A method for producing exopolysaccharide by using Rhodospira exothionibacterium
A technology for exothiorhodospirillum and exopolysaccharide, which is applied in the field of utilizing exothiorhodospirillum to produce exopolysaccharide, and can solve the problems of low yield, unseen problems and the like
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[0033]The preparation method of exopolysaccharides involved in the present invention can be understood as a method for producing exopolysaccharides using Rhodospira exothionibacterium, which includes the step of inoculating the fermentation strain into the fermentation medium for light fermentation and culture, wherein, The fermentation strains are obtained from corresponding strains through seed culture.
[0034] As known to those skilled in the art, at present, 16S Ribosomal RNA (16S rRNA) is often used internationally for molecular identification of bacteria, so 16S rRNA can be used for comparison of similarities to obtain their homology. Therefore, the fermentation strains used in the present invention are not limited to the field isolates used in the present invention. The 16s rRNA gene is the DNA sequence corresponding to the rRNA encoded on the bacterial chromosome, and exists in the chromosomal genome of all bacteria. figure 1 The 16S rDNA-based molecular phylogenetic ...
Embodiment 1
[0075] Example 1: To investigate the effects of different carbon sources on the optical density and exopolysaccharide content of fermentation cultures.
[0076] 1. Preparation of Seed Solution
[0077] (1) Preparation of liquid medium: The liquid medium used for seed culture is calculated in 1L of water, including the following components in 1L of water:
[0078]
[0079] (2) Inoculation and seed culture: Pick and screen the monoclonal colonies of Rhodospira exothionibacterium LBD strain and inoculate them into 10ml of the above-mentioned liquid medium, and cultivate them for 5 days at a temperature of 30-35°C and a light of 2000-8000Lx. The first-generation fermented strains obtained were transferred to 50ml liquid medium again, and cultured for 5 days under the same conditions to obtain the second-generation fermented strains (seed liquid) for future use.
[0080] 2. Preparation of fermentation medium
[0081] (1) Prepare the fermentation medium, the fermentation medium i...
Embodiment 2
[0088] Example 2: Industrialized cultivation of Rhodospira exothias LBD strain.
[0089] The preparation of seed solution and the separation and purification process of exopolysaccharide in Example 2 are the same as in Example 1.
[0090] Fermentation medium in embodiment 2 is calculated by 1L water, comprises the following components in 1L water:
[0091]
[0092] Adjust the initial pH value to 7.2 with sodium carbonate and hydrochloric acid solution, fill 2 L of the prepared sterilized liquid medium into a 2.5 L culture bottle, and insert the fermentation strain (seed liquid) into it.
[0093] The optical density of the culture is OD 500nm Can reach 5 or more. Using this medium to culture Rhodospirillum exothioni LBD strain for 120 hours, the optical density of the fermentation culture was OD 500nm It can reach more than 5, and the concentration of exopolysaccharide in the fermentation culture after filtering through a 0.45 μ filter membrane to remove bacteria can reac...
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Abstract
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