Preparation method and application of inhibition sensor for detection of 2,4-dichlorophenoxyacetic acid
A dichlorophenoxyacetic acid and sensor technology, applied in the direction of material electrochemical variables, etc., can solve the problems of complex sample pretreatment, toxic solvents, chemical hazards, etc., and achieve the effect of simple and fast detection, easy availability of raw materials, and low cost
Active Publication Date: 2018-10-19
YANGZHOU UNIV
View PDF4 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, if 2,4-d is used too much, due to its strong adsorption, the residual trace of the drug will cause certain harm. In order to determine the safety of food, people usually test and analyze the content of 2,4-d in plants
[0003] At present, the methods for detecting 2,4-d mainly include gas chromatography, high performance liquid chromatography, and gas chromatography. These methods have complex sample pretreatment, expensive instruments, toxic solvents, and require specially trained personnel to operate. and other defects
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0031] The technical solutions of the present invention will be further specifically described below through the embodiments and in conjunction with the accompanying drawings.
[0032] 1. Sensor preparation steps:
[0033] 1. Take 10.0 mg of calcium phosphate microspheres and disperse them in 2 mL of 0.5wt% chitosan, sonicate until completely dispersed to obtain a chitosan solution of 5 mg / mL calcium phosphate.
[0034] 2. Dissolve 16.0 mg of catalase in 2 mL of deionized water to obtain an enzyme solution with a concentration of 8 mg / mL of catalase.
[0035] 3. Take 50 μL of calcium phosphate chitosan solution and 50 μL of enzyme solvent, mix them, let stand for 24±5 hours to react, and obtain the composite material of catalase and calcium phosphate microspheres.
[0036] 4. Drop-coat 5 μL of the composite material of catalase and calcium phosphate microspheres on a clean glassy carbon electrode, and freeze-dry at 4°C to obtain the sensor.
[0037] 2. Make a linear relation...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
A method for preparing an inhibition sensor for 2,4-dichlorophenoxyacetic acid detection and its application, firstly dispersing calcium phosphate microspheres in chitosan, then immobilizing enzymes on the calcium phosphate microspheres, and finally The sensor is obtained by immobilizing the catalase and calcium phosphate microsphere complex on the glassy carbon electrode. The use of the present invention can overcome the shortcomings of traditional detection instruments such as expensive 2,4-d and the need for complicated sample pretreatment, greatly improving the performance of the sensor. The detection cost is reduced, and it is more convenient and quicker.
Description
technical field [0001] The invention relates to the technical field of preparation of enzyme sensors, in particular to an inhibition sensor based on catalase immobilized on calcium phosphate microspheres combined with flow injection to detect 2,4-dichlorophenoxyacetic acid (2,4-d) . Background technique [0002] 2,4-d (2,4-dichlorophenoxyacetic acid) is a major herbicide, used as a model compound for the study of microbial degradation of chlorinated aromatic compounds, and is also a synthetic plant hormone with Auxin (IAA) has similar physiological effects. 2,4-Dichlorophenoxyacetic acid herbicides, which can be used as plant growth regulators, are one of the commonly used auxin analogs for inducing callus formation. However, if 2,4-d is used too much, due to its strong adsorption, the residual trace of the drug will cause certain harm. In order to determine the safety of food, people usually test and analyze the content of 2,4-d in plants . [0003] At present, the meth...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/49
CPCG01N27/49
Inventor 徐琴刘凤平胡效亚
Owner YANGZHOU UNIV