Bacillus and application thereof to production of acetoin and 2, 3- butanediol through high temperature fermentation

Abstract

The invention discloses bacillus and application thereof to production of acetoin and 2, 3- butanediol through high temperature fermentation. A strain is Bacillus sp. H15-1CGMCC No. 12389, the gram faerbung is positive, spores are produced, the cellular morphology is in a rod shape, and the stain can grow at the temperature of 40-60 DEG C and proliferate at the highest speed at the temperature of 50 DEG C. By means of the strain, degerminated corn flour hydrolysate is adopted as the main raw material, a mechanical stirring and ventilation fermentation tank is adopted for conducting fermentation for 68 hours at the temperature of 50 DEG C, and acetoin with the concentration being 50.8 g / L and 2, 3- butanediol with the concentration being 32.1 g / L can be generated. A method for producing acetoin and 2, 3- butanediol through high temperature fermentation has the advantages that raw materials are low in cost, the fermentation process is resistant to infectious microbe contamination, cooling water is saved, and operation is conducted coarsely.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacillus strain and its application. Background technique [0002] Acetoin is 3-hydroxy-2-butanone, which has important uses in food, fragrance, cosmetics, chiral synthesis and other fields (Xiao and Lu, 2014). 2,3-Butanediol has important uses in chemical synthesis, food, fuel, antifreeze and other fields (Jie et al., 2011). Acetoin and 2,3-butanediol can be fermented from renewable raw materials and are important emerging platform compounds (Xue et al., 2012). The two are the products of the acetoin metabolic pathway in some bacteria, and can be transformed into each other through the action of 2,3-butanediol dehydrogenase in the cell. Acetoin and 2,3-butanediol often exist together in fermentation products, but their boiling points are quite different, and they can be easily separated from each other by distillation. [0003] The production of acetoin and 2,3-buta...

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Problems solved by technology

[0005] So far, there are very few research reports on the production of acetoin and 2,3-butanediol by high-temperature fermentation at home and abroad. The research group of the inventor once used a strain of Geobacillus bacteria to produce these two products by high-temperature fermentation (Xiaoetal., 2012), and Authorized 1 Chinese invention patent (ZL201210060900.6), but the fermentation medium of this method uses raw materials such as glucose, peptone, yeast powder, etc., and the cost is very high, which is not suitable for low-cost, large-scale industrial production

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