is used ethanol, discard the eluate; then elute 5BV with 60% ethanol, the elution rate is 4BV / h, collect the eluate, recover ethanol under reduced pressure, concentrate ...

Embodiment 2

[0026] Clove leaves 100g were pulverized into coarse powder, added 15 times volume percentage concentration of 90% ethanol, soaked for 60 minutes, heated and refluxed for 2 times, each time for 1h, filtered, combined extracts, concentrated to a concentration of 1.10g / ml ( 50°C), add 10 times the volume of distilled water, place it for 60 minutes, centrifuge for 45 minutes (3000 rpm), take the supernatant and concentrate it to a relative density of 1.10g / ml to obtain the column solution, and the column solution is 3BV / h The flow rate passes through the DM130 macroporous adsorption resin chromatography column. The ratio of the dry weight of the upper column solution to the macroporous adsorption resin is 1:12, and the diameter-to-height ratio of the macroporous adsorption resin column is 1:7. Remove and discard the eluent; then elute 6BV with 80% ethanol at an elution rate of 5BV / h, collect the eluate, recover ethanol under reduced pressure, concentrate to a relative density of 1...

Embodiment 3

[0028] Take 100g of clove leaves, add water and decoct twice, the first time for 1.5 hours, the second time for 1 hour, combine the decoction, filter, and concentrate the filtrate under reduced pressure to a clear paste with a relative density of 1.25 (80°C), add clove leaves 10g of fine powder, mix well, dry at 60-80°C, crush into fine powder, granulate with 75-85% ethanol, dry at low temperature, add appropriate amount of excipients, mix well, press into tablets to get Yanlixiao (i.e. clove blade).

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PUM

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Abstract

The invention relates to new application of natural drugs, in particular to new application of folium syringae extract in preparing drugs for resisting drug-resistant bacterium infection diseases.The folium syringae extract is prepared from, by weight, 19.91-30.39% of syringopicroside and 32.60-43.18% of total flavone and total phenolic acid of folium syringae, wherein the total weight percent of syringopicroside and total flavone and total phenolic acid of folium syringae is 52.51-72.99%.The extract has a good treatment effect on drug-resistant bacterium infections and has new application in being developed into drugs for resisting drug-resistant bacteria.

Description

technical field [0001] The invention relates to a new pharmacological application of the clove leaf extract, in particular to the use of the clove leaf extract in preparing drugs for inhibiting drug-resistant bacteria. The present invention also relates to clove leaf extract and its main components used as anti-drug-resistant bacterial infection drugs, pharmaceutical compositions containing them, anti-drug-resistant bacterial activities in vivo and in vitro, and usage methods. Background technique [0002] Bacterial infectious diseases are common and frequently-occurring diseases that endanger human health. Since the invention of penicillin, various antibiotics have played an important role in bacterial infectious diseases. After being put into use for only a few decades, they have become the most effective weapons against bacterial infections. , but with the frequent use of antibiotics, the limitations of their use have become increasingly prominent. In the past, a patient ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K31/12A61K31/185A61K36/61A61P1/00A61P1/12A61K127/00
CPCA61K31/7048A61K36/61A61K2236/333A61K2236/39A61K2236/55Y02A50/30
Inventor 殷军韩娜周正圆刘志惠
Owner SHENYANG PHARMA UNIVERSITY