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A rapid test method for aflatoxin b1 based on a smart phone detection system

A technology of aflatoxin and smart phones, which is applied in the preparation of test samples, measuring devices, and material analysis through optical means, which can solve the problems of not being able to directly display the concentration of the detected substance, shorten the detection time, reduce the The effect of detection cost and low cost

Active Publication Date: 2018-08-24
TAIYUAN UNIV OF TECH
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Problems solved by technology

And Wong et al. (J. X. Wong, F. S. Liu and H-Z Yu, Mobile app-based quantitative scanometric analysis, Anal.Chem., 2014, 86, 11966-11971) have studied the quantitative detection by analyzing the optical gray scale ratio through the mobile phone application The purpose is to verify its performance by the reaction of biotin and streptavidin. Due to the limitation of the number of channels, it has certain limitations in multi-throughput detection and it only gives the optical gray scale ratio and cannot directly display the concentration value of the detected substance.

Method used

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  • A rapid test method for aflatoxin b1 based on a smart phone detection system
  • A rapid test method for aflatoxin b1 based on a smart phone detection system
  • A rapid test method for aflatoxin b1 based on a smart phone detection system

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Embodiment Construction

[0038] The specific embodiment of the present invention will be further described in detail below by taking cereals as an example.

[0039] as attached figure 1 to attach Figure 5 As shown, the specific steps for implementing the aflatoxin B1 rapid detection method based on the smart phone detection system provided above in the present invention are as follows.

[0040] (1) Preparation of aflatoxin B1 microchannel: Cut the transparent plastic sheet into a square of 5.5cm×6cm as the transparent base layer, put it into alcohol for ultrasonic cleaning for 10 minutes, and then put it into UV / Ozone for treatment 15 minutes incubation for 20 minutes. Dissolve 0.36g of EDC and 0.045g of NHS in 0.1M phosphate buffer (pH=6) as an activation solution. Immerse the transparent substrate in the activation solution for 5 hours to activate. After taking it out, wash it with ultrapure water and dry it with nitrogen. Sylgard 184 silicone rubber from Dow Corning Company was used to prepare ...

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Abstract

A kind of aflatoxin B1 rapid detection method based on smart phone detection system, described rapid detection method is to prepare aflatoxin B1 microchannel, and add detection reagent in the microchannel to make silver-stained PC sheet; after that through 3DMax software Design and print the aflatoxin B1 detection optical hardware by a 3D printer, place the silver-stained PC and the mobile phone in the optical hardware device, use the mobile phone camera to take pictures of the aflatoxin B1 reaction area, and process it through the smart phone detection program And display the test result of aflatoxin B1. The invention realizes the rapid detection of aflatoxin B1 based on the smart phone, has the advantages of convenient carrying, simple and convenient operation, low cost, high sensitivity, etc., and is suitable for rapid detection on the spot.

Description

technical field [0001] The invention relates to a rapid detection method for aflatoxin B1, specifically a method for adding aflatoxin B1 detection solution to polycarbonate (PC) and polydimethylsiloxane (PDMS) as materials In the multi-flux microchannel of the company, the silver-stained transparent plastic sheet is inserted into the hardware device, and the optical gray ratio (ODR) of the reaction band is calculated through the smart phone detection program to realize the analysis method of rapid quantitative detection of aflatoxin B1 . Background technique [0002] As the food industry enters a new stage, food safety issues have attracted worldwide attention. Among the many food safety risk factors, mycotoxin contamination is the most serious, among which aflatoxin B1 is the most toxic and carcinogenic. Traditional detection methods are mainly based on expensive large-scale instruments, such as high performance liquid chromatography, liquid chromatography-mass spectromet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N1/28G01N1/38H04M1/725H04M1/72403
CPCG01N1/28G01N1/38G01N21/84H04M1/72403
Inventor 李晓春杨帆张校亮于化忠
Owner TAIYUAN UNIV OF TECH
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