Method for detecting low density lipoprotein cholesterin through double-enzyme concerted catalysis silver deposition
A low-density lipoprotein and catalytic silver technology is applied in the field of double-enzyme synergistic catalytic silver deposition to detect low-density lipoprotein cholesterol. The effect of short detection times
Active Publication Date: 2016-07-20
GUILIN UNIV OF ELECTRONIC TECH
View PDF5 Cites 7 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0003] The purpose of the present invention is to provide a method for the detection of low-density lipoprotein cholesterol by co-catalyzing silver deposition with dual enzymes, which solves the problems of expensive, complicated operation, time-consuming and high technical requirements of the traditional LDL-C detection method
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0041] The present invention will be described in detail below in combination with specific embodiments. figure 1 It is a schematic diagram of an electrochemical method for detecting LDL-C based on the principle of dual-enzyme synergistically catalyzed silver deposition reaction. figure 2 Cyclic voltammetry characterization diagrams of different modification processes on the electrode surface; image 3 It is the AC impedance characterization diagram of different modification processes on the electrode surface. The implementation steps are as follows:
[0042] 1. Pretreatment of the electrode: use Al with a particle size of 0.3 μm and 0.05 μm 2 o 3 Grind and polish the surface of the glassy carbon electrode to a mirror surface with the suspension, and then wash the polished electrode with magnetic stirring in pure water and absolute ethanol for 5 minutes, rinse with pure water and then wash with magnetic stirring in pure water for 5 minutes; place the electrode in 30%H in ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a method for detecting low density lipoprotein cholesterin through double-enzyme concerted catalysis silver deposition. The method comprises the following steps: firstly, forming a sulfydryl-containing poly-aniline phenthiol film on an electrode surface through an electric polymerization method; performing electroreduction on gold ions on the electrode surface through a constant potential deposition method, thereby forming nanometer gold; anchoring the nanometer gold on the electrode surface through sulfydryl on an electric polymer membrane; fixing an apolipoprotein apoB-100 antibody on the nanometer gold; and via identification of the apoB-100 antibody on the specificity of low density lipoprotein, capturing the low density lipoprotein on the electrode surface. Under the synergistic effect of two enzymes of cholesterol esterase and cholesterol oxidase, cholesterol in the low density lipoprotein is decomposed and a weak reducing agent H2O2 is generated; the reducing agent can reduce and deposit silver ions on the gold nanometer grain surface; lastly, a standard curve is drawn by detecting a dissolving volt-ampere current value of an elemental silver, and the detection for the low density lipoprotein cholesterin can be realized.
Description
technical field [0001] The invention belongs to the technical field of biological detection, and relates to a method for detecting low-density lipoprotein cholesterol by synergistically catalyzing silver deposition with two enzymes. Background technique [0002] Low-density lipoprotein cholesterol (low-density lipoprotein cholesterol, LDL-C), is the main component of atherosclerotic plaque, is the culprit leading to atherosclerotic vascular disease. Clinical studies have confirmed that there is a positive correlation between elevated LDL-C and the incidence of atherosclerosis and coronary heart disease. Therefore, accurate determination of the content of LDL-C in serum is of great significance for the diagnosis, prevention and treatment of atherosclerosis, hypertension, coronary heart disease and other diseases. The detection methods mainly include ultracentrifugation, electrophoresis, chemical or immunoprecipitation, etc., but the precipitation method currently used in cli...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N27/26G01N27/48
CPCG01N27/26G01N27/48
Inventor 黄勇崔丽杰李桂银梁晋涛黄国银周治德白智昊
Owner GUILIN UNIV OF ELECTRONIC TECH