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Method for improving huperzia serrata explant surface sterilization efficiency

A technology of surface sterilization and thousand-layer tower, applied in the biological field, can solve the problems of difficulty in sterilization of explants, inability to grow, differentiate, proliferate, and die, and achieve the effect of improving the survival rate of transplanting and improving the efficiency of sterilization.

Inactive Publication Date: 2016-08-03
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These explants have problems such as difficulty in explant sterilization, low rate of sterile explant acquisition, and most of them die after sterilization, unable to grow, differentiate and proliferate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 (contrast 1):

[0030] 1. Step 1, the sterilization of the Melaleuca explants:

[0031] Take the leaf of the Melaleuca tower, rinse it with running water, soak it with a 75% ethanol solution for 1 minute, pour off the ethanol, then soak it with a 5% NaOCl solution for 15 minutes, take it out, pour off the NaOCl solution, and replace it. Soak it in a hydrogen peroxide solution with a volume fraction of 7%, take it out after standing for 10 minutes, pour off the hydrogen peroxide solution, and rinse it with sterile water;

[0032] Step 2, cultivation of Melaleuca explants:

[0033] The sterilized Melaleuca leaves were transferred to MS solid medium containing 150 mg / L amphotericin B, and then placed in a culture room for cultivation. The cultivation conditions are as follows: the temperature is 25°C, the light intensity is 2000 Lux, and the photoperiod is 16 hours. After 2 weeks, the bacteria removal rate and survival rate of the explants were calculated t...

Embodiment 2

[0034] Embodiment 2 (contrast 2):

[0035] In Example 1, explants are replaced with stems, and the bacteria removal rate and survival rate are 30% and 52% respectively.

Embodiment 3

[0036] Embodiment 3 (contrast 3):

[0037] Replace the explants in Example 1 with roots, the bacteria removal rate and survival rate are 47% and 25% respectively.

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PUM

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Abstract

The invention discloses a method for improving huperzia serrata explant surface sterilization efficiency.The method includes the steps that a huperzia serrata explant is sterilized through a sterilization agent in the low-pressure environment, and the sterilized huperzia serrata explant is cultured in the low-pressure environment, wherein the low-pressure environment refers to the environment with the pressure of 40-60 kP.By means of the treatment method combining low pressure and high temperature, sterilization efficiency of the huperzia serrata explant surface is improved.Meanwhile, the survival rate of the huperzia serrata explant is increased.The method is easy to operate, sterilization efficiency is improved on the premise that later culture of huperzia serrata is not affected, and the method can be used for tissue culture of huperzia serrata.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving the surface sterilization efficiency of Huperziaserrata (Thunb.) Trev. explants. Background technique [0002] Melaleuca (Huperziaserrata (Thunb.) Trev.) is a perennial herbaceous fern of Lycopodiaceae (Lycopodiaceae) in the order Lycopodiales. Melaleuca pagoda was first recorded in "Plant Names and Facts", and its nature and flavor are hard and flat. It has the effects of dissipating blood stasis, reducing swelling, detoxifying and relieving pain. In 1927, Chinese scientific and technological workers reported that huperzine A isolated from this plant has the effect of relaxing striated muscles. Afterwards, a large number of studies found that huperzine A is a highly efficient, low toxicity, reversible and highly selective inhibitor of acetylcholinesterase. agent. Now, it is used as a drug for the treatment of Alzheimer's disease (AD, Alzheimer's...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 郭斌任岩岩何美娜何玮傅艳萍尉亚辉
Owner NORTHWEST UNIV
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