Method for improving huperzia serrata explant surface sterilization efficiency

A technology of surface sterilization and thousand-layer tower, applied in the biological field, can solve the problems of difficulty in sterilization of explants, inability to grow, differentiate, proliferate, and die, and achieve the effect of improving the survival rate of transplanting and improving the efficiency of sterilization.

Inactive Publication Date: 2016-08-03
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These explants have problems such as difficulty in explant sterilization, low rate of sterile explant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1 (Control 1):

[0030] 1. Step 1. Sterilize the explants of the Melaleuca Tower:

[0031] Take the Melaleuca leaves, rinse them with running water, soak them in a 75% ethanol solution for 1 minute, pour out the ethanol, and then soak them in a 5% NaOCl solution for 15 minutes, then take them out, pour out the NaOCl solution and replace Soak in hydrogen peroxide solution with a volume fraction of 7%, leave it for 10 minutes and take it out, pour out the hydrogen peroxide solution, and rinse with sterile water;

[0032] Step two, the cultivation of the explants of Melaleuca Tower:

[0033] The sterilized Melaleuca leaves were transferred to MS solid medium containing 150 mg / L amphotericin B, and then placed in the culture room for culture. The culture conditions are as follows: temperature is 25℃, light intensity is 2000 Lux, photoperiod is 16 hours. Two weeks later, the sterilization rate and survival rate of explants were 26% and 43%, respectively.

Example Embodiment

[0034] Example 2 (Control 2):

[0035] If the explants in Example 1 were replaced with stems, the sterilization rate and survival rate were 30% and 52%, respectively.

Example Embodiment

[0036] Example 3 (Control 3):

[0037] If the explants in Example 1 were replaced with roots, the sterilization rate and survival rate were 47% and 25%, respectively.

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PUM

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Abstract

The invention discloses a method for improving huperzia serrata explant surface sterilization efficiency.The method includes the steps that a huperzia serrata explant is sterilized through a sterilization agent in the low-pressure environment, and the sterilized huperzia serrata explant is cultured in the low-pressure environment, wherein the low-pressure environment refers to the environment with the pressure of 40-60 kP.By means of the treatment method combining low pressure and high temperature, sterilization efficiency of the huperzia serrata explant surface is improved.Meanwhile, the survival rate of the huperzia serrata explant is increased.The method is easy to operate, sterilization efficiency is improved on the premise that later culture of huperzia serrata is not affected, and the method can be used for tissue culture of huperzia serrata.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving the surface sterilization efficiency of Huperziaserrata (Thunb.) Trev. explants. Background technique [0002] Melaleuca (Huperziaserrata (Thunb.) Trev.) is a perennial herbaceous fern of Lycopodiaceae (Lycopodiaceae) in the order Lycopodiales. Melaleuca pagoda was first recorded in "Plant Names and Facts", and its nature and flavor are hard and flat. It has the effects of dissipating blood stasis, reducing swelling, detoxifying and relieving pain. In 1927, Chinese scientific and technological workers reported that huperzine A isolated from this plant has the effect of relaxing striated muscles. Afterwards, a large number of studies found that huperzine A is a highly efficient, low toxicity, reversible and highly selective inhibitor of acetylcholinesterase. agent. Now, it is used as a drug for the treatment of Alzheimer's disease (AD, Alzheimer's...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 郭斌任岩岩何美娜何玮傅艳萍尉亚辉
Owner NORTHWEST UNIV
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