A pyridine-degrading bacterium and its application in the treatment of pyridine-containing wastewater
A technology for pyridine-degrading bacteria and wastewater treatment, which is applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc. It can solve the problems of low pyridine tolerance concentration, limited number of pyridine-degrading strains, and single type of strains, etc. , to achieve the effect of high efficiency pyridine degradation ability and good application prospect
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Embodiment 1
[0015] Screening, isolation and identification of Pseudofulvimonas gallinarii NJUST27.
[0016] (1) Screening and isolation of strains
[0017] The mature aerobic granular sludge was taken out from the existing SBR reactor used to treat pyridine wastewater, thoroughly ground with a mortar, and then diluted to 10 with sterile water using a gradient dilution method. 4 -10 10 times. Prepare LB solid medium, pour it into the same sterilized 90×90cm petri dish while it is hot after sterilization, apply 20 μL of the previously diluted sample after cooling and solidification, and place it in a biochemical incubator at 30°C Culture 3d. The strains with obvious differences in colony characteristics on the petri dish were selected and purified by the method of plate streak separation. After three consecutive purifications, a single strain was obtained and stored on a slant. Pick the isolated single colonies, insert them into the inorganic salt medium MSM containing pyridine as the o...
Embodiment 2
[0029] Pyridine degradation performance of Pseudofulvimonas gallinarii NJUST27 strain.
[0030] The pyridine-degrading strain Pseudofulvimonas gallinarii NJUST27 was inoculated into LB medium supplemented with 1000 mg / L pyridine, and cultured on a shaker at a speed of 180 r / min at 30°C to enrich NJUST27 until the bacteria entered the late logarithmic growth phase ( About 3d), the obtained cells were centrifuged at 8000 rpm for 15 minutes, the supernatant was skimmed off, and the cells were resuspended in sterile liquid MSM by vortex shaking, and centrifuged. After repeating the washing process three times, the bacteria were resuspended in sterile liquid MSM (adjust the amount of MSM added to control the OD of the bacteria suspension) 600about 1.0) to obtain the seed solution. The LB medium used in the present invention is a conventionally used medium in the technical field.
[0031] Prepare liquid MSM containing 1000mg / L pyridine as simulated wastewater, add the above seed l...
Embodiment 3
[0034] Treatment of pyridine-containing industrial wastewater by Pseudofulvimonas gallinarii NJUST27.
[0035] Pseudofulvimonas gallinarii NJUST27 seed liquid is inserted into the actual industrial waste water containing pyridine (containing pyridine 989mg / L, COD1328mg / L, total phosphorus 55mg / L) with 5% inoculum amount, shakes with the rotating speed of 180r / min under the condition of 30 ℃. bed culture. Monitor the COD value, pyridine and ammonia nitrogen concentration before and after wastewater treatment.
[0036] It can be seen from Table 2 that after the pyridine-containing industrial wastewater was treated by the strain Pseudofulvimonas gallinarii NJUST27 for 98 hours, the pyridine was completely degraded, the COD value was reduced to 168 mg / L, and the ammonia nitrogen release was 43.54 mg / L. The pyridine degradation rate, COD removal rate and ammonia nitrogen conversion rate were 100%, 87.35% and 24.57%, respectively.
[0037] This example shows that the isolated Pseu...
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