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A microfluidic chemiluminescent immunoassay device and its application method

A technology of chemiluminescence immunoassay and detection device, which is applied in the field of microfluidic chemiluminescence immunoassay device, can solve the problems of complicated steps and time-consuming of chemiluminescence immunoassay.

Active Publication Date: 2017-12-22
ZHEJIANG PUSHKANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The steps of chemiluminescent immunoassay are complicated and time-consuming: (1) It is necessary to add capture antibody, antigen, detection antibody linked to enzyme, cleaning solution, and substrate one after another

Method used

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  • A microfluidic chemiluminescent immunoassay device and its application method
  • A microfluidic chemiluminescent immunoassay device and its application method
  • A microfluidic chemiluminescent immunoassay device and its application method

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Embodiment 1

[0026] A microfluidic chemiluminescent immunoassay device of this embodiment, such as figure 1 , 2As shown, it includes a microfluidic disk 13, and 12 microfluidic detection units are installed on the microfluidic disk 13 (belonging to the single-pointer multi-person operation); the microfluidic detection unit includes a whole blood injection tank 1. The whole blood injection tank 1 is connected to the blood cell storage tank 3 through the whole blood separation channel 2, and the blood cell storage tank 3 is connected to the plasma delivery channel 4; the plasma delivery channel 4 is connected to the mixing / detection channel The top of the tank 7 is connected; the top of the mixing / detection tank 7 is communicated with the first reagent storage tank 9 through a pipeline; the top of the mixing / detection tank 7 is also communicated with the third reagent storage tank 10 through a pipeline; A gas compression release tank 8 is installed on one side of the mixing / detecting tank 7...

Embodiment 2

[0030] A microfluidic chemiluminescent immunoassay device of this embodiment, such as image 3 , 4 As shown, the microfluidic disk 13 is included, and the microfluidic disk 13 is equipped with 4 microfluidic detection units, (each detection unit can simultaneously complete three immune indicators, and can simultaneously perform at least 4 sample detections, The microfluidic detection unit that belongs to the small set meal unit for one person) includes a whole blood injection tank 1, and the whole blood injection tank 1 is connected with a blood cell storage tank 3, and the blood cell storage tank 3 is connected with a plasma transmission flow channel 4 The plasma transmission channel 4 is connected to the top of the mixing / detecting tank 7; the top of the mixing / detecting tank 7 is communicated with the first reagent storage tank 9 through a pipeline; the mixing / detecting tank 7 The top is also communicated with the third reagent storage tank 10 through a pipeline; a gas com...

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Abstract

The invention discloses a microfluidic chemiluminescence immune detection device and a use method thereof. The microfluidic chemiluminescence immune detection device comprises a microfluidic disc which is provided with a microflow detecting unit, the microflow detecting unit comprises a whole blood injection tank, a whole blood separation channel, a blood cell storage tank, a plasma conveying runner, a mixing / detecting tank, a first reagent storage tank, a cleaning liquid injection tank, a gas compressing and releasing tank, a waste liquid tank, a second reagent storage tank and a fourth reagent storage tank. Micro fluid in the microfluidic disc can be intelligently controlled through a simple method, meanwhile, reaction liquid can be fully mixed, it is guaranteed that a reaction system is carried out efficiently, and quantitative detection of hCG concentration in a sample is fast achieved. The advantages of being easy to operate, high in detecting sensitivity, accurate and reliable in result, good in repeatability and low in cost are achieved.

Description

technical field [0001] The invention relates to a microfluidic chemiluminescent immunoassay device and a method for using the same, in particular to an immune reaction step of whole blood separation, plasma quantification, mixing (cultivation) and cleaning under a centrifugal system. Background technique [0002] Chemiluminescence immunoassay, also known as luminescence, refers to the light radiation produced by chemical reaction without any excitation of light, heat or electric field. The combination of immune responses is used to detect the content of antigen or antibody in the analyte. Since no external excitation light source is required, background interference can be avoided and the signal-to-noise ratio can be greatly improved. It can be used for the detection of various antigens, antibodies, hormones, enzymes, fatty acids, vitamins, and drugs. It can be used as a substitute for radioimmunoassay and enzyme-linked immunoassay, and is an important development direction...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00
CPCG01N35/00029G01N2035/00158G01N2035/00495
Inventor 杨意枫林佳慧余波
Owner ZHEJIANG PUSHKANG BIOTECHNOLOGY CO LTD
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