Bone cell LIMK2 gene and novel application of expression products of bone cell LIMK2 gene

A technology for gene expression and expression products, applied in the field of medicine, can solve the problems of new bone tissue being unable to withstand external force and long time for bone remodeling, so as to reduce the impact and promote proliferation.

Inactive Publication Date: 2016-08-10
付强
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] On the one hand, the object of the present invention is to overcome the shortcomings of the prior art that the bone remodeling time is too long and the new bone tissue cannot withstand external forces in a short period of time, and provide bone cell LIMK2 gene and its expression products for the treatment and bone remodeling process. Drug application in related diseases

Method used

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  • Bone cell LIMK2 gene and novel application of expression products of bone cell LIMK2 gene
  • Bone cell LIMK2 gene and novel application of expression products of bone cell LIMK2 gene
  • Bone cell LIMK2 gene and novel application of expression products of bone cell LIMK2 gene

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Effect test

Embodiment 1

[0045] RNA interference of LIMK2 gene in MLO-Y4 osteoid cell line and its effect on the flow of MLO-Y4 osteoid cell line Effects of cytoskeletal remodeling under body shear stress

[0046] 1. Materials:

[0047] 1.1. Experimental cells:

[0048] P35 MLO-Y4 osteoid cell line, a gift from Professor Lynda F Bonewald of the United States.

[0049] 1.2. Main experimental instruments:

[0050]

[0051] 1.3. Main reagents and consumables:

[0052]

[0053]

[0054]

[0055]1.4. Main solution preparation:

[0056] 1) α-MEM medium containing 5% serum: a bag of α-MEM powder (g), NaHCO 3 2.2g, add ddH 2 O was fixed to 1L, stirred with a magnetic stirrer for 60min, adjusted to pH 7.2, sterilized by filtration, added heat-inactivated fetal bovine serum (FBS-2.5%) and calf serum (CS-2.5%) to prepare the serum content 5% medium.

[0057] 2) DPBS buffer: a bag of DPBS powder (g), add ddH 2 O was adjusted to 1L, and sterilized under high temperature and high pressure. ...

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Abstract

The invention discloses a bone cell LIMK2 gene and application of expression products of the bone cell LIMK2 gene. The expression products can be applied to preparing medicines for treating diseases related to bone remodeling processes. The bone cell LIMK2 gene and the application have the advantages that the bone cell LIMK2 gene and / or the expression products of the bone cell LIMK2 gene are inhibited, so that remodeling of cytoskeletons of bone cells under the effects of fluid shear force can be inhibited, the mechanical sensitivity of the bone cells can be directly and specifically subjected to up-regulation, and proliferation of the bone cells can be promoted; functions of osteoblasts and osteoclasts can be regulated and controlled by the bone cells, accordingly, the bone remodeling speed and balance can be controlled, the bone remodeling processes can be ultimately accelerated, and repair and reconstruction procedures for diseases such as bone fracture, osteoporosis, bone defect diseases, dysosteogenesis and bone malformation can be accelerated.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to the application of bone cell LIMK2 gene and its expression product in the preparation of medicines for treating diseases related to bone remodeling process. Background technique [0002] Bone remodeling is a microscopic process that must be experienced in the repair and reconstruction of fractures, bone defects and other diseases. New bone tissue must undergo bone remodeling to become mature bone tissue before it can withstand external forces. At present, the treatment methods for diseases such as fractures and bone defects all have the disadvantages that the bone remodeling time is too long, and the new bone tissue cannot withstand external forces in a short period of time. Whether bone grafting (using autologous bone, allogeneic bone or artificial bone substitutes) or distraction osteogenesis or bone tissue engineering is used for the repair and reconstruction of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K48/00A61K31/713C12N15/113A61P19/08A61P19/10A61P1/02
CPCA61K31/713A61K45/00A61K48/005C12N15/113C12N2310/141
Inventor 付强谭淑仪宋子珺黄春煌任林
Owner 付强
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