Cell culture dish and cell sampling method thereof
A cell culture and petri dish technology, which is applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problem of difficult screening of monoclonal cells, reduced screening success rate, low screening efficiency, etc. problem, to achieve the effect of improving the success rate of the experiment, shortening the experiment cycle, and improving efficiency
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Embodiment 1
[0042] figure 1 It is a schematic diagram of the structure of a single well of a culture dish. The culture dish has various specifications and sizes, and there are 6 holes, 12 holes, 24 holes, 48 holes, 96 holes and other specifications in terms of the number of culture holes. figure 1 Only one of the Petri dish wells is shown in . Such as figure 1 and figure 2 As shown, there are multiple concentric circles 13 and straight lines 12 passing through the centers of the concentric circles at the bottom of a single culture dish hole 10, and the concentric circles 13 and the straight lines 12 together form a positioning line. Such as figure 2 As shown, the bottom of the petri dish is divided into multiple regions by the concentric circles and cross lines. Assuming that the area of the bottom surface of the petri dish is S, and assuming that the radii of the three concentric circles are a, b, and c respectively, the following relationship is satisfied: c = 2a, The radius...
Embodiment 2
[0056] The petri dish in this embodiment is basically the same as that in the first embodiment, except that the lengths of the protrusions and grooves on the holes of the petri dish and the sleeve are different from those in the first embodiment. In this embodiment, the first set of barrels is taken as an example for illustration, and the grooves and protrusions of the other sets of barrels are consistent with those of the first set of barrels.
[0057] Such as Figure 9 and Figure 10 As shown, the longitudinal protrusion 51 on the culture dish hole 50 does not completely penetrate the hole wall, that is to say, there is no protrusion structure on the upper part of the hole wall of the culture well 50 . The longitudinal groove 61 on the first set of barrels 60 runs through the barrel wall up and down, or is only distributed in the lower half of the set of barrels. There is no protruding structure in part, and the two can rotate relatively, and the position of the sampling h...
Embodiment 3
[0065] The culture dish and the sleeve in this embodiment can adopt the structure in Embodiment 1 or Embodiment 2.
[0066] The difference between this embodiment and the previous two embodiments is that there is a ring of protruding edges at the lower openings of the first sampling hole, the second sampling hole and the third sampling hole. Taking the third set of barrels 40 as an example, as Figure 11 As shown, the third sampling hole 41 extends downwards from a raised edge 43 . The protruding edges of the three sampling holes are used as supports to prevent the bottom of the barrel from damaging cells outside the sampling holes. At the same time, this ring of protrusions can separate the selected cell area from other areas. Prevent cell digestion fluid or cells from leaking out.
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