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Method for chemically degrading high polymerization proanthocyanidins by means of sulfurous acid

A high-polymerization proanthocyanidin and proanthocyanidin technology, applied in the field of bioengineering, can solve the problems of long production cycle, low product yield and high cost, and achieve the effect of reducing production cost

Inactive Publication Date: 2016-08-10
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention solves the problems that the preparation process of oligomeric proanthocyanidins in the prior art needs to use a large amount of organic solvents and resins, the cost is high, the production cycle is long, the operation is complicated, and the product yield is low, and further provides a simple operation, production Short cycle, green environmental protection, recyclable chemical reagents, practical preparation method of oligomeric proanthocyanidins

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0033] The preparation method of the oligomeric proanthocyanidins described in this embodiment comprises the following steps:

[0034] (1) Reconstitute 1Kg of proanthocyanidin crude extract (proanthocyanidin content 82%, average degree of polymerization 5.8, Huludao Maohua Biological Co., Ltd.) with 3L 45°C warm water to obtain proanthocyanidin suspension;

[0035] (2) Put the proanthocyanidin suspension into a 10L stainless steel bucket, control the temperature in the bucket at 50°C, add a total volume of 200ml sulfurous acid solution (AR, SO 2 ≥6%), the adding speed was controlled within 10ml per minute at the beginning, and the adding was completed within 10 minutes. The stirring reaction was continued for 1 hour.

[0036] (3) Weigh 200g (0.2mol) of calcium carbonate, grind it properly into powder, filter it through a 200-mesh stainless steel screen, sprinkle the powder evenly into the container, and stir it for 2 minutes before sprinkling once (about 10g) For the second ...

Embodiment 2

[0041] The preparation method of the oligomeric proanthocyanidins described in this embodiment comprises the following steps:

[0042] (1) Reconstitute 1Kg of proanthocyanidin crude extract (purple sweet potato anthocyanin raw material extracted by our unit, purple sweet potato comes from Xuzhou Academy of Agricultural Sciences, the average degree of polymerization is 8.4) with 3L of 50℃ warm water to obtain proanthocyanidin suspension ;

[0043] (2) Put the proanthocyanidin suspension into a 10L stainless steel bucket, control the temperature in the bucket at 50°C, add a total volume of 200ml sulfurous acid solution (AR, SO 2 ≥6%), the adding speed was controlled within 10ml per minute at the beginning, and the adding was completed within 10 minutes. The stirring reaction was continued for 1.5 hours.

[0044] (3) Weigh 200g (0.2mol) of calcium carbonate, grind it properly into powder, filter it through a 200-mesh stainless steel screen, sprinkle the powder evenly into the c...

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Abstract

The invention discloses a method for chemical reduction and high polymerization of proanthocyanidins. The method utilizes the characteristic that sulfurous acid can degrade proanthocyanidins with a high degree of polymerization, and completes the conversion of proanthocyanidins from a high-polymerization state to an oligomerization state. The added sulfurous acid is precipitated by excessive calcium carbonate powder to generate another precipitated calcium sulfite. The precipitates are centrifuged or filtered to obtain oligomeric proanthocyanidins with a degree of polymerization of 2-3. The method simplifies the process steps, uses no organic solvent in the whole reaction, has extremely low requirements on equipment investment and workers' operation, is suitable for industrial production, and is a green chemical method that meets environmental requirements. The proanthocyanidin high polymer can be degraded into the proanthocyanidin oligomer, which increases the content of the oligomer in the raw material, and can ensure that the original components in the raw material remain unchanged to the greatest extent.

Description

technical field [0001] The invention relates to a production method for obtaining oligomeric proanthocyanidins by sulfurous acid treatment, and belongs to the technical field of bioengineering. Background technique [0002] Proanthocyanidins, also known as condensed tannins, are polymers formed by the condensation of flavans. It is formed by condensation of different amounts of catechin or epicatechin, forming dimers to dozens of polymer compounds, according to the degree of polymerization. Size, 2-4 polymers are usually referred to as oligomeric proanthocyanidins, and those with 5 polymers or more are called high polymer proanthocyanidins. [0003] The biological activity of proanthocyanidins is related to the degree of polymerization of tannins. Previous studies on the biological activities of proanthocyanidins from other sources have shown that when the degree of polymerization is greater than 3, the solubility and antioxidant capacity of proanthocyanidins decrease with t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 陈才法郭璨李长峰杨茂钱郁和沈洁周郑坤郑元林
Owner XUZHOU NORMAL UNIVERSITY
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