A rapid test method for aflatoxin b1 based on a smart phone detection system

Abstract

A kind of aflatoxin B1 rapid detection method based on smart phone detection system, described rapid detection method is to prepare aflatoxin B1 microchannel, and add detection reagent in the microchannel to make silver-stained PC sheet; after that through 3DMax software Design and print the aflatoxin B1 detection optical hardware by a 3D printer, place the silver-stained PC and the mobile phone in the optical hardware device, use the mobile phone camera to take pictures of the aflatoxin B1 reaction area, and process it through the smart phone detection program And display the test result of aflatoxin B1. The invention realizes the rapid detection of aflatoxin B1 based on the smart phone, has the advantages of convenient carrying, simple and convenient operation, low cost, high sensitivity, etc., and is suitable for rapid detection on the spot.

Description

technical field [0001] The invention relates to a rapid detection method for aflatoxin B1, specifically a method for adding aflatoxin B1 detection solution to polycarbonate (PC) and polydimethylsiloxane (PDMS) as materials In the multi-flux microchannel of the company, the silver-stained transparent plastic sheet is inserted into the hardware device, and the optical gray ratio (ODR) of the reaction band is calculated through the smart phone detection program to realize the analysis method of rapid quantitative detection of aflatoxin B1 . Background technique [0002] As the food industry enters a new stage, food safety issues have attracted worldwide attention. Among the many food safety risk factors, mycotoxin contamination is the most serious, among which aflatoxin B1 is the most toxic and carcinogenic. Traditional detection methods are mainly based on expensive large-scale instruments, such as high performance liquid chromatography, liquid chromatography-mass spectromet...

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Problems solved by technology

And Wong et al. (J. X. Wong, F. S. Liu and H-Z Yu, Mobile app-based quantitative scanometric analysis, Anal.Chem., 2014, 86, 11966-11971) have studied the quantitative detection by analyzing the optical gray scale ratio through the mobile phone application The purpose is to verify its performance by the reaction of biotin and streptavidin. Due to the limitation of the number of channels, it has certain limitations in multi-throughput detection and it only gives the optical gray scale ratio and cannot directly display the concentration value of the detected substance.

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