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A hierarchically ordered macroporous-mesoporous silica monolithic column bovine serum albumin imprinted polymer and its preparation method

A technology of serum albumin and imprinted polymers, applied in chemical instruments and methods, and other chemical processes, can solve the problems of large protein molecular size, difficult mass transfer, lack of selectivity, etc., and achieve strong permeability and mass transfer Fast kinetics, effect of improving selectivity

Active Publication Date: 2018-01-16
上海健士拜生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Western blotting polymers are rarely reported, especially macromolecular western blotting polymers
Proteins have large molecular sizes and complex conformations, making mass transfer difficult and lacking in selectivity

Method used

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  • A hierarchically ordered macroporous-mesoporous silica monolithic column bovine serum albumin imprinted polymer and its preparation method
  • A hierarchically ordered macroporous-mesoporous silica monolithic column bovine serum albumin imprinted polymer and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Dissolve 0.4g polystyrene in 8mL water, sonicate the solution for 20min, settle naturally for a week, carefully remove the upper liquid, and vacuum dry at 25°C for 48h to obtain polystyrene colloidal crystals.

[0036] (2) 2g TEOS, 0.9g water, 0.8g hydrochloric acid, 1.0g P123 and 15g absolute ethanol were prepared as a precursor solution, 0.4g polystyrene colloidal crystals were added to the solution, immersed for 1h, and the concentration of hydrochloric acid was 2mol / L. Then soak and evaporate at 30°C for 48h. The synthesized bulk material was placed in a tube furnace and heated in air at 1 °C min -1The temperature was raised slowly at a rate of 550° C. for 6 hours to obtain a hierarchically ordered macroporous-mesoporous silica monolithic column (HOM).

[0037] (3) 0.4 g of the HOM obtained in step (2) was dissolved in 40 mL of absolute ethanol. Then 2mL of MPS was added to the mixed solution, and reacted at 40°C for 12h. After the reaction, the product was...

Embodiment 2

[0042] (1) Dissolve 0.8g polystyrene in 8mL water, sonicate the solution for 20min, settle naturally for a week, carefully remove the upper liquid, and vacuum dry at 25°C for 48h to obtain polystyrene colloidal crystals.

[0043] (2) 1.5g TEOS, 0.9g water, 0.1g hydrochloric acid, 1.0g P123 and 15g absolute ethanol were prepared as a precursor solution, 0.6g polystyrene colloidal crystals were added to the solution, immersed for 1h, and the concentration of hydrochloric acid was 2mol / L. Then soak and evaporate at 30°C for 48h. The synthesized bulk material was placed in a tube furnace and heated in air at 1 °C min -1 The temperature was raised slowly at a rate of 550° C. for 6 hours to obtain a hierarchically ordered macroporous-mesoporous silica monolithic column (HOM).

[0044] (3) 0.4 g of the HOM obtained in step (2) was dissolved in 40 mL of absolute ethanol. Then 2mL of MPS was added to the mixed solution, and reacted at 40°C for 12h. After the reaction, the product ...

Embodiment 3

[0047] (1) 1.0g polystyrene was dissolved in 8mL water, the solution was ultrasonicated for 20min, allowed to settle naturally for a week, the upper liquid was carefully removed, and vacuum-dried at 25°C for 48h to obtain polystyrene colloidal crystals.

[0048] (2) 2.5g TEOS, 0.9g water, 0.4g hydrochloric acid, 1.0g P123 and 15g absolute ethanol are prepared as a precursor solution, 1.0g polystyrene colloidal crystals are added to the solution, immersed for 1h, and the concentration of hydrochloric acid is 2mol / L. Then soak and evaporate at 30°C for 48h. The synthesized bulk material was placed in a tube furnace and heated in air at 1 °C min -1 The temperature was raised slowly at a rate of 550° C. for 6 hours to obtain a hierarchically ordered macroporous-mesoporous silica monolithic column (HOM).

[0049] (3) 0.4 g of the HOM obtained in step (2) was dissolved in 40 mL of absolute ethanol. Then 2mL of MPS was added to the mixed solution, and reacted at 40°C for 12h. Af...

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Abstract

The invention provides a hierarchical ordered macroporous-mesoporous monolithic silica column bovine serum albumin imprinted polymer and a preparation method thereof. The preparation method comprises the following steps: synthesis of polystyrene colloidal crystal; synthesis of a hierarchical ordered macroporous-mesoporous monolithic silica column; preparation of gamma-(methyacryloyloxy)propyltrimethoxysilane modification material for the hierarchical ordered macroporous-mesoporous monolithic silica column; and preparation of the hierarchical ordered macroporous-mesoporous monolithic silica column bovine serum albumin imprinted polymer. The synthesized hierarchical ordered macroporous-mesoporous monolithic silica column has regular macroscopic and microscopic structures, good permeability, low backpressure and fast mass transfer kinetic action; the recognition site of the synthesized bovine serum albumin imprinted polymer is located on or close to the surface of the polymer, which facilitates bovine serum albumin molecules in entering and exiting from imprinting holes and improves selectivity of bovine serum albumin; and the hierarchical ordered macroporous-mesoporous monolithic silica column bovine serum albumin imprinted polymer has good bovine serum albumin separation performance and has adsorption capacity of 121.6 mg / g.

Description

technical field [0001] The invention relates to a silica gel monolithic column imprinted polymer, in particular to a hierarchically ordered porous silica gel monolithic column imprinted polymer and a preparation method thereof, belonging to the technical fields of material preparation and bioengineering. Background technique [0002] Solid-phase extraction (Solid-Phase Extraction, SPE) is a sample pretreatment technology developed in recent years, which is mainly used for the separation and purification of samples. It is easy to operate, saves time and effort, and can more effectively separate analytes from interfering components, and is widely used in medicine, food, chemical and other fields. Separation columns for solid phase extraction are usually packed with particles of controlled size. The smaller the particle diameter, the higher the separation efficiency, but the back pressure also increases. Recently, the concept of monolithic columns has been introduced into sep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F222/38C08F220/56C08J9/26B01J20/26B01J20/30
CPCB01J20/268C08F222/385C08J9/26C08J2201/042C08J2335/00C08F220/56
Inventor 刘燕刘方方倪良孟敏佳钟国星
Owner 上海健士拜生物科技有限公司