Potato culture medium suitable for ustilaginoidea virens spore production and application thereof

A potato culture medium and rice smut fungus technology are applied in the fields of phytopathology technology and microbiology technology, and can solve the problems of multiple hyphae fragments, inability to provide spores stably, decrease in the number of spores, and the like, and achieve the effect of simple and convenient operation.

Inactive Publication Date: 2016-07-20
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are also some obvious deficiencies in the technology of preparing spores with this medium. After the sporulation peak, a large number of non-sporulation aerial hyphae grow quickly on the plate colony, resulting in a sharp de

Method used

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  • Potato culture medium suitable for ustilaginoidea virens spore production and application thereof
  • Potato culture medium suitable for ustilaginoidea virens spore production and application thereof

Examples

Experimental program
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Embodiment 1

[0032] Adopt a kind of potato culture medium suitable for rice smut fungus sporulation of the present invention and its application method, cultivate and prepare the thin-walled conidia of the rice smut fungus bacterial strain Uv-108, implement operation according to the following steps:

[0033] 1. Culture Medium Preparation

[0034] The proportioning ratio is: 100 g of potatoes, 20 g of agar, and 1000 ml of water to prepare a potato culture medium, which is sterilized by conventional high temperature for later use.

[0035] 2. Invert the culture plate

[0036] Melt the potato medium prepared in step 1 in a conventional manner and pour it into a 9 cm Petri dish to make a culture plate.

[0037] 3. Implantation of Aspergillus oryzae

[0038] Take out the aspergillus oryzae bacterial strain Uv-108 spore liquid (concentration is 10 6 individuals / ml), under aseptic conditions, 50 μl of the spore liquid was implanted into the culture plate prepared in step 2, and the spore liqu...

Embodiment 2

[0044] Adopt a kind of potato culture medium suitable for rice smut fungus sporulation of the present invention and its application method, cultivate and prepare the parenchyma conidia of rice smut fungus bacterial strain Uv-110, operate and implement according to the steps 1 to 5 of embodiment 1, only the steps 3 The strain implanted was Uv-110. After implementing the step 5 operation, wash a dish of flat plate colony with sterile water, can obtain 1.0×10 6 54ml of new spore liquid per spore / ml.

Embodiment 3

[0046] Adopt a kind of potato culture medium suitable for rice smut fungus sporulation of the present invention and its application method, cultivate and prepare the parenchyma conidia of rice smut fungus bacterial strain Uv-111, operate and implement according to the steps 1 to 5 of embodiment 1, only the steps 3 The strain implanted was Uv-111. After implementing the step 5 operation, wash a dish of flat plate colony with sterile water, can obtain 1.0×10 6 70ml of new spore liquid per spore / ml.

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Abstract

The invention discloses a potato culture medium suitable for ustilaginoidea virens spore production and an application thereof. Potatoes are prepared into a plate culture medium as a spore production culture medium so as to culture and prepare ustilaginoidea virens thin-wall conidiospore. Preparation for the culture medium and an application method for the culture medium comprise the following steps: 1) preparing the culture medium; 2) inverting a culture flat plate; 3) implanting ustilaginoidea virens; 4) carrying out constant-temperature culture; and 5) collecting a new generation of spores. The potato culture medium has the characteristics and the advantages that: the culture medium is simple in composition; the operation is simple, easy and convenient, and shaking culture equipment is not needed; time of obtaining a great number of spores is short; a high spore production state lasts for a long time; and a spore liquid is relatively pure, is less in mixed mycelia, and is generally suitable for being used in a lot of research work without purifying treatment.

Description

technical field [0001] The present invention relates to phytopathological techniques and microbiological techniques. Specifically, the invention relates to a potato culture medium suitable for spore production of rice false smut and an application method thereof. Background technique [0002] In most fungal disease systems, the propagules of pathogenic bacteria not only have the function of multiplying and expanding their own population, but more importantly, they have the function of interacting with and identifying the host, and initiating the function of infecting the host. Therefore, in many important plant disease research In the process, the propagules of pathogenic bacteria need to be used as test materials. Obviously, an efficient and convenient method for culturing and preparing propagule materials will be very conducive to the smooth development of research work. Rice false smut (caused by Ustilaginoideavirens (Cooke) Takahashi infection) has become a major diseas...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/645
CPCC12N3/00
Inventor 张君成王忠文曾东强蒲相君杨平
Owner GUANGXI UNIV
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