A method of promoting napa cabbage microspore embryogeny and direct production of plantlets

A technology for culturing microspores and microspores, which is applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of labor-intensive, low embryoid seedling rate, low embryogenesis rate, etc., and achieves simple operation. easy effect

Inactive Publication Date: 2016-08-10
SHENYANG AGRI UNIV
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Problems solved by technology

To take advantage of dominant hybrid breeding, the parents must be purified first. The traditional continuous selfing method takes a long time, which is labor-intensive and labor-intensive; it only takes one year to obtain homozygotes using the free microspore culture method, which saves time and saves time. The workload is reduced, and the regenerated plants cultivated in this way can not only create new breeding materials, but also have strong intergenerational stabi...

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  • A method of promoting napa cabbage microspore embryogeny and direct production of plantlets
  • A method of promoting napa cabbage microspore embryogeny and direct production of plantlets
  • A method of promoting napa cabbage microspore embryogeny and direct production of plantlets

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Embodiment Construction

[0019] The methods described in the following examples are conventional methods unless otherwise specified.

[0020] The reagents described in the following examples can be obtained from commercial sources unless otherwise specified.

[0021] The medium used in the following examples is the same as the B5, NLN, and MS medium in the summary of the invention.

[0022] The cabbage microspore culture method main process of the present invention comprises:

[0023] 1. Free microspore culture process

[0024] Select the flower buds whose microspore development period of Chinese cabbage is from the uninucleate marginal stage to the early binucleate stage. At this time, the characteristics of the flower buds are that the ratio of the length of the petals to the length of the anthers is between 1 / 2 and 3 / 4. After washing the flower buds with running water for 2 to 3 times , put the flower buds into a sterilized 100mL beaker, sterilize the surface with 75% ethanol solution for 30s, 0....

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Abstract

A method of promoting napa cabbage microspore embryogeny and direct production of plantlets is provided to overcome the problem that microspore culturing techniques at present are low in microspore embryogeny rate, low in the rate of direct production of plantlets and long in culturing period. Through adding a histone deacetylase inhibitor-trichostatin A (TSA) into an induction medium, the microspore embryogeny rate and the rate of direct production of plantlets of napa cabbage are increased. In an NLN induction medium to which 0.025-0.10 muM of the TSA is added, the microspore embryogeny rate of the napa cabbage is increased by 1.46-2.48 times, and the rate of direct production of plantlets of the napa cabbage is increased by 1.12-1.39 times. The medium is a liquid mixture consisting of the NLN induction medium, the 0.025-0.10 muM of the TSA, 130 g.L<-1> of cane sugar, 100 muL of sterilized agarose having a concentration of 0.5 g.L<-1> and 10 g.L<-1> of activated carbon.

Description

technical field [0001] The invention relates to a method for culturing free microspores of Chinese cabbage, belongs to the technical field of cell breeding, and is a method for promoting embryogenesis and direct seedling formation of Chinese cabbage microspores. Background technique [0002] Chinese cabbage (Brassica rapa. ssp. chinensis L.) is a typical cross-pollinated plant of Brassicaceae, which has significant heterosis. To take advantage of dominant hybrid breeding, the parents must be purified first. The traditional continuous selfing method takes a long time, which is labor-intensive and labor-intensive; it only takes one year to obtain homozygotes using the free microspore culture method, which saves time and saves time. The workload is reduced, and the regenerated plants cultivated in this way can not only create new breeding materials, but also have strong intergenerational stability and strong heterosis. In recent decades, some researchers at home and abroad hav...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 冯辉张露章云唐小燕刘志勇李承彧冀瑞琴王玉刚
Owner SHENYANG AGRI UNIV
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